LOCUS       sequence-441247-        4951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for an inducible V5-tagged CXADR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4951)
  TITLE     Janes lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEN_TT CXADR-V5
FEATURES             Location/Qualifiers
     source          1..4951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(32..56)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             58..99
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(138..237)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(349..368)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(427..446)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1020..1553)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1742..1747,1748..1764,1765..1770))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2427..3015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2916..2935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3180..3266
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3358..3385
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3393..3416
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    3435..3534
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3566..3880
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3574..3592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3610..3628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3645..3663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3681..3699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3717..3735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3752..3770
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3788..3806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3853..3877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ccagcacaga gcaaggatgg gtctatagta tacccaactt tcttgtacaa agtggttggt
       61 aagcctatcc ctaaccctct cctcggtctc gattctacgt agcaattgga agactaatgc
      121 gttttcgaga tatctagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
      181 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
      241 tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      301 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      361 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      421 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      481 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      541 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
      601 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
      661 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      721 cgctggcagt gtccctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
      781 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
      841 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
      901 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
      961 accttatttt tgacgagggg aaattaggcc gggaagccga tctcggcttg aacgaattgt
     1021 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
     1081 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
     1141 ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc
     1201 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
     1261 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
     1321 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
     1381 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
     1441 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
     1501 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct
     1561 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     1621 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     1681 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     1741 cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg
     1801 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc
     1861 gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt
     1921 ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc
     1981 gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct
     2041 ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctact
     2101 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2161 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2221 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2281 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2341 cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2401 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2461 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2521 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2581 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2641 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2701 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2761 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2821 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2881 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2941 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3001 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3061 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3121 gctagcatgg atctcgggga cgtctaacta ctaagcgaga gtagggaact gccaggcatc
     3181 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
     3241 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
     3301 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga
     3361 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctgtt agttagttac
     3421 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     3481 aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagca ggctttaaag
     3541 gaaccaattc agtcgactgg atcccgagtt tactccctat cagtgataga gaacgtatgt
     3601 cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag
     3661 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
     3721 tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg
     3781 agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc
     3841 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc actagtatgg cgctcctgct
     3901 gtgcttcgtg ctcctgtgcg gagtagtgga tttcgccaga agtttgagta tcactactcc
     3961 tgaagagatg attgaaaaag ccaaagggga aactgcctat ctgccgtgca aatttacgct
     4021 tagtcccgaa gaccagggac cgctggacat cgagtggctg atatcaccag ctgataatca
     4081 gaaggtggat caagtgatta ttttatattc tggagacaaa atttatgatg actactatcc
     4141 agatctgaaa ggccgagtac attttacgag taatgatctc aaatctggtg atgcatcaat
     4201 aaatgtaacg aatttacaac tgtcagatat tggcacatat cagtgcaaag tgaaaaaagc
     4261 tcctggtgtt gcaaataaga agattcatct ggtagttctt gttaagcctt caggtgcgag
     4321 atgttacgtt gatggatctg aagaaattgg aagtgacttt aagataaaat gtgaaccaaa
     4381 agaaggttca cttccattac agtatgagtg gcaaaaattg tctgactcac agaaaatgcc
     4441 cacttcatgg ttagcagaaa tgacttcatc tgttatatct gtaaaaaatg cctcttctga
     4501 gtactctggg acatacagct gtacagtcag aaacagagtg ggctctgatc agtgcctgtt
     4561 gcgtctaaac gttgtccctc cttcaaataa agctggacta attgcaggag ccattatagg
     4621 aactttgctt gctctagcgc tcattggtct tatcatcttt tgctgtcgta aaaagcgcag
     4681 agaagaaaaa tatgaaaagg aagttcatca cgatatcagg gaagatgtgc cacctccaaa
     4741 gagccgtacg tccactgcca gaagctacat cggcagtaat cattcatccc tggggtccat
     4801 gtctccttcc aacatggaag gatattccaa gactcagtat aaccaagtac caagtgaaga
     4861 ctttgaacgc actcctcaga gtccgactct cccacctgct aaggtagctg cccctaatct
     4921 aagtcgaatg ggtgcgattc ctgtgatgat t
//
