LOCUS       sequence-441281-        8605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-color splicing reporter expressing eGFP and tdTomato from the 
            CMV early enhancer/chicken ß-actin (CAGGS) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu
            Q, Zhang C
  TITLE     CRISPR-dCas13d-based deep screening of proximal and distal 
            splicing-regulatory elements.
  JOURNAL   Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
  PUBMED    38714659
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-7"; volume: "15(1):3839. doi"; pages: " 
            10.1038/s41467-024-47140-8"
COMMENT     Sequence Label: pTomatoSlicer
FEATURES             Location/Qualifiers
     source          1..8605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        565..944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        975..1079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1080..1148,1149..1940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1298..1317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2111..2699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2600..2619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2853..2870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2962..2981)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3016..3035
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3078..3273)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3092..3227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    3332..3353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3368..3373,3374..3391,3392..3398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3406..3422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3430..3446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3789..3808
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3807..3862
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3908..3927
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     4029..4045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4064..4084
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4066..4084
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    4180..4291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4280..4297
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4324..4912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4430..4441
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4839..4859
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(4919..4938)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4921..5053
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(5076..6503)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     misc_feature    join(6513..6521,6522..6524,6525..7099)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6686..6705)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6915..6932
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(7135..7156)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(7291..7803,7804..7806,7807..7809))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     7484..7503
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7743..7764
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7843..7862)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          join(7870..8605,1..281)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7924..7946)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg
       61 ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa
      121 gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc
      181 ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg
      241 gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc
      301 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
      361 ctctgattgg ctgccgccgc acctctccgc ctcgcccccc cccccccccc cccccccccc
      421 cccccgcccc gccggccgcc cccccccccc cccccccgcc cccatcgctg cacaaaataa
      481 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
      541 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
      601 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
      661 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
      721 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
      781 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
      841 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
      901 ccccgtaatt gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt
      961 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1021 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1081 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1141 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1201 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1261 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1321 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1381 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1441 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1501 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1561 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1621 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1681 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1741 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1801 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1861 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1921 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2761 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2821 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2881 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat
     2941 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3001 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3061 aataaacaag ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     3121 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     3181 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     3241 ggcgggacta tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg
     3301 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3361 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3421 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg
     3481 gatctccata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
     3541 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
     3601 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
     3661 gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc
     3721 agcatatggg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     3781 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     3841 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     3901 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
     3961 acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgaatta gcggataaca
     4021 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     4081 aagggaacaa aagctgggct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
     4141 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     4201 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     4261 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcgtaccga gctcgaattc
     4321 caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     4381 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     4441 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     4501 atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     4561 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     4621 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     4681 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     4741 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     4801 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     4861 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgacac
     4921 ctgtggagag aaaggcaaag tggatgtcag taagaccaat aggtgcctat cagaaacgca
     4981 agagtcttct ctgtctcgac aagcccagtt tctattggtc tccttaaacc tgtcttgtaa
     5041 ccttgatact tacctgcggc ctcgagttac ttaagctact tgtacagctc gtccatgccg
     5101 tacaggaaca ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc
     5161 tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt agtagccggg cagttgcacg
     5221 ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt agtggccgcc gtccttcagc
     5281 ttcagggcct ggtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg
     5341 gaggcctccc agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg
     5401 ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg
     5461 gtcaccagac cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag
     5521 gacagcttct tgtaatcggg gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac
     5581 atgaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc
     5641 agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac
     5701 tcgtggccgt tcatggagcc ctccatgcgc accttgaagc gcatgaactc tttgatgacg
     5761 gccatgttgt tgtcctcgga ggaggcggtg ccggagctgc cgctgccggt gctgccggtg
     5821 ccatgcccca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc cacgatggtg
     5881 tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta gccgggcagt
     5941 tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc
     6001 ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc
     6061 tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc gggggggaag
     6121 ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg
     6181 gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
     6241 gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta cgccttggag
     6301 ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc
     6361 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
     6421 tcgaactcgt ggccgttcat ggagccctcc atgcgcacct tgaagcgcat gaactctttg
     6481 atgacctcct cgcccttgct cacgctagcc atggttgtgg ccatattatc atcgtgtttt
     6541 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     6601 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     6661 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     6721 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     6781 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     6841 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     6901 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     6961 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     7021 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     7081 ggggggggag ggagaggggc ggaatttcta gatccggtgg atcccggcgg cggtcacgaa
     7141 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     7201 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     7261 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     7321 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     7381 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     7441 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     7501 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     7561 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     7621 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     7681 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     7741 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     7801 gctcaccatg gtaccgaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt
     7861 gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
     7921 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc
     7981 cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg
     8041 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct
     8101 agagggggtg cggcggcgcc tcccagattt cggctccgca cagatttggg acaaaggaag
     8161 tccctgcgcc ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg
     8221 acagccgccg gcgctccggg ggccgccgcg cccctccccc gagccctccc cggcccgagg
     8281 cggccccgcc ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc
     8341 cgcgccacgc cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg
     8401 aggggggtgc agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc
     8461 cacgcacaca ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc
     8521 accgcccccg gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg
     8581 cccgcagcgc tcacagccgc cgggc
//
