LOCUS       sequence-44149-e        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: Wnt10A STOP
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1965..2064)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2081..2100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2082..2100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2105..2121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2105..2121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2223..2242)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2234..3043
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2301..2320)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3190..3778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3679..3698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gggcagcgcc caccctcgcc cctggctgcg
      721 gctccgaccc cagccccagc cgcggccagc gctctgggtg ctcctgttct tcctactgct
      781 gctggctgct gccatgccca ggtcagcacc caatgacatt ctggacctcc gcctcccccc
      841 ggagcccgtg ctcaatgcca acacagtgtg cctaacattg ccaggcctga gccggcggca
      901 gatggaggtg tgtgtgcgtc accctgatgt ggctgcctca gccatacagg gcatccagat
      961 cgccatccac gaatgccaac accaattcag ggaccagcgc tggaactgct caagcctgga
     1021 gactcgcaac aagatcccct atgagagtcc catcttcagc agaggtttcc gagagagcgc
     1081 ttttgcctac gccatcgcag cagctggcgt ggtgcacgcc gtgtccaatg cgtgtgccct
     1141 gggcaaactg aaggcctgtg gctgtgatgc gtcccggcga ggggacgagg aggccttccg
     1201 taggaagctg caccgcttac aactggatgc actgcagcgt ggtaagggcc tgagccatgg
     1261 ggtcccggaa cacccagccc tgcccacagc cagcccaggc ctgcaggact cctgggagtg
     1321 gggcggctgc agccccgaca tgggcttcgg ggagcgcttt tctaaggact ttctggactc
     1381 ccgggagcct cacagagaca tccacgcgag aatgaggctt cacaacaacc gagttgggag
     1441 gcaggcagtg atggagaaca tgcggcggaa gtgcaagtgc cacggcacgt caggcagctg
     1501 ccagctcaag acgtgctggc aggtgacgcc cgagttccgc accgtggggg cgctgctgcg
     1561 cagccgcttc caccgcgcca cgctcatccg gccgcacaac cgcaacggcg gccagctgga
     1621 gccgggccca gcgggggcac cctcgccggc tccgggcgct cccgggccgc gccgacgggc
     1681 cagccccgcc gacctggtct acttcgaaaa gtctcccgac ttctgcgagc gcgagccgcg
     1741 cctggactcg gcgggcaccg tgggccgcct gtgcaacaag agcagcgccg gctcggatgg
     1801 ctgcggcagc atgtgctgcg gccgcggcca caacatcctg cgccagacgc gcagcgagcg
     1861 ctgccactgc cgcttccact ggtgctgttt cgtggtctgc gaagagtgcc gcatcaccga
     1921 gtgggtcagc gtctgcaagt gaattctgaa gggtgggcgc gccgacccag ctttcttgta
     1981 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2041 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2101 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2161 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2221 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2281 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2341 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2401 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2461 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2521 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2581 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2641 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2701 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2761 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2821 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2881 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2941 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3001 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3061 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3121 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3301 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     3601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
