LOCUS       sequence-441664-        8333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pdf-1 fluorescent neural reporter driving nuclear CyOFP1 expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY114
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3692..3712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3734..3754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5924..5946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6046..6065
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6143..6161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6229..6333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6334..6402,6403..7194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6552..6571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7365..7953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7854..7873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8107..8124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8241..8262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8277..8282,8283..8300,8301..8307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8315..8331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8320..8333,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aaaggttgct
      181 ttaccgagac gtgacgggac actccttatc agcgatctaa ctttgtatgg aacttttcac
      241 aactctggat gctggcgatc gtaaatcgta ctgctccaaa tgtctagaac tttaaagaca
      301 ataaaaagcc gcaaaagtat ttgtaaaaaa tcaaagaaaa tagtttatat cgaaaattaa
      361 gtatcaagac ctttttagaa gaatggcctt gtatgatttg tgagttgaca tcttctgatt
      421 ctaaagatgc gtaaactttg aaactgaata aacccctctt tcatattcaa aagacctaaa
      481 ttcttgtaac acaacatcca aaattgtacg tgacaactgg aataatgact gattttcact
      541 atagcctcct atttcggttg ttttgtttat ttgtttattc attaatattt tcataataat
      601 gaaattatcg agggtcttta acacttttga gcacatagag ctctcatgtg acgtcaaagt
      661 tggttttgaa acctcgtgtg gtacttctta ttcttttttt cttcggtcga tcggaacaat
      721 tcaattaaaa ctaatgcaca tacatcccct tttctaccac tccaccatct tcttcttctc
      781 tttttcttct tccaacacac gaagattctg attctgattc catcatgacg tgttgatgaa
      841 agaaggaaac caagtagtag cagaagtaaa aagaggagga aaaaagtttt ttggaaggaa
      901 aaaggaaaaa gcaatttgat tttggagaag attcttcccc ttctcactac catcccacaa
      961 aatttcttgc ggaggcttaa tcttttcctg caaaataaga taaattggat gcgcgaattc
     1021 cgtgtcattt gcacaagggc aacagaaatc gagtaccaag agttcataaa cttttcttgt
     1081 ttcaatgtaa aaacaaatga acatttagac agaaattcca ataaaccacc aaatttgaat
     1141 ttccttaaac atctcatata tggacacggc taacaaggtc ctcttgcaaa tataataggt
     1201 ttcattcgga agtcatagga cgagctgaaa gggttccctt ggaagacaaa aattattaaa
     1261 gactaaaaac gactgctcca ggtaggtaga caggggaagg catgtcaaag tgattgccta
     1321 taacggaagt cctagaaaat gtaaaatatt attctactgt tttgttttat ttcattcgat
     1381 tttcaaacac ttgtgaagga atctcgaagt aaaattttct cgaaacggag gttgtagtac
     1441 agttggcaaa aagtgagtaa ctgtcttcag tagctgtatt aggctagata ataaactaag
     1501 ctttataata tctggtcacc caaattaaac aagattaaac gaagcacaag aaatggagaa
     1561 aaagcaagta aagactcagg accaaaggaa ttccccgcga aactagcaga ccaaaacaat
     1621 atatctatct ttctctctag taatttaaat ttcatctgaa acttgtacat gacctctctt
     1681 ccttctccac gcctacttcc tccttttttt ttcattgagg cttcccgagt tgacgaagaa
     1741 tcatcatgtc cattaatttt acattaaata ttaaaaatgg gaaaacggac atttgtatct
     1801 cgtcatttat ctttcggaaa ctcccagata gtattgctcc ttgatcttct agaaaacttc
     1861 aagctcaact aaatttaccg tactcacggg tacggtagct gagaattttc atgtatgaat
     1921 gttaggaccg cccatcgaga acattcccat aacctgtcca gctacaataa tcatcttgaa
     1981 tgctcaaaaa gcgccccttt tttcaaacgg tttaaaaagt ttaaaaagtt caaaaaatgg
     2041 ttttaaatta tttttccttg agaatttgac aaagaataag gttttataaa aaactaattt
     2101 tgttttcaaa acaagtaact tctaaaggaa atcctctttc tccagtattg aatattccag
     2161 tgactctata tttctgagaa tttatttttg accttcgaca ttttcaaaat attttctagt
     2221 ttctcaaggc gttcttttgt atcaataagc caaacgtgtg taagtgtgta agtggaaggc
     2281 aaaatatgtt ggtgtagaaa atactatacc tcggcacgag ctgactagac tcaggtaaca
     2341 aaaagctgta aaaacaagtg aattttgcta cgcaaacctg tttaagactt tctgaattaa
     2401 acactctcac caaatatttt ttttaagatt gaagatactt gacgatctct ttttgaaact
     2461 ttcataaaac ttcttgttca taaaatctat tgtacaccca aaataaaatc tctgtttatt
     2521 tcatcattgc tcactaaaaa ctctcccatt gatttgtcaa tgctttgatt ctagttaatt
     2581 agacttaaac agaagaggca ggggaaaagg aaaaaaggat tagggaattc tttgaaaccg
     2641 ctttgtttgt ggtcagagtt cgtgtcaatc tgaaaataat tcccatgaaa atgacggaaa
     2701 atgtgaagaa cggagaatta aatttcacct gtaattttta atggtatata cgacatgaat
     2761 tatgcatagc aggtttgttg attttttcaa ggtctcgcct ctaggaattt tgtttcaaga
     2821 attgaacaac tttgtagagg aaaaacatgg aaacagtaga aagctagaat gttttaatga
     2881 tcatatatat cttgtaaatg taaaaagttt agactttcaa gaaagcaaat tttctactca
     2941 aaactcaaaa tatgaatacg atccggtggc agatctttgt tgaattgttg aaatgttgga
     3001 tactttttgt ttcttccttg tgattttcca ggttatacaa actttcctga tttttgaaga
     3061 gtttatcgat aacatgatgt attttcgaaa gtttgttgaa actcatcaaa gtttaccgca
     3121 gtcgattgaa aattgataag ttcatggcca aaaactgtgc aaccttcctc ctttgaagat
     3181 ttcaagtctt cagctttcct tgtctggcat ggagcactcc tattcctgag gtcgcagttg
     3241 attttttgca agtttttcct caatctcatt aagattatcc cactagtgac gtcgacatga
     3301 ttcagacatt tttctttctt ccgaattcaa acttttcaat ctccttttct ctctatttct
     3361 ctcaaatttc cgtcatattt tcacctcggg ttccggggcg ttttaaaagt gggcgtgttg
     3421 ctccctcttg gtgcgcgggt cgaagagagg cggagtctaa aactagagag atctgtctct
     3481 ctctcggttg tgctcattca ttctattcga cttgtgcctc acagccaagt gaacaagctt
     3541 ttccagctct tcctcttcct tcttcactca acgtcaaccc gggattggcc aaaggaccca
     3601 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg
     3661 gagggtaccg agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga
     3721 gaagatgact gccccaaaaa agaaacgtaa ggtcccgcgg gtaagtttaa acagttcggt
     3781 actaactaac catacatatt taaattttca gcgcgctgtg tcaaaaggag aagagcttat
     3841 taaggagaat atgcgttcca agctctacct cgagggatcc gtcaacggac accaattcaa
     3901 gtgcacccac gagggagagg gaaagccata cgagggaaag caaaccaacc gtatcaaggt
     3961 cgtcgaggga ggaccactcc cattcgcctt cgacatcctc gccacccact tcatgtacgg
     4021 atccaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt
     4081 cttcatcaag tacccagccg acctcccaga ctacttcaag caatccttcc cagagggatt
     4141 cacctgggag cgtgtcatgg tcttcgagga cggaggagtc ctcaccgcca cccaagacac
     4201 ctccctccaa gacggagagc tcatctacaa cgtcaaggtc cgtggagtca acttcccagc
     4261 caacggacca gtcatgcaaa agaagaccct cggatgggag ccatccaccg agaccatgta
     4321 cccagccgac ggaggactcg agggacgttg cgacaaggta agtttaaaca tgattttact
     4381 aactaactaa tctgatttaa attttcaggc cctcaagctc gtcggaggag gacacctcca
     4441 cgtcaacttc aagaccacct acaagtccaa gaagccagtc aagatgccag gagtccacta
     4501 cgtcgaccgt cgtctcgagc gtatcaagga ggccgacaac gagacctacg tcgagcaata
     4561 cgagcacgcc gtcgcccgtt actccaacct cggaggagga atggacgagc tctacaagcc
     4621 taggataccg gtgggtgaag acccaccaaa gccatctgcc aagggagcca agaaggccgc
     4681 caagaccgtt acgaagccaa aggacggaaa gaagagacgt catgcccgta aggaatcata
     4741 ctccgtctac atctaccgtg tcctcaagca agttcatcca gacactggag tttcctccaa
     4801 agccatgtct atcatgaact cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc
     4861 atcccgtctt gctcactaca acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc
     4921 tgtccgtctg atccttccag gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc
     4981 cgttaccaag tacacttcca gcaagtagga attccaactg agcgccggtc gctaccatta
     5041 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
     5101 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
     5161 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     5221 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
     5281 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     5341 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     5401 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     5461 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     5521 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     5581 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     5641 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     5701 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     5761 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     5821 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     5881 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5941 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6001 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6061 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6121 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6181 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6781 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6841 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6901 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6961 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7021 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7081 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7141 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7201 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7261 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7501 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7561 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7621 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7681 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7741 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7801 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7861 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7921 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7981 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8041 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8161 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8221 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8281 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
