LOCUS       sequence-441665-        7825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-31 fluorescent neural reporter driving nuclear TagRFP-T 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY143
FEATURES             Location/Qualifiers
     source          1..7825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3429..3449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3474..3494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3516..3536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5416..5438)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5538..5557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5635..5653)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5721..5825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5826..5894,5895..6686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6044..6063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6857..7445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7346..7365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7599..7616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7733..7754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7769..7774,7775..7792,7793..7799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7807..7823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7812..7825,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tatccgagca
      181 gaaagatagt cgatttcata acatggtaat acgattagcc ggaacagaaa ataaatatgt
      241 caagatttca taaatatcaa acccacgcca aacggtttat tatattaaaa acttattcat
      301 gatatcattt tggacatata taaggtcatg aaaaagttgt aaaagttacc gataatcttt
      361 tcatagaaac attttggaat taaactcttt tttaatttat ttttgtattt taaactattt
      421 tcagcgaaat gaatcttgtc gcttcaatgc gagcaaatca ttggtagctg aaagggtggt
      481 tgtagctgta acaagacgta gtcatgaaaa agtggaagct agttcttgat ggttgttctt
      541 gatctaccac ggctccgcat ttttcccata ctaaaaatta cgaaaaagcc gaaaatgtca
      601 aagtatcgaa aattttgaat cggcaatttc tgaaagaact ggttttatct tccagttcgg
      661 caattgtcga attatcggaa aaaattctaa tttccagaat gaaagaactt gactttcaga
      721 ctatagtaca caggaaattc tcctcatttt tgagtttatt tttttaaata attttcgaac
      781 tcgataattg tcaaattttt gaattggata tttgccaaaa tgccttcgcc aaacattttt
      841 taactacgcc caattattga attgccaaaa aatggaattt aattttaatc ctcccaaatt
      901 tttgtcattt ttcagtgttt ttagtttccg gaagtatcat catcatcttt tgcatatcct
      961 ctcattttcg tgtccatctc tctttttttt tcttccgtcc aatgtagcat tagcctcttc
     1021 cagaaacgag agaaacccca gcagcagtag cagagtaggc agcagaagct cttctcttct
     1081 gcttcagctt ctgcgcctct ccgtttttct gtctgcgtgt gtgtatgtgt gtcctaattc
     1141 agcgtgatgt cgtcgccgcc ggcctattga tcttcgcacc gcttctggtg tgccccccat
     1201 gtgtgtgtgc atgtgctctg cgtctcccgg ctccccgtct cctaatgcat gtattttcta
     1261 gtgggggtcc cccgcccgct tcttcttccc gcgcgcgagc gccatgactg ctgcttctcc
     1321 gccccagacg acgacgattg ttttccaaaa atgaaacact tttgtgttgc tcatacccca
     1381 tttcctccaa aattagcatg ccttccaact ccactgcccg ttttttcctt ctttctcccc
     1441 cggcctctca tagttctcac acatcaaata ttttatattt tatatttata aaccccgaac
     1501 gcgttccttc ctcctctccc catatttttc ccttttttgg aatattttaa aagcttgtgt
     1561 tttcttttta tcgaaaaaaa cgcaaaatta cagccttcgc gtagaggcta actagtaatt
     1621 tccgtgtagc aaaagagcaa cagagccata atcatagaga gaatattcaa tttcgggtgc
     1681 atttctaatg aaacaaagta ttccggaggg caaaaaaggg agaatatttg caatttgtat
     1741 ttctcttcca atcaccacat ttttatttcc tcccacactc ctccccgcaa aatgacattt
     1801 tcggattgat tttgtgtgtg ttttgagccc gtttccattt atatttggaa tttttttggt
     1861 ttgcaatttt tcctgagaaa atcaaaaaaa tattgaaagt ttccaattct gtatgatttt
     1921 ttttttttca aaaaatgata cattcagttt cttcttgaac ttctaaaaaa tcaaaattta
     1981 ttttgcgaaa taaaaattaa acttcatcaa attttgtgca tgttgttgga aaaaaatttg
     2041 aagaaatata actttaatca acaaataaaa aaatagtcca aatttcgaga aaatttaaaa
     2101 aattgttctt ttaaaacatt tttgtaattt ttttcgagca atcgtgaaaa aacatttcaa
     2161 ttttttttta atttttactt aaaaattcaa atattgataa aattgtgata attactttga
     2221 caattaacag aaaaaaggca ttagacaact gtttttttta aacatttttt aaaaactttt
     2281 tttggcacgt cccgtttgac cgcgtggaat atgtcgaaat ataagtggtc aaaaaggaaa
     2341 tgtatgtact gcacttttgt ttataccacg aatcgtaatc aggttatatg accattttta
     2401 ttaaattact gcgccaactt ggtcagaaat ctctatgaat caatgttatg tatttcattt
     2461 tctctcccag ggatttcacc cccacaccac cactgacatc atctgaacta tttatttaca
     2521 tttactccaa gcacaccaat gaaacataca catatcttat ttttagattt accctctttg
     2581 gatagcaaat atacccattc ttaatccata atatttagca actaacataa gctcccacaa
     2641 ttatttaact tgatttttga cgattttgca gcctgataca aatggtcaca acattttgta
     2701 gctaggatta ttcaaatcct ttgaaatatg aaattgtcta gccataactt ttagaagaaa
     2761 tttttctgac aatctgctcc taaaaagcga ttgtgttgga ccatctgatt tttcactcca
     2821 caactttcgc ctaaccgcat ggataaatcc attaaacttc ccgttttctc agacattcta
     2881 catatattct ttgaacttta atccatgtct agacacaaaa aattattcct taaaaatttc
     2941 aaaaacaagt caatttttca aaattttgtg cgcaaaaaga aagaagacta acacagcgca
     3001 caagaaaata catatcagta gttcatgccg ataatccgac agcttctaat tcggttttca
     3061 catttttttc tcgccttttg gctccttgtt tgttattttt cctctgatct ctatgcctct
     3121 ctctccctct ctacttctct cccggattcc cattttcgtt cttccgttct ttttttttct
     3181 cccccatttt tttcgccttt ttatcctagt ttatattatg cattattttg cctttctcgt
     3241 acgtccgtcc ccgccgctcg tttcaattca atttcaagtc tccttgttat cccgggattg
     3301 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     3361 ggacccttgg agggtaccgg ttttcccagt cacgacgcgt ggtaccgagc tcagaaaaaa
     3421 tgaccgctcc aaagaagaaa cgcaaagtac cgagctcaga aaaaatgacg gcacctaaaa
     3481 agaaacgcaa agtaccggtc gccgagaaga cagccccaaa aaagaagcgt aaagttccac
     3541 gcgtcatggg agtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     3601 agcgcgcttc caagggagag gagctcatca aggagaacat gcacatgaag ctctacatgg
     3661 agggaaccgt caacaaccac cacttcaagt gcacctccga gggagaggga aagccatacg
     3721 agggaaccca aaccatgcgt atcaaggtaa gtttaaacat atatatacta actaaccctg
     3781 attatttaaa ttttcaggtc gtcgagggag gaccactccc attcgccttc gacatcctcg
     3841 ccacctcctt catgtacgga tcccgtacct tcatcaacca cacccaagga atcccagact
     3901 tcttcaagca atccttccca gagggattca cctgggagcg tgtcaccacc tacgaggacg
     3961 gaggagtcct caccgccacc caagacacct ccctccaaga cggatgcctc atctacaacg
     4021 tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagatcc
     4081 gtggagtcaa cttcccatcc aacggaccag tcatgcaaaa gaagaccctc ggatgggagg
     4141 ccaacaccga gatgctctac ccagccgacg gaggactcga gggacgtacc gacatggccc
     4201 tcaagctcgt cggaggagga cacctcatct gcaacttcaa gaccacctac cgttccaagg
     4261 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aagccagcca
     4321 agaacctcaa gatgccagga gtctactacg tcgaccaccg tctcgagcgt atcaaggagg
     4381 ccgacaagga gacctacgtc gagcaacacg aggtcgccgt cgcccgttac tgcgacctcc
     4441 catccaagct cggacacaag ctcaacggaa tggacgagct ctacaaggcc taggataccg
     4501 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
     4561 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
     4621 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     4681 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     4741 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     4801 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     4861 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     4921 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     4981 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     5041 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     5101 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     5161 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     5221 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     5281 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     5341 ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     5401 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5461 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5521 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     5581 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     5641 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6541 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7621 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7681 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7741 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7801 gtggaattgt gagcggataa caatt
//
