LOCUS       sequence-441209-       22027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22027)
  AUTHORS   Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, 
            Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B,
            Zweifel LS, Golden SA
  TITLE     An arginine-rich nuclear localization signal (ArgiNLS) strategy for 
            streamlined image segmentation of single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2320250121. doi: 
            10.1073/pnas.2320250121. Epub 2024 Jul 29.
  PUBMED    39074275
REFERENCE   2  (bases 1 to 22027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-6"; volume: "121(32):e2320250121. 
            doi"; pages: " 10.1073/pnas.2320250121. Epub 2024 Jul 29"
COMMENT     Sequence Label: pDG1-PHP.eB
FEATURES             Location/Qualifiers
     source          1..22027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     483..499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     483..499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5289..5300
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      7699..7708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   19924..20026
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(20217..20805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20297..20316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(20957..21748,21749..21817))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21580..21599
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21818..21922)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     21990..22008
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
       61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
      121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
      181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
      241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
      301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
      361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
      421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
      481 cctctagaac tagtggatcc gtccctagaa gtaaaaaagg gaaaaaagag tgtgtttgtc
      541 aaaataggag acaggtggtg gcaaccaggg acttataggg gaccttacat ctacagacca
      601 acagatgccc ccttaccata tacaggaaga tatgacttaa attgggatag gtgggtcaca
      661 atcaacggct ataaagtgtt atacagatcc ctcccctttc gtgaaagact cgccagagct
      721 agacctcctt ggtgtatgct aactgagaaa gagaaagacg acatgaaaca acaggtacat
      781 gattatattt atctaggaac aggaatgcac ttttggggaa aggttttcca taccaaggaa
      841 ggggcagtgg ctggactgat agaacattat tctgcaaaaa cttatggtat gagttattat
      901 gattagcctt tatttgccca accttgcggt tcccagggtt taaataagtt tatggttaca
      961 aactgttctt aaaacaagga tgtgagacaa gtggtttcct gagttggttt ggtatcaaat
     1021 gttctgatct gagctcttag tgttctattt tcctatgttc ttttggaatc tatccaagtc
     1081 ttatgtaaat gcttatgtaa accataatat aaaagagtgc tgattttttg agtaaacttg
     1141 caacagtcct aacattcttc tctcgtgtgt ttgtgtctgt tcgccatccc gtctccgctc
     1201 gtcacttatc cttcactttt cagagggtcc ccccgcagat cccggtcacc ctcaggtcgg
     1261 gacctgcaga agacgcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
     1321 acgcgcagcc gccatgccgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
     1381 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
     1441 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
     1501 cgagaagctg cagcgcgact ttctgacgga atggcgccgt gtgagtaagg ccccggaggc
     1561 ccttttcttt gtgcaatttg agaagggaga gagctacttc cacatgcacg tgctcgtgga
     1621 aaccaccggg gtgaaatcca tggttttggg acgtttcctg agtcagattc gcgaaaaact
     1681 gattcagaga atttaccgcg ggatcgagcc gactttgcca aactggttcg cggtcacaaa
     1741 gaccagaaat ggcgccggag gcgggaacaa ggtggtggat gagtgctaca tccccaatta
     1801 cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg actaatatgg aacagtattt
     1861 aagcgcctgt ttgaatctca cggagcgtaa acggttggtg gcgcagcatc tgacgcacgt
     1921 gtcgcagacg caggagcaga acaaagagaa tcagaatccc aattctgatg cgccggtgat
     1981 cagatcaaaa acttcagcca ggtacatgga gctggtcggg tggctcgtgg acaaggggat
     2041 tacctcggag aagcagtgga tccaggagga ccaggcctca tacatctcct tcaatgcggc
     2101 ctccaactcg cggtcccaaa tcaaggctgc cttggacaat gcgggaaaga ttatgagcct
     2161 gactaaaacc gcccccgact acctggtggg ccagcagccc gtggaggaca tttccagcaa
     2221 tcggatttat aaaattttgg aactaaacgg gtacgatccc caatatgcgg cttccgtctt
     2281 tctgggatgg gccacgaaaa agttcggcaa gaggaacacc atctggctgt ttgggcctgc
     2341 aactaccggg aagaccaaca tcgcggaggc catagcccac actgtgccct tctacgggtg
     2401 cgtaaactgg accaatgaga actttccctt caacgactgt gtcgacaaga tggtgatctg
     2461 gtgggaggag gggaagatga ccgccaaggt cgtggagtcg gccaaagcca ttctcggagg
     2521 aagcaaggtg cgcgtggacc agaaatgcaa gtcctcggcc cagatagacc cgactcccgt
     2581 gatcgtcacc tccaacacca acatgtgcgc cgtgattgac gggaactcaa cgaccttcga
     2641 acaccagcag ccgttgcaag accggatgtt caaatttgaa ctcacccgcc gtctggatca
     2701 tgactttggg aaggtcacca agcaggaagt caaagacttt ttccggtggg caaaggatca
     2761 cgtggttgag gtggagcatg aattctacgt caaaaagggt ggagccaaga aaagacccgc
     2821 ccccagtgac gcagatataa gtgagcccaa acgggtgcgc gagtcagttg cgcagccatc
     2881 gacgtcagac gcggaagctt cgatcaacta cgcagacagg taccaaaaca aatgttctcg
     2941 tcacgtgggc atgaatctga tgctgtttcc ctgcagacaa tgcgagagaa tgaatcagaa
     3001 ttcaaatatc tgcttcactc acggacagaa agactgttta gagtgctttc ccgtgtcaga
     3061 atctcaaccc gtttctgtcg tcaaaaaggc gtatcagaaa ctgtgctaca ttcatcatat
     3121 catgggaaag gtgccagacg cttgcactgc ctgcgatctg gtcaatgtgg atttggatga
     3181 ctgcatcttt gaacaataaa tgatttaaat caggtatggc tgccgatggt tatcttccag
     3241 attggctcga ggacaacctc tctgagggca ttcgcgagtg gtgggctttg aaacctggag
     3301 cccctcaacc caaggcaaat caacaacatc aagacaacgc tagaggtctt gtgcttccgg
     3361 gttacaaata ccttggaccc ggcaacggac tcgacaaggg ggagccggtc aacgcagcag
     3421 acgcggcggc cctcgagcac gacaaagcct acgaccagca gctcaaggcc ggagacaacc
     3481 cgtacctcaa gtacaaccac gccgacgccg agttccagga gcggctcaaa gaagatacgt
     3541 cttttggggg caacctcggg cgagcagtct tccaggccaa aaagaggctt cttgaacctc
     3601 ttggtctggt tgaggaagcg gctaagacgg ctcctggaaa gaagaggcct gtagagcagt
     3661 ctcctcagga accggactcc tccgcgggta ttggcaaatc gggtgcacag cccgctaaaa
     3721 agagactcaa tttcggtcag actggcgaca cagagtcagt cccagaccct caaccaatcg
     3781 gagaacctcc cgcagccccc tcaggtgtgg gatctcttac aatggcttca ggtggtggcg
     3841 caccagtggc agacaataac gaaggtgccg atggagtggg tagttcctcg ggaaattggc
     3901 attgcgattc ccaatggctg ggggacagag tcatcaccac cagcacccga acctgggccc
     3961 tgcccaccta caacaatcac ctctacaagc aaatctccaa cagcacatct ggaggatctt
     4021 caaatgacaa cgcctacttc ggctacagca ccccctgggg gtattttgac ttcaacagat
     4081 tccactgcca cttctcacca cgtgactggc agcgactcat caacaacaac tggggattcc
     4141 ggcctaagcg actcaacttc aagctcttta acattcaggt caaagaggtt acggacaaca
     4201 atggagtcaa gaccatcgcc aataacctta ccagcacggt ccaggtcttc acggactcag
     4261 actatcagct cccgtacgtg ctcgggtcgg ctcacgaggg ctgcctcccg ccgttcccag
     4321 cggacgtttt catgattcct cagtacgggt atctgacgct taatgatgga agccaggccg
     4381 tgggtcgttc gtccttttac tgcctggaat atttcccgtc gcaaatgcta agaacgggta
     4441 acaacttcca gttcagctac gagtttgaga acgtaccttt ccatagcagc tacgctcaca
     4501 gccaaagcct ggaccgacta atgaatccac tcatcgacca atacttgtac tatctctcta
     4561 gaactattaa cggttctgga cagaatcaac aaacgctaaa attcagtgtg gccggaccca
     4621 gcaacatggc tgtccaggga agaaactaca tacctggacc cagctaccga caacaacgtg
     4681 tctcaaccac tgtgactcaa aacaacaaca gcgaatttgc ttggcctgga gcttcttctt
     4741 gggctctcaa tggacgtaat agcttgatga atcctggacc tgctatggcc tctcacaaag
     4801 aaggagagga ccgtttcttt cctttgtctg gatctttaat ttttggcaaa caaggtactg
     4861 gcagagacaa cgtggatgcg gacaaagtca tgataaccaa cgaagaagaa attaaaacta
     4921 ctaacccggt agcaacggag tcctatggac aagtggccac aaaccaccag agtgatggga
     4981 ctttggcggt gccttttaag gcacaggcgc agaccggttg ggttcaaaac caaggaatac
     5041 ttccgggtat ggtttggcag gacagagatg tgtacctgca aggacccatt tgggccaaaa
     5101 ttcctcacac ggacggcaac tttcaccctt ctccgctgat gggagggttt ggaatgaagc
     5161 acccgcctcc tcagatcctc atcaaaaaca cacctgtacc tgcggatcct ccaacggcct
     5221 tcaacaagga caagctgaac tctttcatca cccagtattc tactggtcaa gtcagcgtgg
     5281 agatcgagtg ggagctgcag aaggaaaaca gcaagcgctg gaacccggag atccagtaca
     5341 cttccaacta ttacaagtct aataatgttg aatttgctgt taatactgaa ggtgtatata
     5401 gtgaaccccg ccccattggc accagatacc tgactcgtaa tctgtaagtc gacttgcttg
     5461 ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg gtctctgcga agggcaattc
     5521 gtttaaacct gcaggactag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
     5581 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
     5641 tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat atcacatgtc
     5701 ctaggaacta tcgatgcatg tccttgggtc cggcctgctg aatgcgcagg cggtcggcca
     5761 tgccccaggc ttcgttttga catcggcgca ggtctttgta gtagtcttgc atgagccttt
     5821 ctaccggcac ttcttcttct ccttcctctt gtcctgcatc tcttgcatct atcgctgcgg
     5881 cggcggcgga gtttggccgt aggtggcgcc ctcttcctcc catcgtgtga ccccgaagcc
     5941 cctcatcggc tgaagcaggg ctaggtcggc gacaacgcgc tcggctaata tggcctgctg
     6001 cacctgcgtg agggtagact ggaagtcatc catgtccaca aagcggtggt atgcgcccgt
     6061 gttgatggtg taagtgcagt tggccataac ggaccagtta acggtctggt gacccggctg
     6121 cgagagctcg gtgtacctga gacgcgagta agccctcgag tcaaatacgt agtcgttaca
     6181 agtccgcacc aggtactggt atcccaccaa aaagtgcggc ggcggctggc ggtagagggg
     6241 ccagcgtagg gtggccgggg ctccgggggc gagatcttcc aacataaggc gatgatatcc
     6301 gtagatgtac ctggacatcc aggtgatgcc ggcggcggtg gtggaggcgc gcggaaagtc
     6361 gcggacgcgg ttccagatgt tgcgcagcgg caaaaagtgc tccatggtcg ggacgctctg
     6421 gccggtcagg cgcgcgcaat cgttgacgct ctagaccgtg caaaaggaga gcctgtaagc
     6481 gggcactctt ccgtggtctg gtggataaat tcgcaagggt atcatggcgg acgaccgggg
     6541 ttcgagcccc gtatccggcc gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac
     6601 ccaggtgtgc gacgtcagac aacgggggag tgctcctttt ggcttccttc caggcgcggc
     6661 ggctgctgcg ctagcttttt tggccactgg ccgcgcgcag cgtaagcggt taggctggaa
     6721 agcgaaagca ttaagtggct cgctccctgt agccggaggg ttattttcca agggttgagt
     6781 cgcgggaccc ccggttcgag tctcggaccg gccggactgc ggcgaacggg ggtttgtctc
     6841 cccgtcatgc aagaccccgc ttgcaaattc ctccggaaac agggacgagc cccttttttg
     6901 cttttcccag atgcatccgg tgctgcggca gatgcgcccc cctcctcagc agcggcaaga
     6961 gcaagagcag cggcagacat gcagggcacc ctcccctcct cctaccgcgt caggaggggc
     7021 gacatccgcg gttgacgcgg cagcagatgg tgattacgaa cccccgcggc gccgggcccg
     7081 gcactacctg gacttggagg agggcgaggg cctggcgcgg ctaggagcgc cctctcctga
     7141 gcggcaccca agggtgcagc tgaagcgtga tacgcgtgag gcgtacgtgc cgcggcagaa
     7201 cctgtttcgc gaccgcgagg gagaggagcc cgaggagatg cgggatcgaa agttccacgc
     7261 agggcgcgag ctgcggcatg gcctgaatcg cgagcggttg ctgcgcgagg aggactttga
     7321 gcccgacgcg cgaaccggga ttagtcccgc gcgcgcacac gtggcggccg ccgacctggt
     7381 aaccgcatac gagcagacgg tgaaccaggg cgatcgcacc ctttggcgca tcccattctc
     7441 cagtaacttt atgtccatgg gcgcactcac agacctgggc caaaaccttc tctacgccaa
     7501 ctccgcccac gcgctagaca tgacttttga ggtggatccc atggacgagc ccacccttct
     7561 ttatgttttg tttgaagtct ttgacgtggt ccgtgtgcac cagccgcacc gcggcgtcat
     7621 cgaaaccgtg tacctgcgca cgcccttctc ggccggcaac gccacaacat aaagaagcaa
     7681 gcaacatcaa caacagctgc cgccatgggc tccagtgagc aggaactgaa agccattgtc
     7741 aaagatcttg gttgtgggcc atattttttg ggcacctatg acaagcgctt tccaggcttt
     7801 gtttctccac acaagctcgc ctgcgccata gtcaatacgg ccggtcgcga gactgggggc
     7861 gtacactgga tggcctttgc ctggaacccg cactcaaaaa catgctacct ctttgagccc
     7921 tttggctttt ctgaccagcg actcaagcag gtttaccagt ttgagtacga gtcactcctg
     7981 cgccgtagcg ccattgcttc ttcccccgac cgctgtataa cgctggaaaa gtccacccaa
     8041 agcgtacagg ggcccaactc ggccgcctgt ggactattct gctgcatgtt tctccacgcc
     8101 tttgccaact ggccccaaac tcccatggat cacaacccca ccatgaacct tattaccggg
     8161 gtacccaact ccatgctcaa cagtccccag gtacagccca ccctgcgtcg caaccaggaa
     8221 cagctctaca gcttcctgga gcgccactcg ccctacttcc gcagccacag tgcgcagatt
     8281 aggagcgcca cttctttttg tcacttgaaa aacatgtaaa aataatgtac tagagacact
     8341 ttcaataaag gcaaatgctt ttatttgtac actctcgggt gattatttac ccccaccctt
     8401 gccgtctgcg ccgtttaaaa atcaaagggg ttctgccgcg catcgctatg cgccactggc
     8461 agggacacgt tgcgatactg gtgtttagtg ctccacttaa actcaggcac aaccatccgc
     8521 ggcagctcgg tgaagttttc actccacagg ctgcgcacca tcaccaacgc gtttagcagg
     8581 tcgggcgccg atatcttgaa gtcgcagttg gggcctccgc cctgcgcgcg cgagttgcga
     8641 tacacagggt tgcagcactg gaacactatc agcgccgggt ggtgcacgct ggccagcacg
     8701 ctcttgtcgg agatcagatc cgcgtccagg tcctccgcgt tgctcagggc gaacggagtc
     8761 aactttggta gctgccttcc caaaaagggc gcgtgcccag gctttgagtt gcactcgcac
     8821 cgtagtggca tcaaaaggtg accgtgcccg gtctgggcgt taggatacag cgcctgcata
     8881 aaagccttga tctgcttaaa agccacctga gcctttgcgc cttcagagaa gaacatgccg
     8941 caagacttgc cggaaaactg attggccgga caggccgcgt cgtgcacgca gcaccttgcg
     9001 tcggtgttgg agatctgcac cacatttcgg ccccaccggt tcttcacgat cttggccttg
     9061 ctagactgct ccttcagcgc gcgctgcccg ttttcgctcg tcacatccat ttcaatcacg
     9121 tgctccttat ttatcataat gcttccgtgt agacacttaa gctcgccttc gatctcagcg
     9181 cagcggtgca gccacaacgc gcagcccgtg ggctcgtgat gcttgtaggt cacctctgca
     9241 aacgactgca ggtacgcctg caggaatcgc cccatcatcg tcacaaaggt cttgttgctg
     9301 gtgaaggtca gctgcaaccc gcggtgctcc tcgttcagcc aggtcttgca tacggccgcc
     9361 agagcttcca cttggtcagg cagtagtttg aagttcgcct ttagatcgtt atccacgtgg
     9421 tacttgtcca tcagcgcgcg cgcagcctcc atgcccttct cccacgcaga cacgatcggc
     9481 acactcagcg ggttcatcac cgtaatttca ctttccgctt cgctgggctc ttcctcttcc
     9541 tcttgcgtcc gcataccacg cgccactggg tcgtcttcat tcagccgccg cactgtgcgc
     9601 ttacctcctt tgccatgctt gattagcacc ggtgggttgc tgaaacccac catttgtagc
     9661 gccacatctt ctctttcttc ctcgctgtcc acgattacct ctggtgatgg cgggcgctcg
     9721 ggcttgggag aagggcgctt ctttttcttc ttgggcgcaa tggccaaatc cgccgccgag
     9781 gtcgatggcc gcgggctggg tgtgcgcggc accagcgcgt cttgtgatga gtcttcctcg
     9841 tcctcggact cgatacgccg cctcatccgc ttttttgggg gcgcccgggg aggcggcggc
     9901 gacggggacg gggacgacac gtcctccatg gttgggggac gtcgcgccgc accgcgtccg
     9961 cgctcggggg tggtttcgcg ctgctcctct tcccgactgg ccatttcctt ctcctatagg
    10021 cagaaaaaga tcatggagtc agtcgagaag aaggacagcc taaccgcccc ctctgagttc
    10081 gccaccaccg cctccaccga tgccgccaac gcgcctacca ccttccccgt cgaggcaccc
    10141 ccgcttgagg aggaggaagt gattatcgag caggacccag gttttgtaag cgaagacgac
    10201 gaggaccgct cagtaccaac agaggataaa aagcaagacc aggacaacgc agaggcaaac
    10261 gaggaacaag tcgggcgggg ggacgaaagg catggcgact acctagatgt gggagacgac
    10321 gtgctgttga agcatctgca gcgccagtgc gccattatct gcgacgcgtt gcaagagcgc
    10381 agcgatgtgc ccctcgccat agcggatgtc agccttgcct acgaacgcca cctattctca
    10441 ccgcgcgtac cccccaaacg ccaagaaaac ggcacatgcg agcccaaccc gcgcctcaac
    10501 ttctaccccg tatttgccgt gccagaggtg cttgccacct atcacatctt tttccaaaac
    10561 tgcaagatac ccctatcctg ccgtgccaac cgcagccgag cggacaagca gctggccttg
    10621 cggcagggcg ctgtcatacc tgatatcgcc tcgctcaacg aagtgccaaa aatctttgag
    10681 ggtcttggac gcgacgagaa gcgcgcggca aacgctctgc aacaggaaaa cagcgaaaat
    10741 gaaagtcact ctggagtgtt ggtggaactc gagggtgaca acgcgcgcct agccgtacta
    10801 aaacgcagca tcgaggtcac ccactttgcc tacccggcac ttaacctacc ccccaaggtc
    10861 atgagcacag tcatgagtga gctgatcgtg cgccgtgcgc agcccctgga gagggatgca
    10921 aatttgcaag aacaaacaga ggagggccta cccgcagttg gcgacgagca gctagcgcgc
    10981 tggcttcaaa cgcgcgagcc tgccgacttg gaggagcgac gcaaactaat gatggccgca
    11041 gtgctcgtta ccgtggagct tgagtgcatg cagcggttct ttgctgaccc ggagatgcag
    11101 cgcaagctag aggaaacatt gcactacacc tttcgacagg gctacgtacg ccaggcctgc
    11161 aagatctcca acgtggagct ctgcaacctg gtctcctacc ttggaatttt gcacgaaaac
    11221 cgccttgggc aaaacgtgct tcattccacg ctcaagggcg aggcgcgccg cgactacgtc
    11281 cgcgactgcg tttacttatt tctatgctac acctggcaga cggccatggg cgtttggcag
    11341 cagtgcttgg aggagtgcaa cctcaaggag ctgcagaaac tgctaaagca aaacttgaag
    11401 gacctatgga cggccttcaa cgagcgctcc gtggccgcgc acctggcgga catcattttc
    11461 cccgaacgcc tgcttaaaac cctgcaacag ggtctgccag acttcaccag tcaaagcatg
    11521 ttgcagaact ttaggaactt tatcctagag cgctcaggaa tcttgcccgc cacctgctgt
    11581 gcacttccta gcgactttgt gcccattaag taccgcgaat gccctccgcc gctttggggc
    11641 cactgctacc ttctgcagct agccaactac cttgcctacc actctgacat aatggaagac
    11701 gtgagcggtg acggtctact ggagtgtcac tgtcgctgca acctatgcac cccgcaccgc
    11761 tccctggttt gcaattcgca gctgcttaac gaaagtcaaa ttatcggtac ctttgagctg
    11821 cagggtccct cgcctgacga aaagtccgcg gctccggggt tgaaactcac tccggggctg
    11881 tggacgtcgg cttaccttcg caaatttgta cctgaggact accacgccca cgagattagg
    11941 ttctacgaag accaatcccg cccgcctaat gcggagctta ccgcctgcgt cattacccag
    12001 ggccacattc ttggccaatt gcaagccatc aacaaagccc gccaagagtt tctgctacga
    12061 aagggacggg gggtttactt ggacccccag tccggcgagg agctcaaccc aatccccccg
    12121 ccgccgcagc cctatcagca gcagccgcgg gcccttgctt cccaggatgg cacccaaaaa
    12181 gaagctgcag ctgccgccgc cacccacgga cgaggaggaa tactgggaca gtcaggcaga
    12241 ggaggttttg gacgaggagg aggaggacat gatggaagac tgggagagcc tagacgagga
    12301 agcttccgag gtcgaagagg tgtcagacga aacaccgtca ccctcggtcg cattcccctc
    12361 gccggcgccc cagaaatcgg caaccggttc cagcatggct acaacctccg ctcctcaggc
    12421 gccgccggca ctgcccgttc gccgacccaa ccgtagatgg gacaccactg gaaccagggc
    12481 cggtaagtcc aagcagccgc cgccgttagc ccaagagcaa caacagcgcc aaggctaccg
    12541 ctcatggcgc gggcacaaga acgccatagt tgcttgcttg caagactgtg ggggcaacat
    12601 ctccttcgcc cgccgctttc ttctctacca tcacggcgtg gccttccccc gtaacatcct
    12661 gcattactac cgtcatctct acagcccata ctgcaccggc ggcagcggca gcaacagcag
    12721 cggccacaca gaagcaaagg cgaccggata gcaagactct gacaaagccc aagaaatcca
    12781 cagcggcggc agcagcagga ggaggagcgc tgcgtctggc gcccaacgaa cccgtatcga
    12841 cccgcgagct tagaaacagg atttttccca ctctgtatgc tatatttcaa cagagcaggg
    12901 gccaagaaca agagctgaaa ataaaaaaca ggtctctgcg atccctcacc cgcagctgcc
    12961 tgtatcacaa aagcgaagat cagcttcggc gcacgctgga agacgcggag gctctcttca
    13021 gtaaatactg cgcgctgact cttaaggact agtttcgcgc cctttctcaa atttaagcgc
    13081 gaaaactacg tcatctccag cggccacacc cggcgccagc acctgttgtc agcgccatta
    13141 tgagcaagga aattcccacg ccctacatgt ggagttacca gccacaaatg ggacttgcgg
    13201 ctggagctgc ccaagactac tcaacccgaa taaactacat gagcgcggga ccccacatga
    13261 tatcccgggt caacggaata cgcgcccacc gaaaccgaat tctcctggaa caggcggcta
    13321 ttaccaccac acctcgtaat aaccttaatc cccgtagttg gcccgctgcc ctggtgtacc
    13381 aggaaagtcc tgctcccacc actgtggtac ttcccagaga cgcccaggcc gaagttcaga
    13441 tgactaactc aggggcgcag cttgcgggcg gctttcgtca cagggtgcgg tcgcccgggc
    13501 agggtataac tcacctgaca atcagagggc gaggtattca gctcaacgac gagtcggtga
    13561 gctcctcgct tggtctccgt ccggacggga catttcagat cggcggcgcc ggccgctctt
    13621 cattcacgcc tcgtcaggca atcctaactc tgcagacctc gtcctctgag ccgcgctctg
    13681 gaggcattgg aactctgcaa tttattgagg agtttgtgcc atcggtctac tttaacccct
    13741 tctcgggacc tcccggccac tatccggatc aatttattcc taactttgac gcggtaaagg
    13801 actcggcgga cggctacgac tgaatgttaa gtggagaggc agagcaactg cgcctgaaac
    13861 acctggtcca ctgtcgccgc cacaagtgct ttgcccgcga ctccggtgag ttttgctact
    13921 ttgaattgcc cgaggatcat atcgagggcc cggcgcacgg cgtccggctt accgcccagg
    13981 gagagcttgc ccgtagcctg attcgggagt ttacccagcg ccccctgcta gttgagcggg
    14041 acaggggacc ctgtgttctc actgtgattt gcaactgtcc taaccctgga ttacatcaag
    14101 atctttgttg ccatctctgt gctgagtata ataaatacag aaattaaaat atactggggc
    14161 tcctatcgcc atcctgtaaa cgccaccgtc ttcacccgcc caagcaaacc aaggcgaacc
    14221 ttacctggta cttttaacat ctctccctct gtgatttaca acagtttcaa cccagacgga
    14281 gtgagtctac gagagaacct ctccgagctc agctactcca tcagaaaaaa caccaccctc
    14341 cttacctgcc gggaacgtac gagtgcgtca ccggccgctg caccacacct accgcctgac
    14401 cgtaaaccag actttttccg gacagacctc aataactctg tttaccagaa caggaggtga
    14461 gcttagaaaa cccttagggt attaggccaa aggcgcagct actgtggggt ttatgaacaa
    14521 ttcaagcaac tctacgggct attctaattc aggtttctct agaaatggac ggaattatta
    14581 cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc atgaatcaag
    14641 agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt ctggtaaagc
    14701 aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac aagttgccaa
    14761 ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata actcagcact
    14821 cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat ctctgcaccc
    14881 ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa aaaaaaataa
    14941 taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt cagcagcacc
    15001 tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa ctttctccac
    15061 aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac tatcttcatg
    15121 ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt gtatccatat
    15181 gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt atcccccaat
    15241 gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc tctagttacc
    15301 tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga ggccggcaac
    15361 cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa gtcaaacata
    15421 aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt ggctgccgcc
    15481 gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc gctaaccgtg
    15541 cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga aggaaagcta
    15601 gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac tatcactgcc
    15661 tcaccccctc taactactgc cactggtagc ttgggcattg acttgaaaga gcccatttat
    15721 acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac agacgaccta
    15781 aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc cttgcaaact
    15841 aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa tgtagcagga
    15901 ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta tccgtttgat
    15961 gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa ctcagcccac
    16021 aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa caattccaaa
    16081 aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac agccatagcc
    16141 attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac aaatcccctc
    16201 aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt tcctaaacta
    16261 ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa aaataatgat
    16321 aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa tgcagagaaa
    16381 gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc tacagtttca
    16441 gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag tgctcatctt
    16501 attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga cccagaatat
    16561 tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc tgttggattt
    16621 atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag taacattgtc
    16681 agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat tacactaaac
    16741 ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt ttcatgggac
    16801 tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac tttttcatac
    16861 attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt ttcaattgca
    16921 gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata gcttatacag
    16981 atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac ctccctccca
    17041 acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt
    17101 aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc
    17161 agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg
    17221 agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc
    17281 ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc
    17341 gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc
    17401 ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg cacagcagcg
    17461 caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa
    17521 aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg
    17581 gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat
    17641 aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg
    17701 attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc
    17761 tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc
    17821 atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca
    17881 cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa caacccattc
    17941 ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat
    18001 tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtttc
    18061 tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca accgagatcg
    18121 tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctgaagcaaa
    18181 accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta gatcgctctg
    18241 tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg gcttcgggtt
    18301 ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca gaataagcca
    18361 cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg ggaagagctg
    18421 gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa atgaagatct
    18481 attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa agaacagata
    18541 atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct cacgtccaag
    18601 tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc agcaccttca
    18661 accatgccca aataattctc atctcgccac cttctcaata tatctctaag caaatcccga
    18721 atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt cagcctcaag
    18781 cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga ttcaaaagcg
    18841 gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc tgaacataat
    18901 cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg acaaaagaac
    18961 ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc ccgatgtaag
    19021 cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca ggcaaagcct
    19081 cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag gtaagctccg
    19141 gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt ttctgcataa
    19201 acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta caacaggaaa
    19261 aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt aaaaaaactg
    19321 gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag tcataatgta
    19381 agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc gaccgaaata
    19441 gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca taggaggtat
    19501 aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct gcctaggcaa
    19561 aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag ccataacagt
    19621 cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct
    19681 caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa
    19741 tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca
    19801 gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta
    19861 cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa
    19921 aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca
    19981 ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa ttaagaattc
    20041 ggatctgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
    20101 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
    20161 cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    20221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    20281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    20341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    20401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    20461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    20521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    20581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    20641 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    20701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    20761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    20821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    20881 agattatcaa aaaggatctt cacctagatc cttttaaatc aatctaaagt atatatgagt
    20941 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    21001 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    21061 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    21121 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    21181 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    21241 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    21301 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    21361 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    21421 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    21481 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    21541 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    21601 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    21661 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    21721 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    21781 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    21841 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    21901 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    21961 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    22021 aagaatt
//
