LOCUS       sequence-441380-        9513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of MAC3-tagged EGFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Liu X, Salokas K, Keskitalo S, Martinez-Botia P, Varjosalo M
  TITLE     Analyzing Protein Interactions by MAC-Tag Approaches.
  JOURNAL   Methods Mol Biol. 2023;2690:281-297. doi: 
            10.1007/978-1-0716-3327-4_24.
  PUBMED    37450155
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2690:281-297. doi"; pages: " 
            10.1007/978-1-0716-3327-4_24"
COMMENT     Sequence Label: MAC3-EGFR
FEATURES             Location/Qualifiers
     source          1..9513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(832..903,904..2766,2767..2835,2836..4461)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPKDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(4463..4487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4534..4557
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4594..4617
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4648..4674
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4648..4674
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5231..5248)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5237..5461
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5594..5613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5745..5792
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5800..6822
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5800..6822
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6952..7073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6989..7008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7043..7062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7122..7138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7135..7157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7146..7162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7170..7176,7177..7194,7195..7200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7215..7236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7353..7370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7524..8112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7604..7623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8283..9074,9075..9143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9144..9248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9323..9342)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catgcgaccc
      841 tccgggacgg ccggggcagc gctcctggcg ctgctggctg cgctctgccc ggcgagtcgg
      901 gctctggagg aaaagaaagt ttgccaaggc acgagtaaca agctcacgca gttgggcact
      961 tttgaagatc attttctcag cctccagagg atgttcaata actgtgaggt ggtccttggg
     1021 aatttggaaa ttacctatgt gcagaggaat tatgatcttt ccttcttaaa gaccatccag
     1081 gaggtggctg gttatgtcct cattgccctc aacacagtgg agcgaattcc tttggaaaac
     1141 ctgcagatca tcagaggaaa tatgtactac gaaaattcct atgccttagc agtcttatct
     1201 aactatgatg caaataaaac cggactgaag gagctgccca tgagaaattt acaggaaatc
     1261 ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt gcaacgtgga gagcatccag
     1321 tggcgggaca tagtcagcag tgactttctc agcaacatgt cgatggactt ccagaaccac
     1381 ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg ggagctgctg gggtgcagga
     1441 gaggagaact gccagaaact gaccaaaatc atctgtgccc agcagtgctc cgggcgctgc
     1501 cgtggcaagt cccccagtga ctgctgccac aaccagtgtg ctgcaggctg cacaggcccc
     1561 cgggagagcg actgcctggt ctgccgcaaa ttccgagacg aagccacgtg caaggacacc
     1621 tgccccccac tcatgctcta caaccccacc acgtaccaga tggatgtgaa ccccgagggc
     1681 aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc gtaattatgt ggtgacagat
     1741 cacggctcgt gcgtccgagc ctgtggggcc gacagctatg agatggagga agacggcgtc
     1801 cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt gtaacggaat aggtattggt
     1861 gaatttaaag actcactctc cataaatgct acgaatatta aacacttcaa aaactgcacc
     1921 tccatcagtg gcgatctcca catcctgccg gtggcattta ggggtgactc cttcacacat
     1981 actcctcctc tggatccaca ggaactggat attctgaaaa ccgtaaagga aatcacaggg
     2041 tttttgctga ttcaggcttg gcctgaaaac aggacggacc tccatgcctt tgagaaccta
     2101 gaaatcatac gcggcaggac caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg
     2161 aacataacat ccttgggatt acgctccctc aaggagataa gtgatggaga tgtgataatt
     2221 tcaggaaaca aaaatttgtg ctatgcaaat acaataaact ggaaaaaact gtttgggacc
     2281 tccggtcaga aaaccaaaat tataagcaac agaggtgaaa acagctgcaa ggccacaggc
     2341 caggtctgcc atgccttgtg ctcccccgag ggctgctggg gcccggagcc caaggactgc
     2401 gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag
     2461 ggtgagccaa gggagtttgt ggagaactct gagtgcatac agtgccaccc agagtgcctg
     2521 cctcaggcca tgaacatcac ctgcacagga cggggaccag acaactgtat ccagtgtgcc
     2581 cactacattg acggccccca ctgcgtcaag acctgcccgg caggagtcat gggagaaaac
     2641 aacaccctgg tctggaagta cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac
     2701 tgcacctacg gatgcacagg gccaggtctt gaaggctgtc caacgaatgg gcctaagatc
     2761 ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg
     2821 atcggcctct tcatgcgaag gcgccacatc gttcggaagc gcacgctgcg gaggctgctg
     2881 caggagaggg agcttgtgga gcctcttaca cccagtggag aagctcccaa ccaagctctc
     2941 ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag tgctgggctc cggtgcgttc
     3001 ggcacggtgt ataagggact ctggatccca gaaggtgaga aagttaaaat tcccgtcgct
     3061 atcaaggaat taagagaagc aacatctccg aaagccaaca aggaaatcct cgatgaagcc
     3121 tacgtgatgg ccagcgtgga caacccccac gtgtgccgcc tgctgggcat ctgcctcacc
     3181 tccaccgtgc agctcatcac gcagctcatg cccttcggct gcctcctgga ctatgtccgg
     3241 gaacacaaag acaatattgg ctcccagtac ctgctcaact ggtgtgtgca gatcgcaaag
     3301 ggcatgaact acttggagga ccgtcgcttg gtgcaccgcg acctggcagc caggaacgta
     3361 ctggtgaaaa caccgcagca tgtcaagatc acagattttg ggctggccaa actgctgggt
     3421 gcggaagaga aagaatacca tgcagaagga ggcaaagtgc ctatcaagtg gatggcattg
     3481 gaatcaattt tacacagaat ctatacccac cagagtgatg tctggagcta cggggtgacc
     3541 gtttgggagt tgatgacctt tggatccaag ccatatgacg gaatccctgc cagcgagatc
     3601 tcctccatcc tggagaaagg agaacgcctc cctcagccac ccatatgtac catcgatgtc
     3661 tacatgatca tggtcaagtg ctggatgata gacgcagata gtcgcccaaa gttccgtgag
     3721 ttgatcatcg aattctccaa aatggcccga gacccccagc gctaccttgt cattcagggg
     3781 gatgaaagaa tgcatttgcc aagtcctaca gactccaact tctaccgtgc cctgatggat
     3841 gaagaagaca tggacgacgt ggtggatgcc gacgagtacc tcatcccaca gcagggcttc
     3901 ttcagcagcc cctccacgtc acggactccc ctcctgagct ctctgagtgc aaccagcaac
     3961 aattccaccg tggcttgcat tgatagaaat gggctgcaaa gctgtcccat caaggaagac
     4021 agcttcttgc agcgatacag ctcagacccc acaggcgcct tgactgagga cagcatagac
     4081 gacaccttcc tcccagtgcc tgaatacata aaccagtccg ttcccaaaag gcccgctggc
     4141 tctgtgcaga atcctgtcta tcacaatcag cctctgaacc ccgcgcccag cagagaccca
     4201 cactaccagg acccccacag cactgcagtg ggcaaccccg agtatctcaa cactgtccag
     4261 cccacctgtg tcaacagcac attcgacagc cctgcccact gggcccagaa aggcagccac
     4321 caaattagcc tggacaaccc tgactaccag caggacttct ttcccaagga agccaagcca
     4381 aatggcatct ttaagggctc cacagctgaa aatgcagaat acctaagggt cgcgccacaa
     4441 agcagtgaat ttattggagc agacccagct ttcttgtaca aagtggttga tatcaaggcc
     4501 gccgacatca ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc
     4561 ggaggatctg gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc
     4621 accgagctgg gcagcaccat ggccagctac ccctacgacg tgcccgacta cgccgacttc
     4681 aagaacctga tctggctgaa ggaggtggac agcacccagg agagactgaa ggagtggaac
     4741 gtgtcctacg gcaccgccct ggtggccgac agacagacca agggcagagg cggcccgggc
     4801 agaaagtggc tgagccagga gggcggcctg tacttcagct tcctgctgaa ccccaaggag
     4861 ttcgagaacc tgctgcagct gcccctggtg ctgggcctga gcgtgagcga ggccctggag
     4921 gagatcaccg agatcccctt cagcctgaag tggcccaacg acgtgtactt ccaggagaag
     4981 aaggtgagcg gcgtgctgtg cgagctgagc aaggacaagc tgatcgtggg catcggcatc
     5041 aacgtgaacc agagagagat ccccgaggag atcaaggaca gagccaccac cctgtacgag
     5101 atcaccggca aggactggga cagaaaggag gtgctgctga aggtgctgaa gagaatcagc
     5161 gagaacctga agaagttcaa ggagaagtga tgactcgagt ctagagggcc cgtttaaacc
     5221 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5281 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5341 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5401 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5461 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5521 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5581 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5641 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5701 ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga
     5761 agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg
     5821 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5881 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5941 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     6001 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     6061 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     6121 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     6181 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     6241 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     6301 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     6361 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     6421 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     6481 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     6541 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     6601 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6661 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     6721 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6781 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc
     6841 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6901 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7081 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7921 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9241 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9301 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9361 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     9421 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     9481 tgcttcgcga tgtacgggcc agatatacgc gtt
//
