LOCUS       sequence-441588-        7927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY144
FEATURES             Location/Qualifiers
     source          1..7927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3286..3306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3328..3348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5518..5540)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5640..5659
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5737..5755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5823..5927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5928..5996,5997..6788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6146..6165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6959..7547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7448..7467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7701..7718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7835..7856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7871..7876,7877..7894,7895..7901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7909..7925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7914..7927,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tttatcacga
      181 gcttctggat ctttaaaact gaaaaaagcc tgagcgacaa aaatagaaac acaaagaaat
      241 tcagtttttc cagatttggg ggtcttcatt ttcctgaaaa ttttagaaca cagctgccaa
      301 taacacatac ttggtatttt tgtagataat ggtcccttta caatatgttc aattggtaat
      361 ggtttcccat atgtgaataa aaagtaattt aataaactta aacctcctaa aattttcaaa
      421 atctcaagtt gaaattgtaa attttcagtt ttcaaaaaaa aattaacgtt atcaagaaat
      481 tttgaacgct taaaactcga atcaattgag aaacatttga acaaataaat ttcaaatcgt
      541 gtttaaaaaa atgtatccaa tttcagaagg attacaataa tttcagattt aaatagtaga
      601 tcatatactt ttaacacctg cgattgttat ctgatctaca gtttcgataa gaaaggtgtt
      661 gactgctgct ccaactcccc aaattggtga cgagagcaga tggatccgca ggaagtacaa
      721 attgttctcc atctgcaacc agacattatg tgccacgtgg tagtcgattg aggtgctata
      781 aatgaccaga tattgaccgt ttttggaatc taattacaga caatgaacgt attcaactaa
      841 cagctgtata gagtatgtgg gtgtctggtg acttatgact tatatggtta tgacactaaa
      901 tttgaagagt gtcgaatatt gcagcagatt tgcaaattcc ccataattcg aggtattgtt
      961 tgcaacaata gatattattg ttccttataa ttcatcaaac aatattaatc agcattttgc
     1021 atttgtttga aagtaccaaa ttttgtaaaa gccttgaata gggatggcaa accgagcgcc
     1081 ctgtttttca aaagtaattt ctcgagtttt aatttggttt taaaaaaacg atgttcagat
     1141 attggaacaa gccgaaacta tcgaatcagc taaaaaacag gcaacgaata cgacaagtat
     1201 ccgtaaaacc aaactccgta gttattccga tgcgccggct atagttctgc tcacctctga
     1261 agcaaattca ttgggcagat tgatggtata actaaatttt aaaaaagcta aataaattaa
     1321 aatgttcaaa aatgattcgt cagttgacat tttttgataa tagatacatc agaaaacata
     1381 ttatacaagt taatactgca tgaattgaaa tgtaaaaaaa gagtttacaa tgggaatgta
     1441 gcataagtac cacccacagt caactgcgaa tttacgacaa aaaataaagt tacaaaagtt
     1501 tagaaaaaac aatgtaatta tcagtttatc tgatatcccg tatttttata tcttggttca
     1561 ctttggtttt atcaaaaatt tgtcaactga caaaatactt gtcacaattt tttctacatt
     1621 ttaccagtaa aatttgtttt aactaaaacc aaagaaaacc gaaatataaa ctgtcaaaat
     1681 aaaacaatgg ggatgtaatt ttattaggta aaatatggtg acaataaata gccaatatca
     1741 aatctacaaa agaggaatag ttgatgtaca ctactaatga aagcaaatgg tataaagaat
     1801 acgcctcttt gcattattga ccgactaccg tatgctcgtc aatcaatgtt ttgctaggag
     1861 aaaagagcac gtcagttcaa ttgttgagga ctgtagaaat tggtgacaga tgtgatgaaa
     1921 aacgagaaca tgattatcaa ttggattacg tgggtacttt tttaattgaa gtcactaact
     1981 ataaataaaa cacttggaat ttcttttagc cggctttctc gaagttcata tgttgataaa
     2041 tataaaaata gagtttcaat atttctattt gctctaaatt cttcaaaact accgaaaaat
     2101 taattattac ccaattaaat actataaaaa attaatttct taatgagacc tcttgaaacc
     2161 ttcctgcaat tttttttgac ggttcaaaac ttttcaattt tcccagttgg tccgtgtcgc
     2221 agcggttaaa ttaagtgggt gcaagaaagg taataaaatt tgtgcacact tagccattat
     2281 aaaaaaacaa caaaaactca aaataaataa ccaaaataag caagatatag aattaattgt
     2341 ataaaagata atttccggtc actgtcaaca agctgtgaaa attagaaatt atagctaaaa
     2401 cctattttat tgcagtccac ttgaacttta aaaatgatta aaactttgtg aaattttgaa
     2461 gaattcgtat aaaaattcgg tcttatgaga tcagtttaag ttttgtagtg aaaaaaatac
     2521 atttcagttc tccaagtaac actgttttat ttcttctaac tatacgagca agtttcaagt
     2581 tcatttacgc ccacaaaaat ttcaaaacta ttctcccaaa aatgtgtatc ttattattca
     2641 ctacagtaat ctgagcccat gtttctttca aaatgttcat cccctcattc cacgacaaaa
     2701 cgagaatgtt tagctattaa ctggctgctt aaaaattcaa aagtctcaat gaaaggaaag
     2761 gtaatctgag actgcacttt gtttccatat ttcttcttct tcttttcagc tcttcttatt
     2821 ttttcaaact tcagcctctt cgtcatttcg ttccggtctc agccatactg tcaggagtca
     2881 gtccggaaat catcatccaa ttaatatctg cagaggccca tcctcacctc ctaacaacgt
     2941 gagggctcct atttcaaacg agaagctact cgaattcgac acttttattc tcgcctataa
     3001 ttaatcctca acacaaattt ccctcccacc acctcatccc aaaagagctc tacaggtgcc
     3061 tccccctctt ggactgttgt accgtacccc tcaacacaat attgctcgat tagccccgcc
     3121 catttggtct tctaggaacg tttgcaagat gagtagtatt ccaccttatt tcccgggatt
     3181 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     3241 aggacccttg cttggagggt accgagctca gaaaaaatga ccgctccaaa gaagaagcgc
     3301 aaggttccag tcgagaagat gactgcccca aaaaagaaac gtaaggtccc gcgggtaagt
     3361 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagcgcgc tgtgtcaaaa
     3421 ggagaagagc ttattaagga gaatatgcgt tccaagctct acctcgaggg atccgtcaac
     3481 ggacaccaat tcaagtgcac ccacgaggga gagggaaagc catacgaggg aaagcaaacc
     3541 aaccgtatca aggtcgtcga gggaggacca ctcccattcg ccttcgacat cctcgccacc
     3601 cacttcatgt acggatccaa ggtaagttta aacatatata tactaactaa ccctgattat
     3661 ttaaattttc aggtcttcat caagtaccca gccgacctcc cagactactt caagcaatcc
     3721 ttcccagagg gattcacctg ggagcgtgtc atggtcttcg aggacggagg agtcctcacc
     3781 gccacccaag acacctccct ccaagacgga gagctcatct acaacgtcaa ggtccgtgga
     3841 gtcaacttcc cagccaacgg accagtcatg caaaagaaga ccctcggatg ggagccatcc
     3901 accgagacca tgtacccagc cgacggagga ctcgagggac gttgcgacaa ggtaagttta
     3961 aacatgattt tactaactaa ctaatctgat ttaaattttc aggccctcaa gctcgtcgga
     4021 ggaggacacc tccacgtcaa cttcaagacc acctacaagt ccaagaagcc agtcaagatg
     4081 ccaggagtcc actacgtcga ccgtcgtctc gagcgtatca aggaggccga caacgagacc
     4141 tacgtcgagc aatacgagca cgccgtcgcc cgttactcca acctcggagg aggaatggac
     4201 gagctctaca agcctaggat accggtgggt gaagacccac caaagccatc tgccaaggga
     4261 gccaagaagg ccgccaagac cgttacgaag ccaaaggacg gaaagaagag acgtcatgcc
     4321 cgtaaggaat catactccgt ctacatctac cgtgtcctca agcaagttca tccagacact
     4381 ggagtttcct ccaaagccat gtctatcatg aactcttttg tcaacgatgt cttcgagcgt
     4441 attgctgctg aagcatcccg tcttgctcac tacaacaagc gttccacaat ctcatcccgc
     4501 gaaattcaga ccgctgtccg tctgatcctt ccaggagagc ttgccaagca cgccgtgtct
     4561 gagggaacca aggccgttac caagtacact tccagcaagt aggaattcca actgagcgcc
     4621 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     4681 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     4741 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     4801 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     4861 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     4921 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     4981 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     5041 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     5101 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     5161 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     5221 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     5281 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     5341 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     5401 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     5461 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     5521 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     5581 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     5641 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5701 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     5761 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5821 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5881 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5941 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6001 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6061 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6121 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6181 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6241 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6301 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6361 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6421 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6481 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6541 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6601 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6661 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6721 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6781 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6841 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6901 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6961 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7021 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7081 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7141 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7201 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7261 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7321 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7381 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7441 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7501 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7561 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7621 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7681 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7861 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7921 aacaatt
//
