LOCUS       sequence-441616-        7929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-17(3k) fluorescent neural reporter driving nuclear mCyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY142
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3288..3308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3330..3350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5520..5542)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5642..5661
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5739..5757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5825..5929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5930..5998,5999..6790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6148..6167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6961..7549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7450..7469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7703..7720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7837..7858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7873..7878,7879..7896,7897..7903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7911..7927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7916..7929,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga acgaagctct
      181 agccatggaa gtttgaattg aaaacctaaa ccccgatgtg tattcatttg agcttaagcc
      241 taagcctaat tctacgccta agcataagcc taagcctatg ttcaagccta agccaaattg
      301 tagaacgtta ccaaaagatc aaacctattt ttcctacaga aaaggtctaa tgataactgt
      361 taagcgagag acaaggagag atatatatca aaaactagcc caaactgtga ttaacatcta
      421 ttaactgacc tcaaaaaaaa aaatagtaga gattaaatac tgctcaaaaa gtttcttgga
      481 aaagtaaatt gttaggtttc tggggcctgt aaaactggac cgagttttga agaatttttt
      541 taacttttca aagtttggcg tcactgaaag ctttatattg tacatgttcc accaattttc
      601 ttttgtatcc gttttttctg tctcaaaacg gagctcgagc ccatgacgtg tggataccgt
      661 gagaatccgc ccaattcctt cacatcgatc tcatatttct ttcccagttt atattgaatt
      721 cccgttgaat tgcggctttg caggggagaa gatgtgcgtc tttatatttt tttttctatc
      781 cttacccgcc gtgaatccgg ctcgaattga gccaattttt atgaatagta gagctaattg
      841 acttcgtctc cctagaattg atgaggtaaa gctcgtgggg agatatggag atttttcgat
      901 tttttttcca gtaattttca gttcaaaatt tgagcttttg agcaggagtt ttgagatttg
      961 tgccaaaatt tcctattatt ttttcaaata tttttgcttt tgtaaaaaag gtgtagtaga
     1021 agttttgtag tgccactatt ttttttttga aaattatgtt ttggcggaaa gttctttaaa
     1081 aaagttaatc aaaatttttg gcgctttagt caaattaatt ttctggcaaa aatattagta
     1141 attttggcgt gagttcaatt ttcccattct taaaatttca cacaattaag aattttaaga
     1201 tttgggtttt tttttcaaca aaaaatcaaa tatttacatg aaaattcggc gggaaataaa
     1261 aaaaaatatt ccgattcaat gtgtgaataa ctttttgcgg ggaattcaaa tttgcattat
     1321 ttaaactttt tttaagctcg aaaaattgaa attcttaaat aaataaataa aattttcctc
     1381 gaattttgaa tgtttcgatt ttttagcgtt attttttcaa aatttccact aaaactctag
     1441 ttctaaagtt ctaaaacttt aaaaacttcc taatgttgtg gtcttgtgtt tttttggcag
     1501 cgatttccaa atttctgaaa ttccaacaaa aaaaccacta cgtcagtttg catatccaac
     1561 cccccccccc cccccccctc tgagccaatc aatcatgtgc attttgaatt ggcgccaagg
     1621 caatcttcgt aaaaaataac aatagagtca tccctcagaa cagaaaagaa acccgcccgg
     1681 gggctcaaat acacatgtca tttttctttt ttcgcctgat ttttagccta aattagtgtt
     1741 tagcaatttt ttaaggtaac tcggtggagt catttgaact ttttaaacta aaatttcaga
     1801 aaaaattctt tttttttgtc aaaaaataat tttcccgccg gaatttttca aaaaaataat
     1861 ttttatttta tttttcaagt tccttgtttc ctctttattt ttttattttg cccccaaaag
     1921 ttttacacta aaatatttaa ttttcaggta attcatcgaa agtctttcta ttttccgcat
     1981 ctcttgttca aaatagtaac ctactaaaac aaggtgagcc taattttttt taaatactgt
     2041 atttccaaaa agtagtcata agatatatta tgaaaatttt caagaatctt cgtacaatat
     2101 tttggcagaa aatttgaatt ttgaaatttt ttggtaaatt ttcctggaat atttgaaatt
     2161 aatttttggc gccagtttca aaacataaaa tttagaattt tttttaagtt ttgaaaaaaa
     2221 tttttcaaca aattttggcg gtaatttgtg aatttctttt tttttaaaca tatttcgaaa
     2281 aaattatttt gacataattt tttcaataaa aaaatttgca caaagtttaa aataagttgt
     2341 tcaaaaaatt ttctagaatc tcgatttaaa aaatttctga cgaatatgcc gcaaacttac
     2401 atattagaaa aaaaattggc gggaaataaa agaacacaga atttttcata aaattttaaa
     2461 taatttggcg caaagtctaa aaattgttct gaaaaagatg ttttttcgac aaaattcgaa
     2521 tttatcaatt ttctaaaaaa atatttccaa agaaagagtt tagcgcgaat ttgaaatttt
     2581 tgaaaaagtg acagttgtca tactcaatta tttgcaaaaa atttttaaat aagacaattg
     2641 gcgcgaagtt caaaaattcc agaaaaaatt ttgcgcaaaa gtcaaaattt tgagagtttt
     2701 tggcgcgaaa ttcatatttt tcaaaaaaaa attggcgggc tatacaaaaa ttcaaaaaaa
     2761 ttcggcaaaa aatacaaaat ttttaacatg ttcaacaact aaaccaccaa gaagttcctc
     2821 tttaaaaaaa tttccaaaaa ttacaaactc ctcctgtatt acctgtattt ccactacctt
     2881 tcttatatta attttctcaa ttttctcctg tatttctctg tctctcccct ccccaacaag
     2941 ccttatacgt gtgtttttcc tcaaaactgg gcgtggtctc ggcggccctc tggcgatctg
     3001 tgtctcccct aagtctctct ctcccccata caatgggtct cacggctctg ttacgcgccg
     3061 agccacgtca taagttttct ttaaattttt ttttgtttga gaaaaagccc ctaggccagc
     3121 ccgaagccca aaaaatttag aaaaaataaa atattccagg gggagagaga gagcccggga
     3181 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     3241 ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gaccgctcca aagaagaagc
     3301 gcaaggttcc agtcgagaag atgactgccc caaaaaagaa acgtaaggtc ccgcgggtaa
     3361 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagcgc gctgtgtcaa
     3421 aaggagaaga gcttattaag gagaatatgc gttccaagct ctacctcgag ggatccgtca
     3481 acggacacca attcaagtgc acccacgagg gagagggaaa gccatacgag ggaaagcaaa
     3541 ccaaccgtat caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca
     3601 cccacttcat gtacggatcc aaggtaagtt taaacatata tatactaact aaccctgatt
     3661 atttaaattt tcaggtcttc atcaagtacc cagccgacct cccagactac ttcaagcaat
     3721 ccttcccaga gggattcacc tgggagcgtg tcatggtctt cgaggacgga ggagtcctca
     3781 ccgccaccca agacacctcc ctccaagacg gagagctcat ctacaacgtc aaggtccgtg
     3841 gagtcaactt cccagccaac ggaccagtca tgcaaaagaa gaccctcgga tgggagccat
     3901 ccaccgagac catgtaccca gccgacggag gactcgaggg acgttgcgac aaggtaagtt
     3961 taaacatgat tttactaact aactaatctg atttaaattt tcaggccctc aagctcgtcg
     4021 gaggaggaca cctccacgtc aacttcaaga ccacctacaa gtccaagaag ccagtcaaga
     4081 tgccaggagt ccactacgtc gaccgtcgtc tcgagcgtat caaggaggcc gacaacgaga
     4141 cctacgtcga gcaatacgag cacgccgtcg cccgttactc caacctcgga ggaggaatgg
     4201 acgagctcta caagcctagg ataccggtgg gtgaagaccc accaaagcca tctgccaagg
     4261 gagccaagaa ggccgccaag accgttacga agccaaagga cggaaagaag agacgtcatg
     4321 cccgtaagga atcatactcc gtctacatct accgtgtcct caagcaagtt catccagaca
     4381 ctggagtttc ctccaaagcc atgtctatca tgaactcttt tgtcaacgat gtcttcgagc
     4441 gtattgctgc tgaagcatcc cgtcttgctc actacaacaa gcgttccaca atctcatccc
     4501 gcgaaattca gaccgctgtc cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt
     4561 ctgagggaac caaggccgtt accaagtaca cttccagcaa gtaggaattc caactgagcg
     4621 ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag
     4681 agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc
     4741 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     4801 ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt
     4861 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     4921 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     4981 aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt
     5041 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     5101 gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc
     5161 gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa
     5221 ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg
     5281 tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt
     5341 ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt
     5401 ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt
     5461 cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5521 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5581 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5641 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5701 tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt
     5761 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6061 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6121 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6181 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6241 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6301 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6361 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6421 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6481 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6541 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6601 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6661 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6721 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6781 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6841 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7621 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7681 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7921 ataacaatt
//
