LOCUS       sequence-441243-       13694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xla.Tubb2 promoter driving expression of human glucagon receptor, 
            TEV protease recognition site, QF fusion protein for use in 
            trans-Tango..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkac A, 
            Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, 
            Halpern ME
  TITLE     Transsynaptic labeling and transcriptional control of zebrafish 
            neural circuits.
  JOURNAL   Nat Neurosci. 2025 Jan;28(1):189-200. doi: 
            10.1038/s41593-024-01815-z. Epub 2024 Dec 19.
  PUBMED    39702668
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-024-01815-z"; journalName: "Nat Neurosci"; date: 
            "2025-01"; volume: "28"; issue: "1"; pages: "189-200"
COMMENT     Sequence Label: Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP
FEATURES             Location/Qualifiers
     source          1..13694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    475..609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    609..633
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3113..3133)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(4577..4601)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8088..8108)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8128..8144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(8517..8679,8680..8691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     9040..9061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9224..9328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9329..9397,9398..10189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9547..9566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10360..10948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10849..10868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11102..11119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11236..11257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11272..11277,11278..11295,11296..11302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11310..11326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11315..11337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11334..11350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11369..11389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11371..11389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     11426..11442
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11426..11442
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(11476..11487,11488..11675)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    12114..12248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12264..12280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12288..12308
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    12309..12534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12523..12540
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(12903..13616,13617..13619,13620..13622))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12948..12969)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     13297..13316
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13556..13577
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      13619..13628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cacaggcttt gttcttctga tacaacacag catggtggcg gccctagggt tgcctcagtg
       61 tgtagagatg ttcaggatgc tcctttgcca aggtctgatg tctgtgagat gatctgtgcg
      121 agctttatat tcacccgagt aggtgggtct acaatgggat tatggttagg gtcttagttt
      181 tcagggacta atgtgtttaa ctaaaaggag tgcacaatct gttaaatcca aaagtttaca
      241 gagtcaaggg aagtgcctac tctatgtttg tttgactaat ctatccatca agtgaataga
      301 ctttttattt tgttttttct acattgaaaa ttctaaatcg atttgcatca accctatctt
      361 tacttcagct ctatagatgc atcagtggct ctagctatgc ctggagtggt tgaaaaaaac
      421 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      601 gtctggatcc cactttgtac aagaaagctg ggttggcttt agtcgacggt atcgatagac
      661 gtcactattg ctcatacgtg ttgatatcgc taaaattaca ccctgggaca aacccgaggt
      721 tcgtcatgaa ctgcggatca acgtcaaccg catccgtgta ctcgatcgat gccagatcat
      781 ccaccatcgc atcatagtct acagtcgaat atccgaaatt ggccatgggg acgaggttac
      841 tatggtaagg aggatggttc gttgtactat caacgtatgg cgggggtggt ctgggaggag
      901 cacctccagg cgcaagggcc accgaggcct cgttcaagtg ggtggcaggt ggtggatatg
      961 tactgagaat gccatgggta ttgactgatc cgtagtcatt ggtttgagta attgcgtcta
     1021 gggccgcatc gctattgtag ggggtaggat tcggtattcc attaaaccga tcattgaccg
     1081 gatttattga cgttgacagg ggctgttcca tgacatgggg catgatactt gcagtggcag
     1141 tcgcagtcgg cggaagctca agttgtcgta tgggttggag gccagtattg ctgtgtcggc
     1201 ctccccgagg ccaaatggag gagtaggcgg ggaaaagctc ttccaagtgc cttcgatgcc
     1261 gctctgaaga atgtgattgt ttctttaaag caagaaggga gtctagaaga gaagggatca
     1321 gaggtgggat tgcatatgta ccaaaccgct tctgatactg atcaagagtt tcgatcagaa
     1381 gatgcgagta gtgttcgttg agaggctcag aaaaggcagc tgcccataaa taaacggtac
     1441 gggtaaggta tgcagtaggc acaaaagccg atgctgtatc ctgggaaagg acaaagacgc
     1501 tgaagggaga attgtggccg attacctgct gcaaccgcga cctgaactcc aagggagcgt
     1561 tctgtgaaga tgttcgtatt cttggcagaa gctccaaagc aaccagcttg gtcacgccat
     1621 ataggtggtt gaatgtgctt aaagggtatg ccgggttcct caacatttgc agcatggtgt
     1681 gttcaccgcc taagccctca catggtgtcc acggctccca ttgatcttga ccgtcttcag
     1741 gtaacggcag atccgcaatg tcgtctggct tgagatgggc cggcacgttg tgtctcatag
     1801 acacaatggt atccacgatg aagcacgcga cgagcagcgc acgcattctt ggtcgtggcc
     1861 ggtcatcatc aggaggcaac tgggtgcgaa caacaagtag gacgcggacc gcaaagccaa
     1921 ccagcaacca agcagactcc caatcatccc gcccaaggct tacgagacaa agaagcagca
     1981 acgctctgca gtgctgaacc tggaacggcc cgctttccaa aggtatgagc tttcgggctg
     2041 tgtcagatat gtctgcaggt gaaggattga tggcattgtc atggccatga gcagctgaag
     2101 ctggacccgt agcactcgat gcagcagtag cagcagcctg gaaggacgcc agggcgagcg
     2161 cgctccagag ctcggcatga acgccagact cgacattcgc agagggaagc aacgagcctt
     2221 gttcagagta ctggtacaat gcttggtaca tgtctttctt ctcgaggata gggagccaag
     2281 aatgcgtgta ggaaaagtag atatccagca ggcgccaatg attgggtggt agcttcccta
     2341 tcccggtacc atctggctga aggttgtttt ccagggtgtt tgccgaaata ggtggtcgga
     2401 ccgggcctgg tagagtgcgt gtctcagctg tactagactt aggcgccgag aaagtcaagt
     2461 gaggcgcatg agggaagtcg gatgtctttg ccccggcatc ttggacattt atgtcttcgg
     2521 acggggattg gccgtcttca gatggatctt gtgcggcctg acctgagaga agacgggtga
     2581 tgcttttgtt gactcgactc gtcgcccatc ttgcatgcag gcgttctgca gcaggcgagt
     2641 ctttgccgac caggagagcg ctgccctggc cagcatcacg gaccaaaaga ttgtggaggg
     2701 cgtcctcgct gcgggcaacg ttctcgaaca tccaagccag agccagttcg agagtgcgga
     2761 tgtagcccgt ctggactcct cgcttcttgg gactggcctg gtaggtacac gaccggccct
     2821 gcgacacaca ggggaagcaa gccggctgga ttccatcaca cttttcacgt gcagctcgac
     2881 actgatcaca ggccctcgag actctctggc gtttcgaacg agcgggctcg acaagtaacg
     2941 tcgtcgtggt agggccgagg ccgggtgtgt caatgttggt caagtccgca aaacgggcgt
     3001 tgttgctgct tgctgcagtg ttagcgacat gaccatcagc attgccatca gcatggccat
     3061 cagcgtgggc attggcttcg gcagcggcat tgagtgtctt gcgtttaggc gggctctgga
     3121 agtacaggtt ctcggatccg aaaggagact cagccagtct tggcaggcct ccagccagag
     3181 gggtctcagc gctagagtcc tgagatcctc ctcctcttcc aaactggagt tccttgctag
     3241 gaggtccgtg tccaggagaa gaagaagctc tgtggttgct agtatttctc tcctcccaca
     3301 gcacctttcc cagtctccat ctgtgccatc ttcttctgag ctcagactgc acctccttgt
     3361 tcagaaagca gtacagcaca gccaccagca gtccctggaa agaagacagg aacaggtcaa
     3421 agaacagctt ggcagagcgc agggttccct gggcatgctc atcagtgaca aaagcaaaca
     3481 ccacctcatg cactcccagc agagggatca gggtcagggt gctcttggcc agtctgaact
     3541 tgtagtcagt gtgatgcatc tgtctggctc tcagcttggc caccagcagc tgcacaattc
     3601 tcacaaagat gaagaagttg atcaggatgg ccaggaacac agggaatctg agaatccacc
     3661 agaatcccat gttgtcattg ctagtccaac actgcacatt ctcaaacagg cacttcacca
     3721 cagcccaagg gaccacaaac agcattggag cgccccatcc aattcccagg tacagagaga
     3781 agaaagagcg ctcaggcagg gtggccagtc ccagcagatt gtgcaggtac agtccctcca
     3841 ccagcagcca gcagtagttg gccacaattc catattgcat gaacacagca gccactctgc
     3901 agccagccac agctccatca gacagccagg tgctcacaga caggtcatct ccaatcttct
     3961 gagagtatct ggttctgagc agtccatcaa tcaccagcac agaagaagcc ttcagcacaa
     4021 aagaagcaaa caggttggca tggatggcat ttctggtaca atggagcttg ctcagtcctc
     4081 ccaggatggc cagagccagc agcagagcgc ccagagacag agagtatccc acagtgtaca
     4141 tcacctggaa agaagagtac atcttggcca cctccttctg cacctcaatc tcctctccat
     4201 ccatctggca ctgagaagca tctctccaag gctgtcctct tgggcctctc acccactgtc
     4261 catcaggtcc gcatctcttg aacacaaatc tgtgctgcac cttgtggtgc caaggcagat
     4321 accaagggca agagatgttg gcggtagtat tggcaggggt gtcaggccag caagagtact
     4381 tgtcaaaggt tctgttgcac accagctcag ttggaggagg cagcagagac aggttgtggt
     4441 ggcactggtc tccatacagc ttccacttct caaacaggaa gtccatcacc tgagcagaag
     4501 ggacctgagg ctgacaagcc agcagcagca gcagcagcag cagaggtctc tgaggctgac
     4561 aaggtggcat ggtggcagcc tgcttttttg tacaaacttg aagcttgatt gggttgagtc
     4621 ttacagtggt gtaatcagtg gatggtgtgg ccctttcttg ttgctttact tgccctttgt
     4681 gcaacacccc gatggtttcc agccggctaa gaatctgggc tgtcagaggc tccttctgct
     4741 gccttttata cacttgtgac gccttgggga gggtgctgct ctctcctatt cacttgctgt
     4801 ggtcctgggc tcagatgatg taatggtggg tggagccact ggccctgagc attgtaggga
     4861 tgggaaccaa tcaaatgaca agggagggaa tggaaatgga tgctgcattg tctcaccatc
     4921 aggcagatac caccaagaag gtgaaaaaga tgctgggaat gtgcttattg cttattacaa
     4981 aggaatatta tactgaaata atgaaaaaaa attctaatta aaacacgaat gagaagctga
     5041 ttaagcctat agaaacatta accttgttat tgataatatt agcctaatga tgactccaaa
     5101 ggtattagtg acaggtacca tcccccacac aactcgtcca tcactgggag ctgtaggggg
     5161 aaggaaggtg cagtggggat gggtgccacc ctatgcagct gcctcacaat ttcattttat
     5221 cacatgatac tgctccatat ccatttctgc ctcaaatcac tctccagggg attcccgagt
     5281 cagtgtttca atatcccccc tgtattcaaa tggaagcagc acatctgcct cagaccgcaa
     5341 acacacacca gttttacagg gcaggaaatc atatggccct taatttattt aattgcttcc
     5401 gtaaagacca gtggatgtag cagaagaacg aggaggttga acaaacgggg gcagttaaat
     5461 ggttttactg acatctgtcc aaacatacag aacaaaaatg tgaaataaaa tcacaatcac
     5521 aatttacata tgagtagaca gggctgaaac cttgcctatt aaaagtggca aagctagggg
     5581 aaaccagtgc tattggatta acctttaccc tggaacctcc catccaaatg tacattaagg
     5641 ttatgcccaa actagaggcc catatgcttt acatagtcca tcttctttct agttacaccc
     5701 acatagaaat acttgtaagt gcccgttggt agaagctaag catcagtaaa tacatctgca
     5761 gtggaacaca agggggatcc tgggagtaat cagctgatag ctagaaaatg cagcaaaatg
     5821 tgtaaaatgc tgctgagcat tagaatcagc atgacagcca gggttccatt ctttctgtag
     5881 cactgatgtg ctgataaaag ctgccagagc agcatagtga ttgcaaatga gaaatattaa
     5941 tagccaataa atcagtgtaa tttccttatc atgtcattac aatatatcac atttatgcca
     6001 cttttgtttg gattgtgtat tatttctata gagagaaaac acaaccaata ttaaatatta
     6061 caaatatttt tatcatactc acatatactg tacctaataa gctggcagac atcatatgaa
     6121 tttaggggtt tggagaaaaa tgattgaatc agtacatcct tatgggtggc aatacatggc
     6181 caagtataac tgctgactca gctcttgcag acataatagt gtttaaaatt gtgtcatact
     6241 cacatatacc taataagcac gcagactcgg tacatcctta taggtggtga ttgataaatg
     6301 gccaagtata agctgccaac tcagctcttc cagaattagt tggcagttat ggttaccacc
     6361 ttttctggga aaaaagtaac agccttctta tattttaatg tttttttcca attattgaca
     6421 ttggcatcaa gcaacatttt ttctggccag gctagtaaaa tatccacaag taggcaaccc
     6481 aatcagcagc ttattggccc atgcatgagg ccttctcaat ggcctgcctg accaatattt
     6541 gactatcaag cagatatatc tatcagacag tttggataat catgttggaa gaggaccaca
     6601 ttggcaaatg gctcccaaat atgatctgat tgttagcctg ttgatcagat catcctgatt
     6661 tggcccagtg tgttgatgtt gaatattgtt tggctatgcc caccttaagg aaaatgtttg
     6721 catttcaaga ttactatgaa ggagcattaa atgattaaaa aatacagaaa gcaaagctga
     6781 tatcactgaa agtgggcaac acccaagatt tacaatatat tctcctggta ttacactatg
     6841 agcatacagg agtctgttcc ttactgcaat gttctgaagg taatgtaatc actgttgtta
     6901 atgttaaaat gttagtaaca gatgtactac caataaatat ggatttcagt ggtacagctg
     6961 tgtgggaaga ggtcctcgag acagacattc ttactgtcag tcaggcagcc ttttgcagat
     7021 atgcagacac agctttctcc cttcttcctt gtgctgcata aggggagggg tagggcagag
     7081 cagaattcac acaaagcatg aggagatgct aatgaaagtg tcacatggca tgggggggga
     7141 gaagatgctg catttggaag aggagactca cacaccagga acatggacat tactctgtgt
     7201 ggagtgaagg gaacacattg ttttgaaagg atgagaaaag caggacctgg caaagctcat
     7261 aaaatagtct gtgctcagta accaagcaaa ctcatgacag caaatggaca ccattaacca
     7321 ggttctcaca tcatacctac atgtgtataa gtccataaaa gccatgcttc taaaatatca
     7381 aattatattc tgtcttgtca agacaccata ttgcttatcc atggcatata tcgaattatt
     7441 tattctgcag gcagatatta tccttaaaat aattattcag tttaaaaata aaaactgggt
     7501 aaatactgta gattggctat gcaaaataaa aaatgtttct aatatagtta gttagccaaa
     7561 aatgtaatgt ataaagactg gagtgacata agtagaacac tacttcctgc tttgcaggtc
     7621 tcttgatttc cactgattgg ttacaaggtt acaaggcaat aatcagagac ttgagggggg
     7681 ctgtaaggca gggtgtccca aaatcagaac agacaccaag gacccagtcg cggctcaccc
     7741 ttctgcctat aacagccgcc tttggcatcg ggactagccc tctgctactc agatgacgcc
     7801 aggtcttaac gtgagaggcc caaggagaga gttctgacca agcaagggaa ccaagtgtgg
     7861 ggttgtagaa ttggaaacaa gaattgtagt taaaagccag gccgggtcag taacaatcag
     7921 tcaatgcggt acagttacag gagacaaata aatggttggg gtcacaggcc gagtcaaaca
     7981 caccaaaata gaaagtacac cacaggatac aagagagtag tcaaaaaaca ggcaataatc
     8041 cggacaggca gcaaactaga tgtaatcagg aacagacagg gtctagacaa cttttctata
     8101 caaagttgat agcttggcgt aatcatggtc atagctgttt cctgctcgag ggcccatctg
     8161 gcctgtgttt cagacaccag ggagtctctg ctcacgtttc ctgctatttg cagcctctct
     8221 atcaagacta atacacctct tcccgcatcg gctgcctgtg agaggctttt cagcactgca
     8281 ggattgcttt tcagccccaa aagagctagg cttgacacta acaattttga gaatcagctt
     8341 ctactgaagt taaatctgag gttttacaac tttgagtagc gtgtactggc attagattgt
     8401 ctgtcttata gtttgataat taaatacaaa cagttctaaa gcaggataaa accttgtatg
     8461 catttcattt aatgtttttt gagattaaaa gcttaaacaa gaatctctag ttttctttct
     8521 tgcttttact tttacttcct taatactcaa gtacaatttt aatggagtac ttttttactt
     8581 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     8641 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gtcaagaact
     8701 cctggacaaa cctctgacct gtgtggaaca gagtggatat gggtgtctga acagatattc
     8761 acgtcttttg cagatcagag ggcatttctg gtgttgtgca atgacctggg tccaacaggt
     8821 aggcgtactc gtatccaaga gcattgttgg acaggaggta gtctccattc ctataaagag
     8881 gaaggaatgg caccatgtag tagccgtcat tgtggccaat tggtgcattg gtacgtgggt
     8941 agatggaccg gggaggctga tgagttctta gccagcgtgg gccatcgccc tgatagacgg
     9001 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9061 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9121 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9181 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10141 aaatagacag atcgctgaga taggtgcctc acttattaag cattggtaac tgtcagacca
    10201 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10261 ggtgaagatc ctttttgata atctcatgac caaaatgcct taacgtgagt tttcgttcca
    10321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10501 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11221 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11281 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11341 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    11401 ggagctccac cgcggtggcg gccgctctag aactagtgga tctgctgggc ttgctgaagg
    11461 tagggggtca agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt
    11521 aagtattatt tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac
    11581 ttttacttaa ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt
    11641 acagtcaaaa agtacttatt ttttggagat cacttcattc tattttccct tgctattacc
    11701 aaaccaattg aattgcgctg atgcccagtt taatttaaat gttatttatt ctgcctatga
    11761 aaatcgtttt cacattatat gaaattggtc agacatgttc attggtcctt tggaagtgac
    11821 gtcatgtcac atctattacc acaatgcaca gcaccttgac ctggaaatta gggaaattat
    11881 aacagtcaat cagtggaaga aaatggagga agtatgtgat tcatcagcag ctgcgagcag
    11941 cacagtccaa aatcagccac aggatcaaga gcacccgtgg ccgtatcttc gcagatctga
    12001 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    12061 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    12121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12241 tctggatcat catcgatggt accgtaaaac gacggccagt gaattatcaa ctatgtataa
    12301 taaagttgcc atagagccca ccgcatcccc agcatgcctt gctattgtct tcccaatcct
    12361 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
    12421 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
    12481 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
    12541 ctgatcagcg ggtttcgggc cccccctcgt gccctacagg ttgtcttccc aacttgcccc
    12601 ttgctccata ccacccccct ccaccccata atattataga aggacaccta gtcagacaaa
    12661 atgatgcaac ttaattttat taggacaagg ctggtgggca ctggagtggc aacttccagg
    12721 gccaggagag gcactgggga ggggtcacag ggatgccacc caattcctgc agaattcgat
    12781 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
    12841 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg
    12901 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
    12961 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
    13021 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
    13081 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg
    13141 ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgatgtagtt gtactccagc
    13201 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
    13261 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
    13321 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
    13381 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cccaggtcag ggtggtcacg
    13441 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
    13501 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
    13561 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
    13621 atggtggcgg ccggtggatc ccgggcccgc ggtaccgtgc tctgcagttc ctggtcagcc
    13681 tcctcattct tctc
//
