LOCUS       sequence-441228-        4976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT1BR coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             348..395
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             429..476
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             492..539
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             555..602
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             618..665
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             681..728
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             744..791
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             795..821
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2012..2028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2012..2028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2025..2047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2200..2788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2280..2299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2905..2929)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3029..3820,3821..3889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3652..3671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3890..3994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4093..4115
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4107..4124
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4108..4124
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctacttcaa ctcgaccttg aatccggtat gtaaattagc ccctggctcg ttccctctcc
       61 tcgcagctta agctcacttt aaacctttac cctttcgagc aaattgcttt ggcatgaaat
      121 agcttcagtt tagaacattt tttttattga tatgaatacc attttttatt taaattttct
      181 ataaatattt gcctttatgc caggtcatct acaccatctt caatccagaa tttcgacggg
      241 ccttcaagag aattctcttc ggtcgaaagg ctgccgctcg agcgcgcagt gcgaaaattt
      301 ccggcgatgg aggaagcgga ggcgacggag gcagcggagg aggcagccgg gaccacatgg
      361 tgctgcacga gtacgtgaac gccgccggca tcactagcgg aggcgacgga ggcagcggag
      421 gaggcagccg ggaccacatg gtgctgcacg agtacgtgaa cgccgccggc atcactagcg
      481 gtagtggcgg ccgtgaccac atggtccttc atgagtatgt aaatgctgct gggattacag
      541 gtggctctgg aggtagagat catatggttc tccacgaata cgttaacgcc gcaggcatca
      601 ctggcggtag tggaggacgc gaccatatgg tactacatga atatgtcaat gcagccggaa
      661 taaccggagg gtccggaggc cgggatcaca tggtgctgca tgagtatgtg aacgcggcgg
      721 gtataactgg tgggtcgggc ggacgagacc atatggtgct tcacgaatac gtaaacgcag
      781 ctggcattac tagttaccca tacgatgttc cagattacgc ttaatgaaga ataacgagga
      841 ccagtatttg taattaaggt caggcgaagg agtaaaggtg gtctatacag acgggcaggc
      901 aatatgctac ggaattggca ttgtataaag gcaaactagc aagtagagaa gatgttttag
      961 cagtgacaat cctaactagc tgtaactaat taactaaaat tcctcaccac acacacgtgc
     1021 ccctaaaaga caaaaaaagc gaaatatgaa tagaaacaaa acttaaatgt aaccgaaacc
     1081 aaacgagaaa tgaaaacgtg gaattatggt agctgatact cactagatct aagctatgcg
     1141 tatgtattag taatttatag atatttctaa agtcttccct gtgtcttaaa gaaacccaag
     1201 agtagtacgc ttggataccc tagaaaccct aagtaggtgg tatgaacagt gatcggagat
     1261 cgaagatagt gatcggttat atataggctg ggtccaaaga acttcttacg ctttcatatt
     1321 ctgtgttagt taatcgcgtt tagttatagc ccaagctgca aaatgctcaa ttatttcaat
     1381 tggataagca catttacata aaggtcaata tcaacaaaga ttaaacgaat ttacacagtt
     1441 aactcatgac gacactaaaa accgaaacca aaattttgca taaccccata aaggataagc
     1501 caccaacgca atggctaatt aggcagtaca cacaacttta ttcggctaaa ttagcataaa
     1561 tccaaaacgg aaagttgaac tttcgttact aatatttctc atacacatgc atatgcgtgc
     1621 catttgaaat ccaggaagcc gcaacaaaaa gtttgaccca tgatacacgc attcatagtg
     1681 gtacagcatt acatatatac atatatacat atatatatat ttagtatagt acatttattg
     1741 ttcgattttt gaaaagtttt cttgtaaata aacggactta cagttttagc aaatagtaaa
     1801 ccaaccgaaa aacctacaac cacaacaaga gcaacaacca ctcgaataac aaccatggat
     1861 atatacacgt cggtctgtat gtaagggcca ttaagtcaat gctgcagttg aaacctaata
     1921 cgcttcggta tgcacatgta tgcggccgca attataggct aggtggaggc tcagtgatga
     1981 taagtctgcg atggtggatg catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt
     2041 atccgctcag agggcacaat cctattccgc gctatccgac aatctccaag acattaggtg
     2101 gagttcagtt cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa aaggccagca
     2161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2641 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2821 cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa
     2881 atcgtcctcg tgataccaat tcggagcctg cttttttgta caaacttgtt gataatggca
     2941 attcaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gagccacgct
     3181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3481 gaagtaagtt ggccgcagtg ttatcactca tggttatggt agcactgcat aattctctta
     3541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccagata
     4021 cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat ccaccacaag
     4081 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca taatccgcac
     4141 gcatctggaa ggaagtgcca ttccgcctga cctaattatg ctagcgatgc ggatgatgta
     4201 agtacgccca ttgggctgga atataattaa gagctgcaag tagggtgcca ctttaagcca
     4261 cggaatattt tcttaaggaa aacgacattt aagttaactg aaattaaagc tgagtaaata
     4321 tgaaattttt tcaagcagac agattaaata agtcaaaacc atttaagtgc ttaagaaggt
     4381 tctgctatgg cagtttgttc gtcttatatt catagtcatt tgctcgtggc gcgccctttg
     4441 ttcgtgtgag attaacccat gatacccccg cttaacccct tagtatcgca ccagcaacgc
     4501 gaatgaaatc atcacgctgt cgcagcaggt ggcccatgcc acgcagcacc atctgatagc
     4561 ctcgcatctg aatgccatta cgccgctggc ccagtccatc gccatgggtg gtgttggttg
     4621 cctgaccacg accacgccat cggagaaggc actgtcagga gcaggaacgg tagcgggagc
     4681 ggtagctggc ggaagtggaa gcggaagtgg tgaggaggga gccggaacgg agggcaagaa
     4741 tgccggagtg gggctgggcg gagtgctggc cagcattgcc aatccgcacc agaaactggc
     4801 caagcggcga cagctcctgg aggcgaagag ggagagaaag gccgcccaga cgctggccat
     4861 catcaccggc gccttcgtca tctgctggct gccattcttc gtaatggccc tcaccatgag
     4921 cctgtgcaag gagtgcgaga tccacacggc ggtggcatcg ctcttcctct ggctgg
//
