LOCUS       sequence-441614-        6668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srx-105 fluorescent neural reporter driving nuclear CyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY131
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2027..2047
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2069..2089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4259..4281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4381..4400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4478..4496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4564..4668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4669..4737,4738..5529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4887..4906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5700..6288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6189..6208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6442..6459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6576..6597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6612..6617,6618..6635,6636..6642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6650..6666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6655..6668,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga catcctttgg
      181 tggaatcctt atgaacttgt acatgttcta tcatttttcc aaattgcaaa aaactagttt
      241 ctacattctc tgcgcttcga aaactgtctc aaatacctta atgcttctta cctacctttt
      301 atacgttggt ccagttaatg ttctgtaaga gttttgaaat ccactgaaac tttaaaatga
      361 tactgatgtt ttaaaggtac acagcaatag gctacagtga gctgaatagc tacgtaaatc
      421 agatgaattc cttcggtttt tatttgcaag gtaggtagct cagtttggac attttccgcg
      481 actctgctct tttaggacct ataactcagc ttatgataac tgtgaatcga ttcttggtcg
      541 tttggatttc cgccgcaaaa actacaaata gtagtacaaa agttacagta atagctctag
      601 caatttcctg gatttttgcc gcgtggctct ctactctggc gggacttcca ggtatgctgt
      661 cccgtaaact gtcggctgcc agcaaaatca atttttcaga tgactgtcgt atgcatgtga
      721 actttggtca tgtaccatgg actcggcctc cgtgtgctta agagatgatc aatttacttt
      781 ttatcgctgt attttcactg ggggtttcta ctaatgttat gaacgttttt atagcaatca
      841 agttgttcat tttatcggta agttagagta ggtgcagccg acaagttatg ggttcctaca
      901 atgatcgcgt aaatgtgggc agccgacata ttacgggttc atagaaatat cgtcttgggg
      961 ttgacagtat tttgcctact tgcaattgcc aattgccttc tctaaaaacg tggatcagct
     1021 acagttactt ccgagtcagg aatgcccaaa tagccttttt gatacagaaa aacaccgaca
     1081 agttaggggt tttctaatga gtcaaaaaag gaagcagacg acaaattatt ggtttgtctt
     1141 tgagttcgtc tgccgcccgc gcaattggat gacgcagccc acaaattaca taacgttttc
     1201 tacgagttag cagcggactc aactttgtca acatttcaga aatcctcaca actactctcc
     1261 tcaaacacat ctcaaagccg gaagaaaaac cgaatctacc tatttctaca gtcttgtttt
     1321 caagattgga tttgcgtcct ggacattgtt aacaacatga tatccggctt ttactgtacc
     1381 gacagaacat gtatagttct ggtgacaatg ggattcgggg tgacggttta tttggcggat
     1441 gggtagggtt tctgaaaatg tacaataaaa atatagaaaa ttatttcagc gttctcatgt
     1501 atctgttcaa ctgcaagtta aactctgatt ccaagatgag cccaaacaag ttgaagaatg
     1561 cgaaaacttc tcagaatgct aaaaagtcca cggtcacccc gatttcttga atattcccct
     1621 aataagtccg ttataatttt ccctgttcgt tacatttttt ctgcaaaata gagctcatat
     1681 atttgcctga atttcgagat ttttcagaca aaagctcacc gataacatat catttttagc
     1741 actcaataat ttgttagtca tttttgatat gttcttcgaa attctgactt gaatgagctt
     1801 ttccaagcaa gtcaccacat tttgattggt tttgtggtaa ttgagttttt tttttttgat
     1861 tatttctgtt ctctctgctg gctcgagcaa caagttcaga gatattttgg cacccgggat
     1921 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     1981 gaggaccctt gcttggaggg taccgagctc agaaaaaatg accgctccaa agaagaagcg
     2041 caaggttcca gtcgagaaga tgactgcccc aaaaaagaaa cgtaaggtcc cgcgggtaag
     2101 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtgtcaaa
     2161 aggagaagag cttattaagg agaatatgcg ttccaagctc tacctcgagg gatccgtcaa
     2221 cggacaccaa ttcaagtgca cccacgaggg agagggaaag ccatacgagg gaaagcaaac
     2281 caaccgtatc aaggtcgtcg agggaggacc actcccattc gccttcgaca tcctcgccac
     2341 ccacttcatg tacggatcca aggtaagttt aaacatatat atactaacta accctgatta
     2401 tttaaatttt caggtcttca tcaagtaccc agccgacctc ccagactact tcaagcaatc
     2461 cttcccagag ggattcacct gggagcgtgt catggtcttc gaggacggag gagtcctcac
     2521 cgccacccaa gacacctccc tccaagacgg agagctcatc tacaacgtca aggtccgtgg
     2581 agtcaacttc ccagccaacg gaccagtcat gcaaaagaag accctcggat gggagccatc
     2641 caccgagacc atgtacccag ccgacggagg actcgaggga cgttgcgaca aggtaagttt
     2701 aaacatgatt ttactaacta actaatctga tttaaatttt caggccctca agctcgtcgg
     2761 aggaggacac ctccacgtca acttcaagac cacctacaag tccaagaagc cagtcaagat
     2821 gccaggagtc cactacgtcg accgtcgtct cgagcgtatc aaggaggccg acaacgagac
     2881 ctacgtcgag caatacgagc acgccgtcgc ccgttactcc aacctcggag gaggaatgga
     2941 cgagctctac aagcctagga taccggtggg tgaagaccca ccaaagccat ctgccaaggg
     3001 agccaagaag gccgccaaga ccgttacgaa gccaaaggac ggaaagaaga gacgtcatgc
     3061 ccgtaaggaa tcatactccg tctacatcta ccgtgtcctc aagcaagttc atccagacac
     3121 tggagtttcc tccaaagcca tgtctatcat gaactctttt gtcaacgatg tcttcgagcg
     3181 tattgctgct gaagcatccc gtcttgctca ctacaacaag cgttccacaa tctcatcccg
     3241 cgaaattcag accgctgtcc gtctgatcct tccaggagag cttgccaagc acgccgtgtc
     3301 tgagggaacc aaggccgtta ccaagtacac ttccagcaag taggaattcc aactgagcgc
     3361 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga
     3421 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg
     3481 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     3541 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     3601 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     3661 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     3721 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     3781 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     3841 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     3901 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     3961 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     4021 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     4081 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     4141 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     4201 ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4261 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4321 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4381 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     4441 gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta
     4501 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4561 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4681 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4741 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4801 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4861 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4921 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4981 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5041 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5101 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5161 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5221 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5281 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5341 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5401 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5461 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5521 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5581 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6361 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6421 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6481 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6541 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6601 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6661 taacaatt
//
