LOCUS       sequence-441617-        6100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srx-113 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY130
FEATURES             Location/Qualifiers
     source          1..6100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1775..1795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1820..1840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1862..1882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3691..3713)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3813..3832
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3910..3928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3996..4100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4101..4169,4170..4961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4319..4338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5132..5720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5621..5640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6008..6029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6044..6049,6050..6067,6068..6074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6082..6098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6087..6100,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aggggtccag
      181 ctgtggcaag tactacagtt gttccaatca caacaacttc ttcgtcttcc acgacatctt
      241 ctaccactac tacaactgtc ccagtatcta caaccacatc tacatccagt actactacga
      301 ctactgtacc atctaccaca acctccagtg ctactacgac tactgtacca tctaccacaa
      361 cctccagtac aactacaact accgtaccac caacaacaac atctactacc actacaacta
      421 ccgtaccacc tacaacaacc tccactacta ctacaactac cgtaccacca acaacaacat
      481 ctactaccac tacaactacc gtaccaccta ccacaacctc cagtaccact acaactaccg
      541 taccaccaac aacaacatct agtaccacta caactaccgt accaccaaca acaaccacca
      601 gtacaactac aactaccgta ccaccaacaa caacctccac tactactaca actaccgtac
      661 cagcaacaac aacctctact gccactacaa ctaccgtacc accaacaaca tctactacca
      721 ctacaactac cgtaccacct acaacaacct ccactaccac tacaactacc gtaccaccaa
      781 caacaacatc tagtaccaca acgacaacga ctactacgct accaactacc acaacaacaa
      841 tgatgattcg taagttcttg aataaaaaac agaaataaat attggaatac gaatttcagc
      901 gatttgttct ttggattcac ttactttcgg aaaaggagac aactttcaac ctcaagtgga
      961 tatcgacgta acttattcaa agtatatatt tttcatattt gaacaaacgc attttctttt
     1021 cattcagctt ggttgctaac ataattcctg gcacaatgga tatgcaatca ctttctacaa
     1081 tgcagatcag ttgcactgcg atagccggat acaatgcgta tatgatcttc gatgtcttca
     1141 atcagccggc tgaaaatcaa ggatttccac aatcagtgac agttaatgca cagtgcaaca
     1201 gtgcagacat gatttggtat tatgtattag tgttgaatgg ggtcacatat acaagagtta
     1261 ttaattccat tacatgccaa caagcaccaa attgattgtg tcagaaacat tttacgtcaa
     1321 ttgtgtcaga aacattttac gttgtattcc tgatctgtga aaatgataat aataataata
     1381 atacttaata aatttgattg aattttaaaa atttcaatag cttgaagata aatgtatttt
     1441 cattccctcc tttttcaaag ttggtcaagt agaaaaatat ttcacttgcg acatcaggct
     1501 gaaattgcat aacaaacaac aaacatttag actcggaaaa ttaagggtta cctgcgtgca
     1561 aaaagtaata cattttcaaa ttggcaccta aaacttctat aaatactggc tcgggcctaa
     1621 gaaacaacga aacaaacccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     1681 taacataaca tagaacattt tcaggaggac ccttggaggg taccggtttt cccagtcacg
     1741 acgcgtggta ccgagctcag aaaaaatgac cgctccaaag aagaaacgca aagtaccgag
     1801 ctcagaaaaa atgacggcac ctaaaaagaa acgcaaagta ccggtcgccg agaagacagc
     1861 cccaaaaaag aagcgtaaag ttccacgcgt catgggagta agtttaaaca gttcggtact
     1921 aactaaccat acatatttaa attttcagcg cgctgtctcc aagggagagg agctcatcaa
     1981 ggagaacatg cacaccaagc tctacatgga gggaaccgtc aacaaccacc acttcaagtg
     2041 cacccacgaa ggggagggca agccctacga gggcacccag accaacagga ttaaggtggt
     2101 ggagggaggc cccctgccgt tcgcattcga catcctggcc acctgcttta tgtacgggag
     2161 caagaccttc atcaaccaca cccaaggaat cccagacttc ttcaagcaat ccttcgtaag
     2221 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagcctg agggcttcac
     2281 atgggagaga gtcaccacat acgaagacgg gggcgtgctt accgttaccc aggacaccag
     2341 cctccaggac ggctgcttga tctacaacgt caagctcaga ggggtgaact tcccatccaa
     2401 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc agtaccgaga ccctgtaccc
     2461 cgctgacggc ggcctggaag gcagatgcga catggccctg aagctcgtgg gcgggggcca
     2521 cctgcactgc aacctgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     2581 gccaggagta agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt
     2641 ctactttgtg gaccgcagac tggaaagaat caaggaggcc gacaatgaga cctacgtcga
     2701 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact
     2761 taatggcatg gacgagctgt acaagtaagc gccggtcgct accattacca acttgtctgg
     2821 tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta
     2881 acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt
     2941 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     3001 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     3061 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     3121 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     3181 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     3241 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     3301 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     3361 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     3421 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     3481 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     3541 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     3601 tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg
     3661 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3721 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3781 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3841 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     3901 gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4321 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4621 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5881 cagtgagcga tgaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6001 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6061 cggctcgtat gttgtgtgga attgtgagcg gataacaatt
//
