LOCUS       sequence-441672-        9225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAC-tagged VPS35L in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Laulumaa S, Kumpula EP, Huiskonen JT, Varjosalo M
  TITLE     Structure and interactions of the endogenous human Commander 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 
            10.1038/s41594-024-01246-1. Epub 2024 Mar 8.
  PUBMED    38459129
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01246-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-06"; volume: "31"; issue: "6"; pages: "925-938"
COMMENT     Sequence Label: MAC_C_VPS35L
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3728..3752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3799..3822
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3859..3882
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3913..3939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3913..3939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3940..4287,4288..4290,4291..4899)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4943..4960)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4949..5173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5306..5325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5457..5504
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5512..6534
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5512..6534
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6664..6785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6701..6720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6755..6774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6834..6850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6834..6850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6847..6869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6858..6874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6882..6888,6889..6906,6907..6912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6927..6948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7065..7082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7236..7824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7316..7335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7995..8786,8787..8855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8618..8637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8856..8960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9035..9054)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catggccgtc
      841 tttccttggc actccaggaa taggaactac aaagctgaat ttgcatcatg ccgactggag
      901 gctgtaccat tggagtttgg ggactatcac cctctgaaac ccataactgt cacagagtca
      961 aagacaaaga aagtgaaccg gaaaggaagc acttcttcca cgtcctcctc ctcctccagc
     1021 tccgtggtgg acccgctgag cagcgtcctc gatgggactg accccctctc catgtttgca
     1081 gccactgctg accccgcagc cttggcagct gccatggaca gctccagaag gaaacgtgat
     1141 agagatgata actccgttgt aggatcggat tttgagcctt ggaccaacaa acggggagaa
     1201 atccttgccc ggtacaccac taccgaaaag ctgtctatta atctgtttat gggatctgaa
     1261 aaaggcaaag ctgggactgc cacattggca atgtcagaga aggtgcggac ccggctggag
     1321 gagctggatg actttgagga gggttcccaa aaggagctgt tgaacttgac tcagcaggat
     1381 tacgtgaacc gcatagagga gctcaaccaa tcgctgaagg atgcctgggc ctcagaccag
     1441 aaagtgaagg ctctaaaaat agtcatccag tgttcaaagc ttctttcaga caccagtgtt
     1501 attcagttct acccaagcaa atttgtcctt atcaccgaca tacttgatac atttggaaag
     1561 ctcgtgtacg agcgcatctt ttccatgtgt gtggatagcc gcagcgtctt accagatcac
     1621 ttttctccag agaatgcaaa tgacacggcc aaggaaacat gcctaaattg gtttttcaag
     1681 attgcctcca tcagggaact cattccaaga ttttacgtgg aggcatccat cctgaaatgt
     1741 aacaaattcc tctccaaaac gggaatttca gagtgcctgc cccggttgac atgcatgatc
     1801 agagggatcg gagacccact agtgtcggtg tatgcccgtg cctacctgtg ccgggtggga
     1861 atggaagtgg ccccacatct caaagaaacc ctaaataaga acttttttga cttcctcctt
     1921 acgttcaaac agattcatgg ggatacggtc cagaaccagc tggtggtcca aggagtggag
     1981 ctcccatctt acctcccctt gtacccgcct gccatggact ggatcttcca gtgcatctcc
     2041 taccatgccc ccgaggctct gctgaccgag atgatggaaa ggtgtaagaa actaggaaac
     2101 aatgccttgc tgttgaattc tgtgatgtct gccttccggg ctgagttcat cgccacaagg
     2161 tctatggatt tcattggcat gattaaagag tgtgatgaat ctggtttccc caagcatctt
     2221 ctttttcgat cactgggatt aaacttggcc ttggctgatc ctcctgagag tgaccgactt
     2281 cagattctca acgaagcttg gaaagtcatc actaagctga agaacccaca ggactacatt
     2341 aattgtgccg aagtgtgggt ggaatacacc tgcaagcatt tcacgaaacg agaggtgaat
     2401 accgttttgg cagatgtcat caagcacatg actccagatc gtgcatttga agattcctac
     2461 ccccagcttc agttaataat taagaaagtt attgcccact tccatgactt ctcagttctt
     2521 ttctcagtgg aaaaatttct gccgtttctg gacatgttcc aaaaagagag tgtgcgggtg
     2581 gaggtttgca aatgcatcat ggacgccttt atcaagcatc aacaagagcc caccaaggac
     2641 ccggtcatct tgaatgccct tttgcatgtt tgcaagacca tgcatgactc tgtgaatgca
     2701 ctcactcttg aggatgagaa aagaatgctg tcatatttga ttaatggatt tataaaaatg
     2761 gtttcctttg gccgtgattt tgaacaacag ctgagttttt atgttgagtc caggtcgatg
     2821 ttttgcaatc tggagcctgt tcttgtgcag ttgattcata gtgtgaaccg gttggcaatg
     2881 gagacaagaa aagtaatgaa aggaaatcat tccagaaaga cagctgcatt tgtccgggcc
     2941 tgtgttgcct actgcttcat caccatcccc tccctggcgg gcatcttcac acgtctcaat
     3001 ctctacctgc attctggtca ggtggccttg gccaaccagt gcctctccca agctgatgct
     3061 tttttcaaag ccgctataag ccttgttccg gaagttccaa agatgattaa tattgacggg
     3121 aagatgcggc catcggaatc gttccttctg gaattcctct gcaatttctt ttctacttta
     3181 ttaatagttc cggatcatcc tgaacatggg gtcctgtttc ttgttcgaga gcttctcaac
     3241 gtgatccagg actacacctg ggaggacaac agcgatgaga aaatccgcat ctacacctgc
     3301 gtcctgcatc tcctctccgc catgagccag gagacgtacc tttaccacat agacaaagtg
     3361 gactccaacg acagcctcta cgggggagac tccaagttcc tggcagaaaa caacaagctg
     3421 tgtgagacgg tgatggctca gatcctagag catctgaaaa ccctggccaa ggacgagacc
     3481 ctgaagcgcc agagctcgtt gggcctttcc ttctttaaca gcatcttggc ccatggggac
     3541 ctacgcaaca acaagctcaa ccagctctcc gtcaacctgt ggcacctggc acagaggcac
     3601 ggctgtgcag acaccaggac catggtgaaa acgctagaat acatcaagaa gcaaagcaaa
     3661 caaccagaca tgactcatct gacggagctg gccctcagac tccctctgca aacaaggacc
     3721 tggatccacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     3781 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     3841 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     3901 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg
     3961 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca
     4021 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg
     4081 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac
     4141 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac
     4201 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc
     4261 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc
     4321 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc
     4381 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa
     4441 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg
     4501 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac
     4561 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa
     4621 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc
     4681 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg
     4741 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc
     4801 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc
     4861 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg
     4921 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4981 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5041 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5101 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5161 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5221 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5281 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5341 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5401 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     5461 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     5521 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5581 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5641 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5701 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5761 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5821 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5881 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5941 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     6001 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     6061 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     6121 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     6181 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     6241 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     6301 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6361 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     6421 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6481 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     6541 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6601 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6661 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6721 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6781 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9001 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9061 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9121 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     9181 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
