LOCUS       sequence-441628-        7352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tag-168 fluorescent neural reporter driving nuclear TagRFP-T 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY136
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2956..2976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3001..3021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3043..3063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4943..4965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5065..5084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5162..5180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5248..5352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5353..5421,5422..6213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5571..5590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6384..6972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6873..6892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7126..7143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7260..7281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7296..7301,7302..7319,7320..7326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7334..7350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7339..7352,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcgatccat aggtatgcct
      181 gtcattctct atttttaaac gttcctgcag cagtagcaca acagagccag cagttttcct
      241 cttcttctcc atcttcttct gcttcttcct ctcttcccaa aaaaaaaaaa aaaaaaaaaa
      301 aaagtgaggg ggctcactgc ccccccccct cccccccccc tcttctcctt gaagctcatc
      361 cagacgtcgc tgtcctcctc ctcctcgtcc ccggggcgct gtgtgtgtgt gtgtgtgctc
      421 cgtgagcatg tagcagcttc agcccattta aaggcgcaca ctctctgcat ctctgcgtgt
      481 ctcgacactc gttttttctc ttcttttctg ctgcacacac acacacacac acacacacgc
      541 tcatgccaga gcacttcttg ctccgggggg ctccgcgcgg agcccgagag gacctcctcc
      601 tcctttttct tcttcgcttt ttagttcttc ttgcctcggt ctcgcccctt gttttgctct
      661 ccatgtagta tatacatctg ggtgtgtgtg tgtgtgtgca cctttggagc attttttgaa
      721 cctttccaga actttggagc aacattttca gcttgttcag tgcacttttt aggctgaaaa
      781 tcctattttt aggttttcga aagttttttt ggagcatttt tcgtgcaatt taggtgcaaa
      841 attaaaaaaa agggttttgt agtgaattaa aatttttttg aaactttcgc ctttttaaaa
      901 aaatgtttct aatatcttta aaaattggaa tattttggaa aacaatttgt tttcaattgg
      961 caatttatta attctgagct tctgagcgta aatttttttt tattttggag caattctgag
     1021 atgtcttagt gcatttttcg ggcatacata aaaaaaaatt tatcttaata ataaaaacct
     1081 taaatttagg cttcaccgtc tggaaattca attttttcag ctgaaaaact ttcaaaaaaa
     1141 aaaaaattaa attttcacat ttattttctc tggaattttg gagcaatttc aaagctgtct
     1201 tagtgcattt ttagtacaca aatatttttt catttttctt aaattttaat aatctaagct
     1261 taaataaagg cttcaccgtc tgaaaatgca acttttttca gatgaaatat taaaaaaaaa
     1321 acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca tgtaatatgt
     1381 acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta caatttgaaa
     1441 atttttatta aaatttcaaa aaaatttttt ttttttttaa tttcgaattt ttttaaactt
     1501 gttgtaactt ttttccctgt cggcgccaat taacaaaata cgtagaattt tgcgcttatt
     1561 gttttgttag ttaacaaatt tttgacaact ttcttatcag ctgagattta ggtgtttcag
     1621 ttcgcaatct gcgtctctcc ttgctctcct ctattttaaa acccccataa ctttgttctt
     1681 gtgagagtta tcaaaaaatt gtcaactaac aaaatataga gcacaaaaat atgcacattt
     1741 tgtatgttga caattttttg ataccttttt tagttttcga gatatcttag ttttagttta
     1801 gctgtacctt tttctggcaa aaaacttcca aatttcggca atttttcggc aaatttcccg
     1861 gcaaatttcg ccaagtacat cgggctctca gacaagtggt ctgtgagtgg aagtcgataa
     1921 aagtagttta cgtgctctcc gtccccccgc ttcttctccg tctctgcttc tatttctcca
     1981 ttgactagtc ccactacttt ttacggggcg gagggctctc ttgcactcca gcttcttctc
     2041 tttttgtgct cttttttgcc cgtatgtggg tgctcattta tttttctttg gttttaaaat
     2101 aaattcggtt tattaggcta ctcgtaaaaa tgaagaaaaa acttcaaatt caaacgacat
     2161 taaaaaaaaa tttaataaaa attttttgaa ttgctcaaaa gccccaccta tatatatata
     2221 tagctccgcc tacttctcgc acagaccccg cccacttttt gactccgccc ccttattggc
     2281 aaaagttctt gaaaaaaata aaaataaaaa ttcaaattga aacaattttt ttttaatgaa
     2341 gtagtttttt tctaaattga tgaaaatttc taaacagttt acaagcaccg cccacttttt
     2401 gactccgccc ctttgttgga aaaattcttg taaaaaaaaa taaaaaataa aagcttctct
     2461 ttcaaacgat ttttagttgg tctttgtatg aaaagttttt ttcctaaaat agaaatgtct
     2521 gaataatttg caagctccgc ccttttctta cccagactcc gcccctttat tgactaattt
     2581 ttttttgtcc atttacctga agttcaaaaa aaaaatttac agtttcatag agtaataatt
     2641 tttcaaaaat ctctttttcc aatgtttaat gtgtcattat aaacccgccc agttttaagc
     2701 tccgcccatt taggccccgc ccatttatga cccaaatatt tttttctcta ttcacccgtg
     2761 gatttccctc caacaagtta ccccaaaaat aaaaataaaa attcctgcag gcgcccaccg
     2821 ggatctggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt
     2881 ttcaggagga cccttggagg gtaccggttt tcccagtcac gacgcgtggt accgagctca
     2941 gaaaaaatga ccgctccaaa gaagaaacgc aaagtaccga gctcagaaaa aatgacggca
     3001 cctaaaaaga aacgcaaagt accggtcgcc gagaagacag ccccaaaaaa gaagcgtaaa
     3061 gttccacgcg tcatgggagt aagtttaaac agttcggtac taactaacca tacatattta
     3121 aattttcagc gcgcttccaa gggagaggag ctcatcaagg agaacatgca catgaagctc
     3181 tacatggagg gaaccgtcaa caaccaccac ttcaagtgca cctccgaggg agagggaaag
     3241 ccatacgagg gaacccaaac catgcgtatc aaggtaagtt taaacatata tatactaact
     3301 aaccctgatt atttaaattt tcaggtcgtc gagggaggac cactcccatt cgccttcgac
     3361 atcctcgcca cctccttcat gtacggatcc cgtaccttca tcaaccacac ccaaggaatc
     3421 ccagacttct tcaagcaatc cttcccagag ggattcacct gggagcgtgt caccacctac
     3481 gaggacggag gagtcctcac cgccacccaa gacacctccc tccaagacgg atgcctcatc
     3541 tacaacgtca aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     3601 cagatccgtg gagtcaactt cccatccaac ggaccagtca tgcaaaagaa gaccctcgga
     3661 tgggaggcca acaccgagat gctctaccca gccgacggag gactcgaggg acgtaccgac
     3721 atggccctca agctcgtcgg aggaggacac ctcatctgca acttcaagac cacctaccgt
     3781 tccaaggtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaag
     3841 ccagccaaga acctcaagat gccaggagtc tactacgtcg accaccgtct cgagcgtatc
     3901 aaggaggccg acaaggagac ctacgtcgag caacacgagg tcgccgtcgc ccgttactgc
     3961 gacctcccat ccaagctcgg acacaagctc aacggaatgg acgagctcta caaggcctag
     4021 gataccggaa ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa
     4081 ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa
     4141 tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     4201 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     4261 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     4321 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     4381 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     4441 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     4501 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     4561 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     4621 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     4681 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     4741 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     4801 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     4861 caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt cgtctcgcgc
     4921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     5101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     5161 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5221 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5281 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5341 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5401 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5461 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5521 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5581 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5641 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5701 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5761 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5821 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5881 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5941 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6001 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6061 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6121 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6181 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6241 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6301 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7141 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7261 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7321 atgttgtgtg gaattgtgag cggataacaa tt
//
