LOCUS       sequence-441668-        5703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ser-2(promCE) fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY119
FEATURES             Location/Qualifiers
     source          1..5703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1068..1088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1110..1130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3294..3316)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3416..3435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3513..3531)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3599..3703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3704..3772,3773..4564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3922..3941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4735..5323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5224..5243
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5477..5494
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5611..5632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5647..5652,5653..5670,5671..5677)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5685..5701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5690..5703,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg cacacacaca
      181 cacaaattca aatactcgag ccacgcaagc tgagcacaaa tcagtttgca ctggtgcatt
      241 gttggtctat aaaacttcac cattgactgg agagcgaccc gcacaacgga aaaactatag
      301 aatccctcta atcccgccta ctggtgctct ttgccatttt cgtgacaaag attattcttc
      361 tgaatcctcc tcctcctcct ctatccatcg tccagttctt cgcttcattc ttgttcattc
      421 ttgtcgccac gagaaaaatt tgcctctttt ctcttttctt tttattctat ctcgaaattc
      481 tgtttggtgt gaggggactc gacaatcccc cccaagcacc tcttaggacc actgtcccgt
      541 gttgagtcct gtcctattat tattttcatt atttctattc ttaatctcta aatgacgagg
      601 agccacattg ctccagctcc agaaaacaga cgcggctttt tcgggcaaca aatgtccaca
      661 cattcaacaa cgccgtcctt atagatccta cgatatattc ttgattacgc tttaacgaaa
      721 gctaaacaag ttctgtttta acagaaaagc agtgaaaaat aggagaaaaa gaagaagccg
      781 tgggcgtggc ttgccattca cacagacttt tcggaagcgt tagataactg ccctaactaa
      841 agggggactc ccccgtcttc ctgcttccgt cgcttctttc tctcatttgg ccaactatcg
      901 acagtaaatt gtgcgagtgc gtcagagtct caaggcagcc tcaagtaatt tgccccggga
      961 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1021 ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gaccgctcca aagaagaagc
     1081 gcaaggttcc agtcgagaag atgactgccc caaaaaagaa acgtaaggtc ccgcggatgt
     1141 cagagcttat taaggagaat atgcatatga aactgtacat ggaaggaacc gtggacaacc
     1201 atcactttaa gtgcacttcg gagggggaag gcaaaccgta cgagggcacc cagaccatgc
     1261 gaatcaaggt ggtcgaggga ggcccactgc ccttcgcctt cgacattctg gtaagtttaa
     1321 acatatatat actaactaac cctgattatt taaattttca ggctactagc ttcctgtacg
     1381 gcagcaagac ttttattaat cacacccagg gcatcccgga cttcttcaaa cagtcgttcc
     1441 ctgagggctt cacatgggag cgtgtgacca catacgaaga cggtggcgtt ctcaccgcta
     1501 cccaggacac cagcctccag gacggttgcc tcatatataa tgtggtaagt ttaaacagtt
     1561 cggtactaac taaccataca tatttaaatt ttcagaagat ccggggagta aacttcacat
     1621 ccaatggtcc ggttatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
     1681 atccggctga cggaggcctg gaaggtcgaa acgacatggc cctgaagctc gtcggcggta
     1741 gccatctcat cgcaaacgct aagaccacat atagatccaa gaaaccggta agtttaaaca
     1801 tgattttact aactaactaa tctgatttaa attttcaggc taagaacctt aaaatgccgg
     1861 gagtctacta tgttgactac agactggaac ggatcaagga ggccaacaac gagacctacg
     1921 tcgagcagca cgaggttgca gttgccagat actgcgacct cccgagcaaa ctgggtcaca
     1981 agcttaatcc taggataccg gtgggtgaag acccaccaaa gccatctgcc aagggagcca
     2041 agaaggccgc caagaccgtt acgaagccaa aggacggaaa gaagagacgt catgcccgta
     2101 aggaatcata ctccgtctac atctaccgtg tcctcaagca agttcatcca gacactggag
     2161 tttcctccaa agccatgtct atcatgaact cttttgtcaa cgatgtcttc gagcgtattg
     2221 ctgctgaagc atcccgtctt gctcactaca acaagcgttc cacaatctca tcccgcgaaa
     2281 ttcagaccgc tgtccgtctg atccttccag gagagcttgc caagcacgcc gtgtctgagg
     2341 gaaccaaggc cgttaccaag tacacttcca gcaagtagga attccaactg agcgccggtc
     2401 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     2461 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     2521 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     2581 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     2641 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     2701 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     2761 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     2821 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     2881 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     2941 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     3001 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     3061 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     3121 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     3181 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     3241 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     3301 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     3361 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     3421 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     3481 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     3541 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4381 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4861 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5521 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5581 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5641 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5701 att
//
