LOCUS       sequence-441238-        7364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TASL in mammalian cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pMSCV-TASL
FEATURES             Location/Qualifiers
     source          1..7364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(233..252)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(380..402)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(596..615)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     715..737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(775..793)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        861..965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(966..1034,1035..1826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1184..1203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1997..2585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2486..2505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2739..2756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2873..2894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2909..2914,2915..2932,2933..2939)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2947..2963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3125..3639
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(3701..4414,4415..4417,4418..4420))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3746..3767)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4095..4114
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4354..4375
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4432..4984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4434..5000
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4594..4613)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4823..4840
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5948..5971)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5971..5980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5993..6409)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6009..6025)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6041..6063)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6476..6817
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(6881..7364,1..33)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 agcttgccaa acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa
       61 atcttttatt ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc
      121 aaaactagag cctggaccac tgatatcctg tctttaacaa attggactaa tcgcctctac
      181 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      241 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      301 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      361 gcaccattcc ttgcgccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2941 gtggaattgt gagcggataa caatttcaca attctcccca gctctttctg cgctgtccat
     3001 aatgaactat ttcaggagta ttggagtatg tggattcaaa acagtggcta attgagtcag
     3061 gattacttaa tattcgactc ctaccctcag aatgttgttc tccaacttca agaaactgtt
     3121 acccatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa
     3181 cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg
     3241 gcgacgcagt ctatcggagg actggcgcgc cgagtgaggg gttgtgggct cttttattga
     3301 gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat
     3361 aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga
     3421 aactgctgag ggcgggaccg catctgggga ccatctgttc ttggccctga gccggggcag
     3481 gaactgctta ccacagatat cctgtttggc ccatattctg ctgtctctct gttcctaacc
     3541 ttgatctgaa cttctctatt ctcagttatg tattttccat gccttgcaaa atggcgttac
     3601 ttaagctagc ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact
     3661 aaataaaatc ttttatttta tcgaattctt aattaattca ttacttgtac agctcgtcca
     3721 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     3781 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     3841 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     3901 cctcgatgtt gtggcggatc ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcca
     3961 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     4021 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     4081 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     4141 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     4201 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     4261 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     4321 cctcgccaga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     4381 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggttgtggcc atattatcat
     4441 cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac
     4501 ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg
     4561 ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact
     4621 ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta
     4681 tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc
     4741 ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc
     4801 ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg
     4861 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
     4921 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
     4981 acgttagggg ggggggcgga attcgatatc aagcttatcg ataccgtcga cgtttaaacg
     5041 cggccgctta tcatggggtg atgttgctat actgtgaaat gtggagacta ggagtgctga
     5101 tttcgctgga taccagttgc aacaagtggg agatcacaat atctttaact tttgaggtgt
     5161 caagattctt ctccttggac acctgaagag agatttggtc cacgttggtc ataataaatt
     5221 cggatgcgag aatttgtgta ggactagagt cgtggaaaac tgtgtccatg atgtactgtt
     5281 tatacagttc aacaaccttt tgttcgaggt attcattgag tacagcgtta ctaataggtt
     5341 tctgcatctg gaggcttgac ttctctttaa tgcttgtgat cctccagtat gaggaatacc
     5401 tctggatggg tttcaaaggg agttctgtaa gctgggtaga tgtgctgtcc tccattgaga
     5461 gactgatttc tgaactcttc agaagtgggc catcctcaaa tgaggactcg gaaggaaaat
     5521 tagccatgac agagtttatt gccattactt ggccaccggc aatgtgcaaa tcattataat
     5581 tcttacaaat actcttacag ctgggtggca ccaggtatgt ctctcgtata acgtcgcggc
     5641 agatgccaac ggctgcgaga gttggggact caaagacagg gttgctgtta gcttgaagta
     5701 cggttcgaga ttgacttcgg gagtgctggg aagctctggc gtgcacgctg cttataaact
     5761 tcccggagga cttacaactc acatacaggg actggacctg agtctcgtcc acactgctat
     5821 aagagagggc agcgacggag cttgtctcct tcccctggtc tcctgcaact ggttcgttat
     5881 aagatgcgca gttccagtgg atgtcattcc agtaagtgag gccagagaga tagccttctg
     5941 agagcatctt atcatcgtcg tctttataat ccatggtggc agatctaatt ccggcgccta
     6001 gagaaggagt gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa
     6061 gggggagaga cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct
     6121 tgacccaggg aggggggtca aaagccaagg cttcccaggt cacgatgtag gggacctggt
     6181 ctgggtgtcc atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc
     6241 ggttaaaggt gccgtctcgc ggccatccga cgttaaaggt tggccattct gcagagcaga
     6301 aggtaaccca acgtctcttc ttgacatcta ccgactggtt gtgagcgatc cgctcgacat
     6361 ctttccagtg acctaaggtc aaacttaagg gagtggtaac agtctggccc taattttcag
     6421 acaaatacag aaacacagtc agacagagac aacacagaac gatgctgcag cgctgcagca
     6481 gacaagacgc gcggcttcgg ttccaaaccg aaagcaaaaa ttcagacgga ggcgggaact
     6541 gttttaggtt ctcgtctcct accagaacca catatcctga cggggtcgga ttccacatcg
     6601 actcccttcc tcaggtcggg ccacaaaaac ggcccccaaa gtccctggga cgtctcccag
     6661 ggttgcggcc gggtgttcag aactcgtcag ttccaccacg ggtccgccag atacagagct
     6721 agttagctaa ctagtacaga cgcaggcgca aacattagat gccggcacaa acacgcacaa
     6781 agacagagac agacacgaaa cgaccgctgg ccagcttacc tcccggcggg ggggtcggtg
     6841 gtccctgggc aggggtctcc aaatctcggt ggaacctcca aatgaaagac ccccgaggtg
     6901 ggcagtcaat caatctgagg agaccctccc aaggatcagc gagtccacga ttcggatgca
     6961 aacagcaaga ggctttattg ggaatacggg tacccgggcg acgcagtcta tcggaggact
     7021 ggcgcgccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
     7081 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
     7141 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc gggaccgcat
     7201 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     7261 gtttggccca tattctgctg tctctctgtt cctaaccttg atctgaactt ctctattctc
     7321 agttatgtat tttccatgcc ttgcaaaatg gcgttactta agct
//
