LOCUS       sequence-441383-        8346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of MAC3-tagged FGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Liu X, Salokas K, Keskitalo S, Martinez-Botia P, Varjosalo M
  TITLE     Analyzing Protein Interactions by MAC-Tag Approaches.
  JOURNAL   Methods Mol Biol. 2023;2690:281-297. doi: 
            10.1007/978-1-0716-3327-4_24.
  PUBMED    37450155
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2690:281-297. doi"; pages: " 
            10.1007/978-1-0716-3327-4_24"
COMMENT     Sequence Label: MAC3-FGFR1
FEATURES             Location/Qualifiers
     source          1..8346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    585..603
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    606..624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     634..658
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    801..825
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1210..1227)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(3295..3319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3366..3389
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3426..3449
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3480..3506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3480..3506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4063..4080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4069..4293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4426..4445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4577..4624
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4632..5654
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4632..5654
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5784..5905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5821..5840)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5875..5894
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5954..5970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5954..5970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5967..5989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5978..5994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6002..6008,6009..6026,6027..6032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6047..6068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6185..6202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6356..6944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6436..6455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7115..7906,7907..7975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7738..7757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7976..8080)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8155..8174)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8346,1..379)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
      601 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
      661 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      721 ccggactcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg
      781 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccacc atgtggagct
      841 ggaagtgcct cctcttctgg gctgtgctgg tcacagccac actctgcacc gctaggccgt
      901 ccccgacctt gcctgaacaa gcccagccct ggggagcccc tgtggaagtg gagtccttcc
      961 tggtccaccc cggtgacctg ctgcagcttc gctgtcggct gcgggacgat gtgcagagca
     1021 tcaactggct gcgggacggg gtgcagctgg cggaaagcaa ccgcacccgc atcacagggg
     1081 aggaggtgga ggtgcaggac tccgtgcccg cagactccgg cctctatgct tgcgtaacca
     1141 gcagcccctc gggcagtgac accacctact tctccgtcaa tgtttcagat gctctcccct
     1201 cctcggagga tgatgatgat gatgatgact cctcttcaga ggagaaagaa acagataaca
     1261 ccaaaccaaa ccccgtagct ccatattgga catccccaga aaagatggaa aagaaattgc
     1321 atgcagtgcc ggctgccaag acagtgaagt tcaaatgccc ttccagtggg accccaaacc
     1381 ccacactgcg ctggttgaaa aatggcaaag aattcaaacc tgaccacaga attggaggct
     1441 acaaggtccg ttatgccacc gggagcatca taatggactc tgtggtgccc tctgacaagg
     1501 gcaactacac ctgcattgtg gagaatgagt acggcagcat caaccacaca taccagctgg
     1561 atgtcgtgga gcggtcccct caccggccca tcctgcaagc agggttgccc gccaacaaaa
     1621 cagtggccct gggtagcaac gtggagttca tgtgtaaggt gtacagtgac ccgcagccgc
     1681 acatccagtg gctaaagcac atcgaggtga atgggagcaa gattggccca gacaacctgc
     1741 cttatgtcca gatcttgaag actgctggag ttaataccac cgacaaagag atggaggtgc
     1801 ttcacttaag aaatgtctcc tttgaggacg caggggagta tacgtgcttg gcgggtaact
     1861 ctatcggact ctcccatcac tctgcatggt tgaccgttct ggaagccctg gaagagaggc
     1921 cggcagtgat gacctcgccc ctgtacctgg agatcatcat ctattgcaca ggggccttcc
     1981 tcatctcctg catggtgggg tcggtcatcg tctacaagat gaagagtggt accaagaaga
     2041 gtgacttcca cagccagatg gctgtgcaca agctggccaa gagcatccct ctgcgcagac
     2101 aggtaacagt gtctgctgac tccagtgcat ccatgaactc tggggttctt ctggttcggc
     2161 catcacggct ctcctccagt gggactccca tgctagcagg ggtctctgag tatgagcttc
     2221 ccgaagaccc tcgctgggag ctgcctcggg acagactggt cttaggcaaa cccctgggag
     2281 agggctgctt tgggcaggtg gtgttggcag aggctatcgg gctggacaag gacaaaccca
     2341 accgtgtgac caaagtggct gtgaagatgt tgaagtcgga cgcaacagag aaagacttgt
     2401 cagacctgat ctcagaaatg gagatgatga agatgatcgg gaagcataag aatatcatca
     2461 acctgctggg ggcctgcacg caggatggtc ccttgtatgt catcgtggag tatgcctcca
     2521 agggcaacct gcgggagtac ctgcaggccc ggaggccccc agggctggaa tactgctaca
     2581 accccagcca caacccagag gagcagctct cctccaagga cctggtgtcc tgcgcctacc
     2641 aggtggcccg aggcatggag tatctggcct ccaagaagtg catacaccga gacctggcag
     2701 ccaggaatgt cctggtgaca gaggacaatg tgatgaagat agcagacttt ggcctcgcac
     2761 gggacattca ccacatcgac tactataaaa agacaaccaa cggccgactg cctgtgaagt
     2821 ggatggcacc cgaggcatta tttgaccgga tctacaccca ccagagtgat gtgtggtctt
     2881 tcggggtgct cctgtgggag atcttcactc tgggcggctc cccatacccc ggtgtgcctg
     2941 tggaggaact tttcaagctg ctgaaggagg gtcaccgcat ggacaagccc agtaactgca
     3001 ccaacgagct gtacatgatg atgcgggact gctggcatgc agtgccctca cagagaccca
     3061 ccttcaagca gctggtggaa gacctggacc gcatcgtggc cttgacctcc aaccaggagt
     3121 acctggacct gtccatgccc ctggaccagt actcccccag ctttcccgac acccggagct
     3181 ctacgtgctc ctcaggggag gattccgtct tctctcatga gccgctgccc gaggagccct
     3241 gcctgccccg acacccagcc cagcttgcca atggcggact caaacgccgc ttggacccag
     3301 ctttcttgta caaagtggtt gatatcaagg ccgccgacat caccagcctg tacaagaaag
     3361 tggggtggag ccatccccag ttcgagaagg gcggaggatc tggcggcgga agcggcggag
     3421 gaagctggtc ccaccctcag tttgagaaag gcaccgagct gggcagcacc atggccagct
     3481 acccctacga cgtgcccgac tacgccgact tcaagaacct gatctggctg aaggaggtgg
     3541 acagcaccca ggagagactg aaggagtgga acgtgtccta cggcaccgcc ctggtggccg
     3601 acagacagac caagggcaga ggcggcccgg gcagaaagtg gctgagccag gagggcggcc
     3661 tgtacttcag cttcctgctg aaccccaagg agttcgagaa cctgctgcag ctgcccctgg
     3721 tgctgggcct gagcgtgagc gaggccctgg aggagatcac cgagatcccc ttcagcctga
     3781 agtggcccaa cgacgtgtac ttccaggaga agaaggtgag cggcgtgctg tgcgagctga
     3841 gcaaggacaa gctgatcgtg ggcatcggca tcaacgtgaa ccagagagag atccccgagg
     3901 agatcaagga cagagccacc accctgtacg agatcaccgg caaggactgg gacagaaagg
     3961 aggtgctgct gaaggtgctg aagagaatca gcgagaacct gaagaagttc aaggagaagt
     4021 gatgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4261 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4321 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4561 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     4621 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     4681 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     4741 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     4801 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     4861 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     4921 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     4981 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     5041 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     5101 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     5161 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     5221 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     5281 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     5341 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     5401 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     5461 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     5521 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     5581 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     5641 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     5701 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5761 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5821 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5881 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5941 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6001 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6061 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6121 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6781 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8101 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     8161 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     8221 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     8281 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     8341 gcgttg
//
