LOCUS       sequence-441673-        5894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-46 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY146
FEATURES             Location/Qualifiers
     source          1..5894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1259..1279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1301..1321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3485..3507)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3607..3626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3704..3722)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3790..3894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3895..3963,3964..4755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4113..4132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4926..5514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5415..5434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5668..5685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5802..5823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5838..5843,5844..5861,5862..5868)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5876..5892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5881..5894,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcgaacact ttagtagctc
      181 tcctgaattt ttgaggatat ttctgaagtc tatcaaattt taaacaattt actgtcaaaa
      241 aattccagat ttttccataa gttctaaatt gttttcaaaa gtttctaaaa gtttccaaaa
      301 ctttatagaa ggttccagaa ttatcacaga gtttttcaga attttccaga attttctaaa
      361 ctgaatttcc tgaatttcaa agaatatttc taaagccttt cgagtttttg aaaatttaca
      421 tgaaattaca gatttaacca tacgttttaa attttttttc aagagtttcc caaaatttcc
      481 aaaagtttca aaacattcga aaaaattttt aaaaatttct caaagtatcc agaatgaagt
      541 tgctagaagt ctctcggatt ttcagaattt ttcagaagtt tccaaatttt tccaagattt
      601 tccaaaaccc aactcaatat ttaaaaactc atttcttggt ctacagtaat tctacaatac
      661 tcctacagta cccctagagt acagatactt ttagaaattt aaggagcata ttctattttt
      721 cagttgaaaa ttgcagtttc acgaatatta ttctgtatta ttccacaaag tggacaaatt
      781 tacttgataa aaaccaaaaa tatatataca ttcgaaattc attttctcta tttggttctg
      841 aaactccatt tagttaaaat ctaaaatatt ccccaaactt aaaatcattc caactaaatg
      901 tgatagttgt acatgcggca aatgacaaaa acgatgaaaa atagaaagaa agaaagatgg
      961 gtcgtagaat ggaatacatc aaaagagctt aaaagtgtgt gctcattttg ggggtcctgg
     1021 tcctccaatc cgtgctccac acggaattat ttgttcttct tcttcttctt cgtcgctttt
     1081 cttcatcttc aaaagtgtct cctcaacatc cttcgtttct tcgcaaaaac aagattttag
     1141 gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
     1201 gaacattttc aggaggaccc ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc
     1261 aaagaagaag cgcaaggttc cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt
     1321 cccgcggatg tcagagctta ttaaggagaa tatgcatatg aaactgtaca tggaaggaac
     1381 cgtggacaac catcacttta agtgcacttc ggagggggaa ggcaaaccgt acgagggcac
     1441 ccagaccatg cgaatcaagg tggtcgaggg aggcccactg cccttcgcct tcgacattct
     1501 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc aggctactag
     1561 cttcctgtac ggcagcaaga cttttattaa tcacacccag ggcatcccgg acttcttcaa
     1621 acagtcgttc cctgagggct tcacatggga gcgtgtgacc acatacgaag acggtggcgt
     1681 tctcaccgct acccaggaca ccagcctcca ggacggttgc ctcatatata atgtggtaag
     1741 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagaaga tccggggagt
     1801 aaacttcaca tccaatggtc cggttatgca gaagaaaaca ctcggctggg aggccttcac
     1861 cgagacgctg tatccggctg acggaggcct ggaaggtcga aacgacatgg ccctgaagct
     1921 cgtcggcggt agccatctca tcgcaaacgc taagaccaca tatagatcca agaaaccggt
     1981 aagtttaaac atgattttac taactaacta atctgattta aattttcagg ctaagaacct
     2041 taaaatgccg ggagtctact atgttgacta cagactggaa cggatcaagg aggccaacaa
     2101 cgagacctac gtcgagcagc acgaggttgc agttgccaga tactgcgacc tcccgagcaa
     2161 actgggtcac aagcttaatc ctaggatacc ggtgggtgaa gacccaccaa agccatctgc
     2221 caagggagcc aagaaggccg ccaagaccgt tacgaagcca aaggacggaa agaagagacg
     2281 tcatgcccgt aaggaatcat actccgtcta catctaccgt gtcctcaagc aagttcatcc
     2341 agacactgga gtttcctcca aagccatgtc tatcatgaac tcttttgtca acgatgtctt
     2401 cgagcgtatt gctgctgaag catcccgtct tgctcactac aacaagcgtt ccacaatctc
     2461 atcccgcgaa attcagaccg ctgtccgtct gatccttcca ggagagcttg ccaagcacgc
     2521 cgtgtctgag ggaaccaagg ccgttaccaa gtacacttcc agcaagtagg aattccaact
     2581 gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc
     2641 atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat
     2701 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     2761 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     2821 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     2881 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     2941 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     3001 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     3061 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     3121 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     3181 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     3241 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     3301 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     3361 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     3421 tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     3481 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3541 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     3601 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3661 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
     3721 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3781 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3841 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3901 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3961 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4021 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4081 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4141 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4201 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4261 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4321 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4381 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4441 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4501 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4561 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4621 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4681 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4741 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4801 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5041 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5641 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5701 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5761 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5821 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5881 agcggataac aatt
//
