LOCUS       sequence-441211-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral guide RNA vector to insert the loxPsym sequence into 
            LINE1 elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Koeppel J, Ferreira R, Vanderstichele T, Riedmayr LM, Peets EM, 
            Girling G, Weller J, Murat P, Liberante FG, Ellis T, Church GM, 
            Parts L
  TITLE     Randomizing the human genome by engineering recombination between 
            repeat elements.
  JOURNAL   Science. 2025 Jan 31;387(6733):eado3979. doi: 
            10.1126/science.ado3979. Epub 2025 Jan 31.
  PUBMED    39883775
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-01-31"; volume: "387(6733):eado3979. doi"; pages: " 
            10.1126/science.ado3979. Epub 2025 Jan 31"
COMMENT     Sequence Label: pLenti-ePEG-LINE1-loxPsym
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      144..732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     633..652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     886..903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1020..1041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1056..1061,1062..1079,1080..1086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1094..1110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1099..1121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1118..1134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1118..1134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1153..1173
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1155..1173
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1201..1427
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1428..1608
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1655..1780
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2273..2506
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2691..2735
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2913..3161
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2913..2933
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3084..3103
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    3302..3335
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     misc_feature    3534..3651
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3790..4968
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4021..4959
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4916..4936
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4981..5580
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4981..5000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5477..5497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5596..6184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5649..5669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6067..6078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6256..6489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6567..6688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6604..6623)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6658..6677
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6728..6863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6883..6902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6884..6902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6912..6929)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6912..6928)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6921..6943)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7070..7525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7157..7176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7367..7388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7551..7655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7656..7724,7725..8516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7874..7893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
       61 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      121 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      181 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      241 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      301 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      361 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      421 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      481 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      541 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      601 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      661 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      721 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      781 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      841 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      901 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      961 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1021 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1081 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1141 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1201 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     1261 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     1321 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     1381 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     1441 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1501 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1561 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1621 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1681 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1741 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1801 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1861 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1921 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1981 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2041 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2101 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2161 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2221 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2281 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2341 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2401 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2461 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2521 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2581 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2641 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2701 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2761 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2821 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2881 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     2941 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     3001 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     3061 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     3121 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgttctacca gaggtacaag
     3181 ggtttaagag ctaagctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     3241 ctcgaaagag tggcaccgag tcggtgcaat agtttcagaa ggaatggtac cagttcctcc
     3301 tataacttcg tataatgtac attatacgaa gttattgtac ctctggtacg cggttctatc
     3361 tagttacgcg ttaaaccaac tagaattttt ttaagcctac tctaccactt gtacttcagc
     3421 ggtcagctta ctcgacttaa cgtgcacgtg acacgttcta gaccgtacat gcttacatgg
     3481 gatgaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
     3541 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     3601 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     3661 tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg
     3721 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     3781 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3841 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     3901 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3961 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4021 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     4081 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     4141 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     4201 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     4261 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     4321 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     4381 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     4441 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     4501 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     4561 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     4621 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     4681 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     4741 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     4801 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     4861 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     4921 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg tacggccacc
     4981 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5041 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5101 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5161 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     5221 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5281 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5341 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     5401 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5461 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5521 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5581 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5641 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5701 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5761 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5821 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5881 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5941 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6001 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6061 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6121 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6181 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6481 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6541 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6601 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6661 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6781 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6841 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6901 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6961 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7021 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7501 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7561 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8521 cagaccaagt ttactcatat ata
//
