LOCUS       sequence-441655-        4926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srz-45 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY133
FEATURES             Location/Qualifiers
     source          1..4926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             601..621
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             646..666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             688..708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2517..2539)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2639..2658
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2736..2754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2822..2926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2927..2995,2996..3787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3145..3164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3958..4546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4447..4466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4834..4855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4870..4875,4876..4893,4894..4900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4908..4924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(4913..4926,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tttcacacct
      181 tgaagagcgg cagccgacgt tgtaacgatt gtttgtaggc tgaaaatatg ttcacaagaa
      241 gtgcgaagct ttattctaaa attgtttatt ttgtttacat gccatatttt tttgaaagct
      301 tttccgatca aacataactc ggcgcacatt aaaaattaat caaattgaat ttactagtat
      361 ctacgtatgt atttgcaaat acttggaaga ctacacacgt caaacacaca cctggttgca
      421 acattactta attttttcag tttttcagtt tatgacttcc agcccgggat tggccaaagg
      481 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
      541 ggagggtacc ggttttccca gtcacgacgc gtggtaccga gctcagaaaa aatgaccgct
      601 ccaaagaaga aacgcaaagt accgagctca gaaaaaatga cggcacctaa aaagaaacgc
      661 aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc gtaaagttcc acgcgtcatg
      721 ggagtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagcgcgct
      781 gtctccaagg gagaggagct catcaaggag aacatgcaca ccaagctcta catggaggga
      841 accgtcaaca accaccactt caagtgcacc cacgaagggg agggcaagcc ctacgagggc
      901 acccagacca acaggattaa ggtggtggag ggaggccccc tgccgttcgc attcgacatc
      961 ctggccacct gctttatgta cgggagcaag accttcatca accacaccca aggaatccca
     1021 gacttcttca agcaatcctt cgtaagttta aacatgattt tactaactaa ctaatctgat
     1081 ttaaattttc agcctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     1141 gtgcttaccg ttacccagga caccagcctc caggacggct gcttgatcta caacgtcaag
     1201 ctcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1261 gaggccagta ccgagaccct gtaccccgct gacggcggcc tggaaggcag atgcgacatg
     1321 gccctgaagc tcgtgggcgg gggccacctg cactgcaacc tgaagaccac atacagatcc
     1381 aagaaacccg ctaagaacct caagatgcca ggagtaagtt taaacatata tatactaact
     1441 aaccctgatt atttaaattt tcaggtctac tttgtggacc gcagactgga aagaatcaag
     1501 gaggccgaca atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     1561 ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa gtaagcgccg
     1621 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     1681 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     1741 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     1801 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     1861 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     1921 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     1981 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     2041 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     2101 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     2161 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     2221 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     2281 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     2341 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     2401 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     2461 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2521 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2581 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2641 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     2701 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     2761 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4681 cagccgaacg accgagcgca gcgagtcagt gagcgatgaa gcggaagagc gcccaatacg
     4741 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4801 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4921 acaatt
//
