LOCUS       sequence-441459-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula 
            gracilis D amino acid oxidase (DAAO) to measure transport of D amino
            acids across the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Johal AS, Al-Shekaili HH, Abedrabbo M, Kehinde AZ, Towriss M, Koe 
            JC, Hewton KG, Thomson SB, Ciernia AV, Leavitt B, Parker SJ
  TITLE     Restricting lysine normalizes toxic catabolites associated with 
            ALDH7A1 deficiency in cells and mice.
  JOURNAL   Cell Rep. 2024 Dec 24;43(12):115069. doi: 
            10.1016/j.celrep.2024.115069. Epub 2024 Dec 10.
  PUBMED    39661514
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-12-24"; volume: "43(12):115069. doi"; pages: " 
            10.1016/j.celrep.2024.115069. Epub 2024 Dec 10"
COMMENT     Sequence Label: pLentiCMV_HyPer7RgDAAO
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..83)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        68..397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      248..383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        445..492
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             511..909
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             996..1229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1301..1422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1338..1357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1392..1411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1462..1597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1617..1636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1618..1636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1646..1663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1646..1662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1655..1677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1804..2259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1891..1910)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2101..2122
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2285..2389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2390..2458,2459..3250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2608..2627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3421..4009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3910..3929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4163..4180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4297..4318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4333..4338,4339..4356,4357..4363)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4371..4387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4398
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4395..4411
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4395..4411
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4430..4450
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4432..4450
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4478..4704
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4705..4885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4932..5057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5550..5783
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5968..6012
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6279..6395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6418..6721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6722..6925
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6876..6896
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6922..6946
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7030..7054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     7671..7692
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7801..7822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8062..8081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             9604..9630
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(9635..9659)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(9690..10266,1..12)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9743..9763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10161..10172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgcctcccc gcctggaatt ctgcagatat ccggttagta atgagtttgg aattaattct
       61 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      301 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
      361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
      421 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
      481 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
      541 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      601 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      661 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      721 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      781 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      841 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
      901 gagggctaag cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
      961 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     1021 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     1261 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     1321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     1441 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1561 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1621 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1681 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1741 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1801 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1861 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1921 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1981 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2041 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2101 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2161 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2221 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2281 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2341 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2401 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2461 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2521 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2581 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2641 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2701 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2761 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2821 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2881 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2941 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3001 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3061 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3121 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3181 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3241 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3301 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4201 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4261 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4321 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4381 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4441 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     4501 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4561 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4621 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4681 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4861 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4921 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4981 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     5041 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     5101 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     5161 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     5221 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     5281 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     5341 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     5401 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     5461 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5521 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5581 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5641 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5701 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5761 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5821 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5881 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5941 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     6001 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     6061 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     6121 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     6181 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     6241 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     6301 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     6361 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     6421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     6481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     6541 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     6601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     6661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     6781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     6841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6901 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     6961 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     7021 cagatatcaa caagtttgta caaaaaagca ggcttcacca tgcacctggc taatgaggag
     7081 caaaacgaat tggaaggtgc ttttaagctg ggcttgatct ttaccgtcgc cccgtacttg
     7141 ctgcctaagt tgatcgtcag cctgcgccgc accgccccga agatgccttt gatgttggag
     7201 gagaattaca cccacaccct taccgagtca ctgaaacgcg gcgacgttga cgccatcatt
     7261 gtggcagagc cgtttcaaga gccgggtatt gtgactgaac ctttgtatga cgagccgttt
     7321 ttcgttatcg ttccgaaggg ccatagcttc gaggagttgg atgccgttag ccctcgcatg
     7381 ttgggcgaag aacaagttct gcttctgacc gagggcaact gtatgcgtga ccaggtgctt
     7441 agctccgccg gcttcaacag caacaacgtc tatatcatgg ccgacaagca gaagaacggc
     7501 atcaaggcca acttcaagat ccgccacaac gtcgaggacg gcagcgtgca gctcgccgac
     7561 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     7621 ctgagcttcc agtccgtcct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     7681 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caacgtggat
     7741 ggcggtagcg gtggcaccgg cagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7801 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7861 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     7921 gtgccctggc ccaccctcgt gaccaccctc agctacggcc tgaagtgctt cgcccgctac
     7981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     8041 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     8101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcggcttcaa ggaggacggc
     8161 aacatcctgg ggcacaagct ggagtacaac ggccccagtt gcagcgagct tgccgccaaa
     8221 cagcgcattc agggtctcac caacaccctt caaggctcaa gcatcaatac catccgccac
     8281 atggttgcca gtggcttggc aatcagcgtc ttgcctgcca ctgcccttac cgaaaacgac
     8341 cacatgctgt ttagcattat cccgtttgaa ggcaccccgc ctagccgccg cgttgtcctg
     8401 gcctatcgcc gcaactttgt ccgccctaag gccctttcag ccatgaaagc cgcaattatg
     8461 caaagccaac tgcatggtgt tagcttcatc tgcgatggtg gtagtggtca cagccagaag
     8521 agggtggtcg tgctgggctc cggagtcatc gggctctcta gcgccctgat tctcgctaga
     8581 aaaggctact ccgtgcatat cctggccaga gacttgcctg aggatgtcag ctcccagacc
     8641 ttcgcaagcc cctgggccgg agctaactgg acaccattta tgaccctgac agacggaccc
     8701 agacaagcca agtgggaaga gtccaccttc aagaaatggg tggagctggt tcccacaggc
     8761 cacgctatgt ggctgaaagg gaccagaagg tttgcccaga atgaggatgg cctcctggga
     8821 cattggtaca aggacatcac ccctaactat aggccacttc caagctctga atgtccacct
     8881 ggagccattg gagtgacata cgacaccctg agcgtccacg ctcccaagta ttgccaatac
     8941 cttgccagag agctgcagaa acttggggct accttcgaaa ggagaacagt gactagcctg
     9001 gagcaagcct tcgatggagc tgacctcgtg gtcaacgcca ccgggctggg agctaagtcc
     9061 attgccggca tcgatgacca ggctgccgag cccattaggg gacagaccgt gcttgtcaaa
     9121 tctccctgta agaggtgcac aatggatagc tccgaccctg cttctcccgc ttatatcatt
     9181 cctagaccag gaggagaagt gatctgtggc ggaacctacg gagtcggcga ttgggacctg
     9241 agcgtgaatc ccgaaacagt ccaaaggatt ctcaagcact gcctgagact tgaccctacc
     9301 attagctccg atggaactat cgagggcatt gaagtgctga gacataacgt tggcctgaga
     9361 cccgccagga gaggaggacc tagggtggag gctgaaagaa tcgtcttgcc cctggaccgg
     9421 accaagagcc ctctctctct gggaagaggg tccgcacggg ctgcaaaaga gaaggaagtg
     9481 acccttgtcc acgcctacgg ctttagtagc gcagggtatc agcaatcctg gggagccgct
     9541 gaggacgtgg ctcagctggt cgatgaagcc ttccaaagat accacggcgc agctagggag
     9601 agcctgcccc ccctggagcg gctgaccctg taaaacccag ctttcttgta caaagtggtt
     9661 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     9721 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9781 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     9841 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     9901 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     9961 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    10021 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    10081 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10141 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10201 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    10261 tttggg
//
