LOCUS       sequence-441675-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  trp-4(intron 1) fluorescent neural reporter driving nuclear mCyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY141
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3200..3220
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3242..3262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5432..5454)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5554..5573
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5651..5669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5737..5841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5842..5910,5911..6702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6060..6079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6873..7461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7362..7381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7615..7632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7749..7770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7785..7790,7791..7808,7809..7815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7823..7839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7828..7841,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aggactcagg
      181 tttgcctaag tttgatatga aaataataaa aaaacaattt tttgtttctt gaaaaaaaaa
      241 ttttttcgag caaaattttt tttccaaaaa aaaatcttag acactactag aaagttaaaa
      301 gtgttgaaaa cgggtaaagc atatatggag aattcagaaa atcttggttt aacccgttaa
      361 aaaccattag aaagtggcaa aaatggccaa tttatggcaa aaattcacaa ttttgaaact
      421 cctctaaaat gggcaatttt gcagttagag gactaaaatt tgatctgtaa acacttaaaa
      481 atttgccctt tttcagatta tctagagttt tgaatattca aaatcaattt ttaatccaaa
      541 ttcctggggt tttcgactag tttttgggga ggtttgttcg actgtaccac ttccccgtag
      601 aaattttaga aacacaaagt tctatggtat tttgcactca caaggagtga atattcaaaa
      661 ttttttaatt actaatatca atcatgatca ctgaaaatag tttttggtga attttcagag
      721 acatcttggg tttggttttt gagaaatttt taccgaaaag tttttaccca aaattttcga
      781 ttttgcactg aaatttatga gattctttgt gtttttcagg ggtgtactaa gcttgtattt
      841 tattactgaa attatagtta agagcatgtt tagtgagttt cagatggttc tgaagaaaat
      901 tcaaattttt tttcgaaaat aaaatttgaa atttctggtc caaaattttt tatccaaaaa
      961 ttttgctaaa acattgtcct tttttagaat ttgatggttt tatacatatt cgaaaaaaac
     1021 attttttttt ggcccaaaac aaaaaaggac agtcattaga tggctgaaaa atgaaaatgg
     1081 gcaaaaataa aagttgtcta aatttgttga aaacgggtaa ttcatatatg cagaattcag
     1141 aaaaactagg tttaacccgt caaaacctac gaaaaagtgg caaaaatagc caatttatag
     1201 caaaaattcc caattttgaa actcctctaa actattgtcc ataagaaaaa aaacatttta
     1261 caaaattttt gttaaaagtc gcttactttg ttaattaaac tattgctcat aagaaattac
     1321 acataaaaat gtacattgtt ctcttaaact cctaaaaaat cagctgtact tttcctaatt
     1381 ttcagtggaa aatcaaaatt tattgcagaa cttcgatcga atttttaggc gaaatttttg
     1441 tcattttttt ttcaaaaata ttctcaaaat gttgtcttat acttatttag tgagtttgaa
     1501 gaattccgaa aaaaaatttt ttttttggaa atttggaatt tttggccaaa atgttttttc
     1561 ccatcaaatt tgctgaaact caacaattgt gttaatcaaa ctattgtcca taagaaaata
     1621 cacataaaaa tgttcattgc tcccttaaat tccaaaaaaa ataaccgtaa ttttcgaatt
     1681 tttagtggaa attcaaaatt tacagcaaaa accctaaatt tctagcttaa aaatgatgta
     1741 gatttttttt ataaagttat caaaaacatg cagaaaatgt ccttttccag aaaacatatt
     1801 taaattattt gagggtacca gggctccaaa gttgtgtcgg ttttcgtgag cagtactcat
     1861 ttttgctcca aagcttgaaa tcttctcttt tttttagtgt caaattcaaa ttttctgcac
     1921 ttgcaccaaa accgattact aatccccaaa aaacacaata cctactcgct tccagaaaaa
     1981 agtgctcaat ttcctttttt ccagtatctt tgggttcata agacctccca aaagagtagt
     2041 ataagtacac atgaccacac caaacatcgt atctcttaat atacacattt tttgggtatt
     2101 ggcaacacat acacaaaaaa aaagaaaaaa ggtccggaaa tctcaggccg tgtatttctt
     2161 tgaaaaacgg atacgagagc aacctggggg gacagaggta catgaataat tgcttcaaga
     2221 catagctaca cacaaagaat gagaagaaga gaaagtagta tattacttta aatggaacat
     2281 tagagaggat atttggaaat tgttaggggt cgggaatcca gaattgcagg aagttaggtc
     2341 acattacaaa gtacactaat tatttctcat gaaattaaaa atattaaatt tggaatttcc
     2401 tttgatacag caacccacac aatcagtaat aaaaattaaa aattaaaaaa aaaaattttg
     2461 tcgaaaaaaa attttttgat cattttttga aacaacttcg aaagcttatc tctcagttgt
     2521 ttgtggtgtt ttcaaaaagt tgtcaactgg taaaatatgt atttttacat ttactataac
     2581 tttgtagtta aaatcttttt gatatctttg attttaaccg agatatcatg cgccaaagta
     2641 agcataaaaa acatagtgca tcgactggta atacaccact taactttata ggctcatatc
     2701 tcaattgccg ttggttttat aagaaagctt tcaactgaca aaacatttat cattaaattt
     2761 ttgttaacat tgtagttaaa aactttttga tatctttaat ttaaaccaag ataccatttg
     2821 ccaaagagaa gatatcaaac atggtgcatc gaccggaagc acactactta actttatcgg
     2881 ttcatatctc agttttcaat tgttttatca ataaattggc aattaacaaa atatgtaaat
     2941 ttaaattgtc tacaaacttg taatttacgg taaatcgata tcttcaatac taaccgagac
     3001 ataagacaat ttactgaaaa ctaaaaaaaa tgatagtacc tacgattttt ggatttctcc
     3061 tgaaattcca atttccaatt tccagcccgg gattggccaa aggacccaaa ggtatgtttc
     3121 gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga gggtaccgag
     3181 ctcagaaaaa atgaccgctc caaagaagaa gcgcaaggtt ccagtcgaga agatgactgc
     3241 cccaaaaaag aaacgtaagg tcccgcgggt aagtttaaac agttcggtac taactaacca
     3301 tacatattta aattttcagc gcgctgtgtc aaaaggagaa gagcttatta aggagaatat
     3361 gcgttccaag ctctacctcg agggatccgt caacggacac caattcaagt gcacccacga
     3421 gggagaggga aagccatacg agggaaagca aaccaaccgt atcaaggtcg tcgagggagg
     3481 accactccca ttcgccttcg acatcctcgc cacccacttc atgtacggat ccaaggtaag
     3541 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtct tcatcaagta
     3601 cccagccgac ctcccagact acttcaagca atccttccca gagggattca cctgggagcg
     3661 tgtcatggtc ttcgaggacg gaggagtcct caccgccacc caagacacct ccctccaaga
     3721 cggagagctc atctacaacg tcaaggtccg tggagtcaac ttcccagcca acggaccagt
     3781 catgcaaaag aagaccctcg gatgggagcc atccaccgag accatgtacc cagccgacgg
     3841 aggactcgag ggacgttgcg acaaggtaag tttaaacatg attttactaa ctaactaatc
     3901 tgatttaaat tttcaggccc tcaagctcgt cggaggagga cacctccacg tcaacttcaa
     3961 gaccacctac aagtccaaga agccagtcaa gatgccagga gtccactacg tcgaccgtcg
     4021 tctcgagcgt atcaaggagg ccgacaacga gacctacgtc gagcaatacg agcacgccgt
     4081 cgcccgttac tccaacctcg gaggaggaat ggacgagctc tacaagccta ggataccggt
     4141 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     4201 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     4261 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     4321 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     4381 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     4441 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     4501 cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     4561 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     4621 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     4681 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     4741 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     4801 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     4861 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     4921 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     4981 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     5041 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     5101 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     5161 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     5221 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     5281 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     5341 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     5401 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5461 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5521 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5581 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5641 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5701 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5821 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5881 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5941 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6001 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6061 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6121 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6181 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6241 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6301 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6361 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6421 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6481 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6541 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6601 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6661 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6721 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6781 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6841 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6901 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6961 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7021 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7081 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7141 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7201 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7261 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7321 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7381 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7441 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7501 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7561 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7621 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7681 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7741 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7801 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
