LOCUS       sequence-441652-        5314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Mariner-2_PM Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Mariner-2_PM
FEATURES             Location/Qualifiers
     source          1..5314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(66..134,135..926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(284..303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1097..1685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1586..1605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1839..1856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1868..1884
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1868..1884
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2650..2874
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2863..2880
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2902..3615,3616..3618,3619..3621))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2905..3615,3616..3618,3619..3621))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2947..2968)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3296..3315
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3555..3576
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3622..3678)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3685..4284)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3765..3785)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      4281..4290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4304..4803)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4352..4371)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4745..4768
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5163..5180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5163..5179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5189..5207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5275..5314,1..65)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
       61 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      121 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      181 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      241 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      301 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      361 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      421 aattatgtag tgctgccata accatgagtg ataacacggc ggccaactta cttctgacaa
      481 cgatcggagg accgaaggag ctaaccgctt ttttgcgcaa catgggggat catgtaactc
      541 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgatacca
      601 cgatgcctgt agcgatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      661 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      721 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      781 ggtcccgcgg tattattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      841 tctacacgac ggggagtcag gcaactatgg atgaacgaaa gagacagatc gctgagatag
      901 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      961 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1021 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1081 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1141 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1201 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1261 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1321 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1381 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1441 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1501 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1561 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1621 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1681 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1741 acatgttctt tcctgcgtta tcccctgatt ctgtgggtaa ccgtattacc gcctttgagt
     1801 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1861 cggcacacag gaaacagcta tgaccgtctc ggttggcagt gactccgtct ctgggcccta
     1921 cagtggtacc tcggagatcg aacttaattc gttccaaaac cgtgttcgaa caccgaattg
     1981 ttcgaacacc gaatcaattt tccccataag aatgaatgga aatagcatta attggttccc
     2041 cacccaagga tgctcctatt atgggccatt tttacacaaa gtttacaata ttaaaataca
     2101 gtacagtaca taaaagcata caagaaacac agttataatt aatagaataa atgaacattg
     2161 aacttcactt tacctgtagt aaggagagtg gatggcttga gtggaaggtg gtgaggagga
     2221 ggaggaggtg tcagtgttgc ttggaagggg agtccccctc cataaaaacg tcgggtaact
     2281 gcccttctgg ggttctttct tctctttgcc ttttttctag cactaactgt gattcacttg
     2341 agggcctttt tactaaaaac ttatcaagag aggattgttt ctgcctccgt tttaagatgt
     2401 ttctgaaatg actcattgca ttatcattat aaaaatcgcc tgctctgctg gctactgcct
     2461 tatctgggtg atatttttct acaaaattgt gtaactcagc ccattttgca atcatttcct
     2521 ttatcagtga actaggggca ggatcccttc cctcctcctc ctctgaagac atgtcctctt
     2581 ttgccatctg ttgctgctca tcgtgaaggt gttggagttc ctctgtggct cgagggcgcg
     2641 cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
     2701 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     2761 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     2821 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
     2881 ctgatcagcg agcttaatta attacttgta cagctcgtcc atgccgagag tgatcccggc
     2941 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     3001 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     3061 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     3121 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     3181 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     3241 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     3301 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     3361 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     3421 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     3481 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     3541 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     3601 ctcctcgccc ttgctcacca ttggcccggg attctcttcg acatcccctg cttgtttcaa
     3661 cagggagaag ttagtggcat cgatggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     3721 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     3781 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     3841 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     3901 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     3961 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     4021 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     4081 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     4141 ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc
     4201 gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac
     4261 cgtgggcttg tactcggtcc ccatggtggc gttggctgca ggtcgaaagg cccggagatg
     4321 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
     4381 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     4441 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     4501 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
     4561 gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
     4621 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
     4681 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
     4741 tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta
     4801 cccggtagaa ttgctagcac cggttgtgta cggataaaca caaaaaacac taaaataaag
     4861 gaaattaacg aaaaagttaa cggaacgatg ttttgcgggt ctgcactggg cgagagctac
     4921 gcgtaaactg ggtcattcgg cctttgtctc ggtcgccctg gcctacgcgt ctcggcccaa
     4981 acaggttctt tccagtcgcc cgttcgacct ccgaatttct gttcggtgtc cggggcaaaa
     5041 aatggtcgga aatttcgttc gatcaccgaa ttgttcgagt tctggggcgt tcgatctccg
     5101 aggtaccact gtacaattga gagacgcaag acactgcgga tcgagacgca ttgcgtggtt
     5161 cgactggccg tcgttttaca acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5221 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5281 acccctattt gtttattttt ctaaatacat tcaa
//
