LOCUS       sequence-441362-       13168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) 
            to generate ACE2(WT)-expressing cells. Codon-optimized for human 
            expression. Insert contains C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Gunnels TF, Stranford DM, Mitrut RE, Kamat NP, Leonard JN
  TITLE     Elucidating Design Principles for Engineering Cell-Derived Vesicles 
            to Inhibit SARS-CoV-2 Infection.
  JOURNAL   Small. 2022 May;18(19):e2200125. doi: 10.1002/smll.202200125. Epub 
            2022 Apr 7.
  PUBMED    35388947
REFERENCE   2  (bases 1 to 13168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small."; 
            date: "2022-05"; volume: "18(19):e2200125. doi"; pages: " 
            10.1002/smll.202200125. Epub 2022 Apr 7"
COMMENT     Sequence Label: pTFG001-ACE2(WT)-HA
FEATURES             Location/Qualifiers
     source          1..13168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2791..3094
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3095..3298
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3249..3269
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5827..5853
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5873..6445
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6040..6057)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6267..6286
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6448..7050
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6947..6967
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7143..7731
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7196..7216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7614..7625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7938..8171
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8272..8289)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8278..8502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8548..8976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8635..8654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8845..8866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8985..9005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8990..9319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9170..9305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9368..10393
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9368..10393
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10523..10644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10693..10709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10693..10709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10706..10728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10717..10733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10741..10747,10748..10765,10766..10771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10786..10807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10924..10941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11095..11683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11175..11194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11854..12645,12646..12714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12477..12496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12715..12819)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12867..13001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3361 acaccgactc tactagagga tctgccacca gctagcgcca ccatgagcag ctccagctgg
     3421 ctgctgctgt ctctggtggc tgttacagcc gctcagagca ccattgagga acaggccaag
     3481 accttcctgg acaagttcaa ccacgaggcc gaggacctgt tctaccagtc tagcctggcc
     3541 tcctggaact acaacaccaa catcaccgaa gagaacgtgc agaacatgaa caacgccggc
     3601 gacaagtgga gcgccttcct gaaagagcag agcacactgg cccagatgta ccctctgcaa
     3661 gagatccaga acctgaccgt gaaactccag ctgcaggccc tccagcagaa tggcagttct
     3721 gtgctgagcg aggacaagag caagcggctg aacaccatcc tgaataccat gtccaccatc
     3781 tacagcaccg gcaaagtgtg caaccccgac aatccccaag agtgcctgct gctggaaccc
     3841 ggcctgaatg agatcatggc caacagcctg gactacaatg agagactgtg ggcctgggag
     3901 agctggcgct ctgaagttgg aaaacagctg cggcccctgt acgaggaata cgtggtgctg
     3961 aagaacgaga tggccagagc caaccactac gaggactacg gcgactattg gagaggcgac
     4021 tacgaagtga atggcgtgga cggctacgac tactccagag gccagctgat cgaggacgtg
     4081 gaacacacct tcgaggaaat caagcctctg tacgagcatc tgcacgccta cgtgcgggcc
     4141 aagctgatga atgcttaccc cagctacatc agccccatcg gctgtctgcc tgctcatctg
     4201 ctgggagaca tgtggggcag attctggacc aacctgtact ccctgacagt gcccttcggc
     4261 cagaaaccta acatcgacgt gaccgacgcc atggtggatc aggcttggga tgcccagcgg
     4321 atcttcaaag aggccgagaa gttcttcgtg tccgtgggcc tgcctaatat gacccaaggc
     4381 ttctgggaga actccatgct gacagacccc ggcaatgtgc agaaagccgt gtgtcatcct
     4441 accgcctggg atctcggcaa gggcgacttc agaatcctga tgtgcaccaa agtgacgatg
     4501 gacgacttcc tgacagccca ccacgagatg ggccacatcc agtacgatat ggcctacgcc
     4561 gctcagccct tcctgctgag aaatggcgcc aatgagggct tccacgaagc cgtgggagag
     4621 atcatgagcc tgtctgccgc cacacctaag cacctgaagt ctatcggact gctgagcccc
     4681 gacttccaag aggacaacga gacagagatc aacttcctgc tcaagcaggc cctgaccatc
     4741 gtgggcacac tgccctttac ctacatgctg gaaaagtggc ggtggatggt ctttaagggc
     4801 gagatcccca aggaccagtg gatgaagaaa tggtgggaga tgaagcgcga gatcgtgggc
     4861 gttgtggaac ctgtgcctca cgacgagaca tactgcgatc ctgccagcct gtttcacgtg
     4921 tccaacgact acagcttcat ccggtactac acccggacac tgtaccagtt ccagtttcaa
     4981 gaggctctgt gccaggccgc caagcacgaa ggacctctgc acaagtgcga catcagcaac
     5041 tctacagagg ccggacagaa actgttcaac atgctgcggc tgggcaagag cgagccttgg
     5101 acactggctc tggaaaatgt cgtgggcgcc aagaatatga acgtgcggcc tctgctgaac
     5161 tacttcgagc ccctgttcac ctggctgaag gaccagaaca agaacagctt cgtcggctgg
     5221 tccaccgatt ggagccctta cgccgaccag agcatcaaag tgcggatcag cctgaaaagc
     5281 gccctgggcg ataaggccta tgagtggaac gacaatgaga tgtacctgtt ccggtccagc
     5341 gtggcctatg ctatgcggca gtactttctg aaagtcaaga accagatgat cctgttcggc
     5401 gaagaggatg tgcgcgtggc caacctgaag cctcggatca gcttcaactt cttcgtgact
     5461 gcccctaaga acgtgtccga catcatcccc agaaccgagg tggaaaaggc catcagaatg
     5521 agcagaagcc ggatcaacga cgccttccgg ctgaacgaca actccctgga attcctgggc
     5581 attcagccca cactgggccc tccaaatcag cctcctgtgt ccatctggct gatcgtgttt
     5641 ggcgtggtca tgggcgtgat cgtcgtgggc atcgtgatcc tgatcttcac cggcatccgc
     5701 gaccggaaga agaagaacaa ggccagaagc ggcgagaacc cctacgccag catcgacatc
     5761 tccaagggcg aaaacaaccc cggcttccag aacaccgacg acgtgcagac aagcttcggc
     5821 accggttatc cctacgacgt gcccgattat gcctgagcgg ccgcaaattc cgcccctctc
     5881 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5941 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6001 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6061 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6121 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6181 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6241 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6301 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6361 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6421 ttttcctttg aaaaacacga taataccatg gccaccgagt acaagcccac ggtgcgcctc
     6481 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6541 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     6601 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6661 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6721 atcggctcgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6781 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6841 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6901 gccgagcgcg ctggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6961 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7021 tggtgcatga cccgcaagcc cggtgcctga gtttgtttga atgaggcttc agtactttac
     7081 agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca ggtttctgga
     7141 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7201 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7261 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7321 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7381 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7441 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7501 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7561 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7621 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7681 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     7741 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7801 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7861 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7921 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7981 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8041 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8101 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8161 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8221 atgaatatca gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg
     8281 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8341 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8401 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8461 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8521 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     8581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8641 tcgctttctt cccttccttt ctcgtcacgt tcgccggctt tccccgtcaa gctctaaatc
     8701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8941 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9001 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9061 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9121 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9181 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9241 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     9301 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     9361 gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     9421 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     9481 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     9541 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     9601 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     9661 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     9721 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     9781 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     9841 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     9901 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     9961 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
    10021 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
    10081 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
    10141 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
    10201 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
    10261 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
    10321 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
    10381 ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    10441 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10501 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    10561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10621 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    10681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12841 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
    12901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12961 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    13021 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    13081 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    13141 gttaaatatg tactacaaac ttagtagt
//
