LOCUS       sequence-441654-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of piggyBac-1_XT Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-piggyBac-1_XT
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..39
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(51..638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(131..150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(660..689)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     724..742
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     751..768
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     752..768
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2421..2920
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2456..2479)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2853..2872
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2934..2943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2940..3539
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3439..3459
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3546..3602
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3603..3605,3606..3608,3609..4322)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3603..3605,3606..3608,3609..4319)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3648..3669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3909..3928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4256..4277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4344..4361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4350..4574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7431..7447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7431..7447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7459..7476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      7528..7559
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        7560..7662
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(7663..7731,7732..8523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAELGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7881..7900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             8524..8715
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg
       61 ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg
      121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      241 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa
      541 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      601 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc
      661 gaagaaaggc ccacccgtga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
      721 gtcaataggc gtatcacgag gccctttcgt tgtaaaacga cggccagtcg aaccacgcaa
      781 tgcgtctcga tccgcagtgt cttgcgtctc tcaattgtta acccttttag tgccatagga
      841 cgtagaatct acgtcctatg gcactatcgg gtttacaagc gctgcgctcg cttttaaagc
      901 agcgcagcgc ttgtaaagcc tgcacaaccc cctaggcaac gagcaaggaa ggacatactt
      961 acggatccgg tcccccagcc gcaccgatcc ggtccctcag ccaatgacag cagtggacac
     1021 gcattgatga cgtgtccact gctgtccctt taaatatcgc cgcccccgcc gtcgcctctt
     1081 cactcctgct gcgcacatgt gactgctgga gcccccctgc tgccttcctg ctgccttcct
     1141 gctggattgg tcgccctgat tgcctgcctg cattgtgtga gctgaactac aactctctaa
     1201 ccactgttta ttttgcttat ttctatctca actgtctaaa aatttttttt ttttttttcc
     1261 atttcttctt ctatctttgt cattattaca cttttgcaca catacacact tatttacaac
     1321 tttcccaagc acacacatac acttacattt ttttttacaa tttctttctt ttcttttctt
     1381 tctttctttg ctttctttgg cagtcttttt tttttactaa aactttattt ctgatcttgc
     1441 tctataatta tctgatttat ttggttgcat tttagtgttt ttttggcatt ttcataattg
     1501 atttctgttt tttaattatt ctattgcact gtaacttttt tattgctatt gggtgctgaa
     1561 tttgcagtga cgttggtgga tccagggctc tggccactga tttcattgca ataattgttc
     1621 ttctgttggt ttaggtggtt ttattggatt ttactgattt tatggtgttt tatctttgca
     1681 ttgttcttta ttgtgtattt agtgccccaa aaaaggttgc ttttgcagtg acattggtgg
     1741 atccagggct ctgaccaccg atttcattgc aattattgtt cttctgttgg tttaggtggt
     1801 tttattggat tttactgatt ttatggtgtt ttatctttgc attgttcttt attgtgtgtt
     1861 tagtgcccca aaaaaggttg cttttgcagt gacattggtg gatccagggc tctgaccacc
     1921 gatttcattg caattattgt tcttctgttg gtttgggtgg ttttattgga atttactgat
     1981 tttatggtgt tttatctttg cattgttctt tattgtgtat ttagtgcccc aaaaaggttg
     2041 cttttgcagt gacattggtg gatccagggc tctgaccacc gatttcattg caattattgt
     2101 tctttgattg cttttagtgg ttttattcca tttgatttca gttgttctag aaaggtccag
     2161 aggtgctaag ttcagattgt tctggtagtt tctattgcca aaggttaact cctggtcatc
     2221 tcttgctggc ttgagtttat caaaaggtta cagtgagttt ttttttctag cttctcctat
     2281 tacaagtgtt ctcctattac aagtgttgtc ttgcttgctt tttttttttt ttttgcttgc
     2341 ttgttttgac tttgcctgct gattgctaga tttgcagttg tcggtatatt tctggcacaa
     2401 ccggtgctag caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     2461 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     2521 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     2581 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2641 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2701 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2761 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2821 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     2881 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aacgccacca
     2941 tggggaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     3001 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     3061 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     3121 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     3181 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     3241 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     3301 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     3361 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     3421 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     3481 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
     3541 tcgatgccac taacttctcc ctgttgaaac aagcagggga tgtcgaagag aatcccgggc
     3601 caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3781 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4201 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4321 aattaattaa gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4561 ggtgggctct atggcttctg aggcgcgccc tcgagatttt gtgtttttat aggtaggtta
     4621 tggttctctg ggtttgttgg tggaataagg atttagcata tcagaatagt ggacttggca
     4681 tgttctaggt ttttatgcac aaggttgcag gcctccaaac ttagctgtgc tggcaaagtg
     4741 acgctgtttt ttatttattg agtttatcta ttctggcagg tccgtacttt acttataact
     4801 aggttataga tatatgtgat gaaaagtttg gtaaggtgtg tcatattatt ggcaaaagtt
     4861 tttttttttt agtaaagtag ggatttatgg tgtgtgattt tgttttttgc acaggttgtc
     4921 agaaaaacaa agtgtgcttg tagcttgtgg ttgtttagac ttgttcttgg gcattcagat
     4981 ttttgtgttt gagctatggt tattcaggct agttgcagtg tttctttggt tatggggtag
     5041 gacttatatt gtgctgttgg tatagcgaac tgaggtgctg ggtgactgac agggcgcatg
     5101 ataaaacttg tggtgtgtga tgaatcaatg agatgttttt atctctaaag ctgatgccac
     5161 aagtcctttt actggcacct caaaagcttg cctgtcagcg cagttatctg tgcatagttt
     5221 tgtatattac tttgtatttc tgggcagcat ttaactactg taagtgtagg ccttaagttt
     5281 tttgcacgac aaaggttctt gagcacagga tgacagatac attacggttt tttgtgcttg
     5341 tagtacaaat tgaatatcta ctgtatgttc tggttaggtt acacaagttg cagtggctct
     5401 ctaaatataa aaactttgtg taatactggt gtgccactgg tgactgctgc acggcacgta
     5461 atggtgcaca taattttatc tctgatgcta atgccccaag ctttattgtt ggcacctaaa
     5521 ttcctaacct ttagtgcaac ttctaaggac acttgcatca tttatgggcc aaattactat
     5581 ggattttttt ggcaaatatc cttctaaatg tggtgcagat tttgtcattt ctaaagtagt
     5641 gtaggaagaa gcaaaaaact ggtatcataa caccacagga aagatggaac tcaaatcaaa
     5701 gatgctccgc taaacagaaa taggtgagta aagtacaatt taggggttta tttgggggag
     5761 ggggtaactg tacagatggg ggggttaaag taaaaaaact gcaaggggtg catagagtgc
     5821 agccccaata ttatgcattt tcagggcatt tggcggccat gtacttatgt gcaaccagac
     5881 atatggggtg taattttatt cagggtaact tgctaattga tattgagcaa gttttttgat
     5941 agttgccatt gagattttgg ggagaaatct aactttatat ctgttttttc cttgatttca
     6001 gactaaagcg cagacttgta tagggaactg cagccacaat tttgcctgta gaaaacgaag
     6061 aatggtgtct ctaaatagct gaagttgtct atttttaatc aatgtatagt ttatatgggt
     6121 tttttcacag gtaaggggca attttgtctg aaaaactttg agatgtgcac ataaattgca
     6181 gcccccaaaa tttcaactga aattaccctt atgcattgcc cctgttttgg ggcatttggt
     6241 ggccacgtct ttatgtgcac ccatacatat ggggtatcgt tttattcagg ggaacttgca
     6301 gattgatgtt tagtaagttt ttggtagttg ccatggagat tttggggaga aatctaggtt
     6361 tgtatctggt ttttccttga tttctgacca aagcgctgac ttctgcaagg gactgcggcc
     6421 acaattttcc atgtagaaag agaagagtgg tgtctctgaa tagctgaagg tgtgcacttt
     6481 tcagaaatat atagtttgtg ggggttattt cacaggtagg gggggttaac actgaaaaac
     6541 tgcaggaagt gcacatagag cgcagccccc aaattttcaa ctgaaattgc ccttatgcat
     6601 tgcccctgtt ttggggcatt tggtggccac gtctttatgt gcacccatac atatggggta
     6661 tcattttatt caggagaagt ttgtctttca aatttgcctt ggttagaaaa tttttatgag
     6721 attttttttt gtcaaatcca catttgatca tgcgtccaag tttacgtttt agaaaaaaaa
     6781 aatgtcaaaa aaagctccaa atttcgcaat gcactgacaa aaggtatttg gcttttgagt
     6841 gaaaactaca ttgcacctag aaacctgaag gtctgtagtt tctaaagata ccaaacatga
     6901 ggggatattt tagatttaca tataagttat gctgcatcaa ctgttacaag cgcttttccg
     6961 ctttgttctg gtgtgatatt gtacaaagta ttgctttagt ttgggggtta cttctggaca
     7021 ggaactgtgg ggtaccacca catatttggt atcgttggac ttgggagtat cagggctttt
     7081 acaaacaaca aaaaaagtgt gtaaaattaa cttttctatg gaaaaaaaac tcaaaatata
     7141 cagaaatttt tcataatttt ttttttttta catatttcac ccaaaataca catcatatct
     7201 ccagaaaagt tataaaattt ggtatgtatg tcgaagccca attagtgatg aaaaaaacga
     7261 tatataattt ccctagtttc atggaggttt tcctaccaaa aaacattgtt aaagtgaatg
     7321 agtacaaaat gcttaaaaaa cgtctggcac tggggggaac tgaaatgacg aattcggctg
     7381 gcacttaaag ggttaagggc ccagagacgg agtcactgcc aaccgagacg gtcatagctg
     7441 tttcctgtgt gccgcttcct cgctcactga ctcgctgcgc tcggtcgtta ctgaccattt
     7501 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
     7561 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     7621 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagtat tcaacatttc
     7681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7801 ctggatctca acagcggtaa gatccttgag agtttacgcc ccgaagaacg ttttccaatg
     7861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7981 acagaaaagc atctcacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8041 atgagtgata acactgcggc caacttactt ctggcaacga tcggaggacc gaaggagcta
     8101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8281 gactggatgg aggcggataa agttgcagga tcacttctgc gctcggccct cccggctggc
     8341 tggtttattg ctgataaatc tggagccggt gagcgtgggt cccgcggtat cattgcagaa
     8401 ctggggccag atggtaagcc ctcccgcatc gtagttatct acacgacggg gagtcaggca
     8461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8521 taagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
     8581 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa
     8641 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac
     8701 ggtgaaaacc tctgatgagg gcccaaa
//
