LOCUS       sequence-44155-e        3762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: Wnt2B2 Non-STOP
FEATURES             Location/Qualifiers
     source          1..3762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1887..1986)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2003..2022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2004..2022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2027..2043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2027..2043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2145..2164)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2156..2965
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2223..2242)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3112..3700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3601..3620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gctgagaccg ggtggtgcgg aggaagctgc
      721 gcagctcccg cttcggcgcg ccagcgcccc ggtccctgtg ccgtcgcccg cggcccccga
      781 cggctcccgg gcttcggccc gcctaggtct tgcctgcctt ctgctcctgc tgctgctgac
      841 gctgccggcc cgcgtagaca cgtcctggtg gtacattggg gcactggggg cacgagtgat
      901 ctgtgacaat atccctggtt tggtgagccg gcagcggcag ctgtgccagc gttacccaga
      961 catcatgcgt tcagtgggcg agggtgcccg agaatggatc cgagagtgtc agcaccaatt
     1021 ccgccaccac cgctggaact gtaccaccct ggaccgggac cacaccgtct ttggccgtgt
     1081 catgctcaga agtagccgag aggcagcttt tgtatatgcc atctcatcag caggggtagt
     1141 ccacgctatt actcgcgcct gtagccaggg tgaactgagt gtgtgcagct gtgaccccta
     1201 cacccgtggc cgacaccatg accagcgtgg ggactttgac tggggtggct gcagtgacaa
     1261 catccactac ggtgtccgtt ttgccaaggc cttcgtggat gccaaggaga agaggcttaa
     1321 ggatgcccgg gccctcatga acttacataa taaccgctgt ggtcgcacgg ctgtgcggcg
     1381 gtttctgaag ctggagtgta agtgccatgg cgtgagtggt tcctgtactc tgcgcacctg
     1441 ctggcgtgca ctctcagatt tccgccgcac aggtgattac ctgcggcgac gctatgatgg
     1501 ggctgtgcag gtgatggcca cccaagatgg tgccaacttc accgcagccc gccaaggcta
     1561 tcgccgtgcc acccggactg atcttgtcta ctttgacaac tctccagatt actgtgtctt
     1621 ggacaaggct gcaggttccc taggcactgc aggccgtgtc tgcagcaaga catcaaaagg
     1681 aacagacggt tgtgaaatca tgtgctgtgg ccgagggtac gacacaactc gagtcacccg
     1741 tgttacccag tgtgagtgca aattccactg gtgctgtgct gtacggtgca aggaatgcag
     1801 aaatactgtg gacgtccata cttgcaaagc ccccaagaag gcagagtggc tggaccaaac
     1861 ctgcattctg aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag
     1921 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1981 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2041 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2101 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2161 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2221 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2281 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2341 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2401 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2461 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2521 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2581 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2641 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2701 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2761 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2821 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2881 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2941 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3001 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3061 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3241 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3481 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     3541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3721 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
