LOCUS       sequence-441605-         143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX459 plasmid encoding the sgRNA protospacer sequence 
            "5'-GCATAATGTCACTACAGATC-3' to target the LIG4 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 143)
  AUTHORS   Cortazar MA, Jagannathan S
  TITLE     SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through 
            Selectable Homology-Directed DNA Repair.
  JOURNAL   Methods Mol Biol. 2025;2863:397-417. doi: 
            10.1007/978-1-0716-4176-7_23.
  PUBMED    39535722
REFERENCE   2  (bases 1 to 143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2863:397-417. doi"; pages: " 
            10.1007/978-1-0716-4176-7_23"
COMMENT     Sequence Label: pX459_LIG4_Exon3
FEATURES             Location/Qualifiers
     source          1..143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgca taatgtcact
       61 acagatcgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgc
//
