LOCUS       sequence-441233-        9695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Uijttewaal ECH, Lee J, Sell AC, Botay N, Vainorius G, Novatchkova M,
            Baar J, Yang J, Potzler T, van der Leij S, Lowden C, Sinner J, 
            Elewaut A, Gavrilovic M, Obenauf A, Schramek D, Elling U
  TITLE     CRISPR-StAR enables high-resolution genetic screening in complex in 
            vivo models.
  JOURNAL   Nat Biotechnol. 2024 Dec 16. doi: 10.1038/s41587-024-02512-9.
  PUBMED    39681701
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Dec 16. doi: 10.1038/s41587-024-02512-9."
COMMENT     Sequence Label: CRISPR-StAR4B (Lenti)
FEATURES             Location/Qualifiers
     source          1..9695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(190..981,982..1050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     813..832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1051..1155)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1230..1249)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1425..1804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1806..2004
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1959..1979
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2022..2202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2249..2374
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2867..3100
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3285..3329
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3627..3744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3872..4120
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3872..3892
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4043..4062
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(4164..4197)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4325..4358
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(4427..4450)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4777..4796
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4858..5256
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(5265..5298)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5378..5411
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    5570..6158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5623..5643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6041..6052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6161..6177)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6162..6178)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6683..6863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6889..6906)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6895..7119
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7165..7593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7252..7271)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7462..7483
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7602..7622)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7607..7936
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7787..7922
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7849..7868
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7984..8031
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8050..8424
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8554..8675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8591..8610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8645..8664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8724..8740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8724..8740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8737..8759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8748..8764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8772..8778,8779..8796,8797..8802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8817..8838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8955..8972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9126..9695,1..19))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9206..9225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1201 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgcg cgttgacatt gattattgac
     1441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     1501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     1861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1981 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     2041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2221 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2281 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2341 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2401 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2461 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2521 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2581 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2641 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2701 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2761 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2821 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2881 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2941 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3001 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3061 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3121 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3181 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3241 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3301 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3361 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3421 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3481 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3541 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3601 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3661 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3721 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acaggctagc ctctaggcgc
     3781 cggaattgaa gatctccgcg gttaattaaa atgatacggc gaccaccgag atctgtcttg
     3841 atctaggagc tactcatcaa cggtcacatg tgagggccta tttcccatga ttccttcata
     3901 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3961 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     4021 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     4081 tttcttggct ttatatatct tgtggaaagg acgaaacacc cgagacgtct accgcacgtc
     4141 gtctcagttt aagagctatg ctgataactt cgtatagcat acattatacg aagttatttt
     4201 ttttttttgc ggccgccaag agagcagaaa tagcaagtaa ctcattaggt aaataaggta
     4261 gtccgtttag tgccgtagcc aattctcaga catttaatgg ctctaaacgt aacatttaca
     4321 aattataact tcgtataatg tgtactatac gaagttatta attaattctg gttcgaatgt
     4381 agaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4441 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4501 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4561 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4621 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4681 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttcc gggctcaggg
     4741 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     4801 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaatatg
     4861 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     4921 agcatcccca tctctgagga ttacagcgtc gccagcgcag ctctctctag cgacggccgc
     4981 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     5041 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     5101 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5161 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5221 gaattgctgc cctctggtta tgtgtgggag ggctgacctg caggataact tcgtatagca
     5281 tacattatac gaagttatca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     5341 aaaaagtggc accgagtcgg tgcttttttg ttttagcata acttcgtata atgtgtacta
     5401 tacgaagtta tctagaggta cccgttaaga tcggaagagc acacgtctga actccagtca
     5461 caaaattatc tcgtatgccg tcttatgctt gttaattaac aattgacgcg taattctacc
     5521 ggctagcgag agcacacacg aaaggtaatt cgatatcaag cttatcggta atcaacctct
     5581 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5641 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5701 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5761 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5821 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5881 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5941 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6001 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6061 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6121 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     6181 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6241 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6361 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6421 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6481 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6541 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6601 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6661 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6841 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     6901 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6961 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7021 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7081 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7141 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7201 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7261 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7321 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7381 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7441 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7501 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7561 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7621 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7681 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7741 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7801 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7861 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7921 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7981 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     8041 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     8101 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     8161 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     8221 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     8281 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     8341 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8401 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8461 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8521 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8581 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8641 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8701 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8761 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8821 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8881 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9541 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9661 ggtggttttt ttgtttgcaa gcagcagatt acgcg
//
