LOCUS       sequence-441678-        8106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-42 fluorescent neural reporter driving nuclear mCyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY145
FEATURES             Location/Qualifiers
     source          1..8106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3465..3485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3507..3527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5697..5719)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5819..5838
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5916..5934)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6002..6106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6107..6175,6176..6967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6325..6344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7138..7726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7627..7646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7880..7897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8014..8035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8050..8055,8056..8073,8074..8080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8088..8104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8093..8106,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ttaggtcctg
      181 tcgatgccat ttttgtggaa cgagaatgaa ctcaaagcac aagtctaatt aagaatgtat
      241 gaaggaaagc agtcgcaaaa aaccaagaat tgacaaaact atggaggact aacgagatga
      301 aagtcttcga tgaacggagg gagttgtcgt cggaaaaaag aaagagtagg agcaaaaggg
      361 aaaagggatg acacgatgag atgacagcaa aatctgcaaa agtgaggaga cagaaagctt
      421 gtaggagcaa gaggaagctc caattttttc cgagggaaat agaaatcaaa tcaacgaatt
      481 gtgagggagt catgatgatg atgatgatta tgatggtttt gggaagaaac atgggagcat
      541 cttcttctgg tttttggttt ccagaatatg atcttgaggg cgagtgcatt aaactttcgg
      601 ggtccaacag tgaaaattca tattaccgtt gttaatatta gtcttgaact ccaactgctc
      661 cagcccatat attgattaac tttggtttga tccaagtgat caactttgaa aattgataaa
      721 attaaaagcc aggatcaaag tcatcctttt tatagacctg gacgcaaccg taccataatc
      781 ttccaattgg gtaaatggaa tgaaacagtg aataaatctg ctaaaattct acaaagaaat
      841 gtctgctttc aaaaataaat taatggatcc acttactcac aaatgacttc tacagtctaa
      901 ttttttttaa gtttgaaaat ttaatttcaa actatactgt tgtacttctc attgctagat
      961 aatttttccg ctttttgcca tttgataata atattttgat gaaaaaaaca tacgctatct
     1021 tgaatatggt tactttcaaa aacaactggc cgtatgtgtt ctactcaaaa gtttctctaa
     1081 atttaataga tcatttcggt aattacaatt tgttttatga ggttataatg attagtatca
     1141 ttcaataaat atacttgagc atgatgagag gagaagtgcc aaaggcttgg gcacaatccg
     1201 tcgttatacc actagggaaa actactaaac ctaaagactc aaaagacttt cgtcctgtta
     1261 gcctcacgtc aaatatctgt cgagtatatg agcgatgctt actgaaaaaa atagaaccgt
     1321 atcttagaga aatcaatttt tggaatgaga accagcatgg gttcagacct acaaaatcaa
     1381 cgatctctaa tctgatgaat agtttgaatg actggacaag caacctagat ctgggagccc
     1441 aaattgacgt catatttaga ttacagcaag gcatttgaca gaattcccca cgacattctc
     1501 ctcaataaac tgatacgcct gggactgaat aaacatatca tagtctggat caatagtttt
     1561 ctaatagaaa ggacatttag agttaaaata ggaaatacac tatctactgt aaaaaacgct
     1621 aaatgtgggg ttccgcaagg cgctgtactc tcaccagttc tttttggaat ttacgttaat
     1681 gagatcccaa caatactgcc cgcaaatgtt aaagtgcagc agtttgcaga tgacaccaag
     1741 ctgtatgtac acacgccaaa aggcgaccag attaatagtc tgcaagaagc aatagacaga
     1801 gtagcaaatt ggtccgtcag taacaaattg gctcttaatg aacaaaaaaa ccgtccatct
     1861 cacgataggg aaaaaacgcc aaaatttcac ttataagata aacggccagg atatcaaaaa
     1921 agagtcaacg acacgtgact taggttttat gatctctcat aatctggatt ttagtgaaca
     1981 ctggaaaaag gctattaatt cctcgaaatt tcaaatgtca caaattttta accattataa
     2041 cagtaacaaa ccaaagctga tgatcctatt atataaaaca tttattgtgc ttttgctaga
     2101 gtacggaacc gttataagta gcccaccaaa gcagtcagat ataggagcca tcgaggcagt
     2161 tcaaaattcg ttcacaaggc gtctatatag taggactgta ggcaaatacg tctcaataag
     2221 tgatccggaa tataaaacct cgacagaaag aaatgaatta tttgggctca caccgctgca
     2281 aggacgacgt ctgacaaccg ataaaaaaat gattttgaaa atgttaaacg gtaaaataga
     2341 tctgaaagta cctgaatttt ttaccttctc ggacaacact agaacgcgtt gtaaaagaaa
     2401 gttctcttgg agcagatgta aatttaaaat taggaaacat ttcttcgtta acagaacatt
     2461 aagccaaccg tcattcctca aatttttatc taatttaaac aaggataact aagttaggaa
     2521 acgaacgaca tctactcttg atcaataaca ttcattcatt actatcctat gatatctcct
     2581 gtttttttat cctactcctt ctcatgctct ttttctacgc ctcaggttct ttcactttgc
     2641 ccataatctc ccagtccacc ctccgacatg aatctttgta tgtatcatct tttttctttt
     2701 cacgcttctt caagaacgta ataaattatt attattgtta ttattaaatg actacaaaca
     2761 ttctgattac atttcccttt ttttaaacat atcgcagcat ttctacgttt aaataattca
     2821 attaattatt tttagcgtaa ctgagtattt tagtttaacc acaaattaaa tcccctttca
     2881 aatcgaaccc acaatttttc caacccaatt tggatttagt ccgtgtgttc gtgtggggtt
     2941 cgccagttga actgcttctc taattgcgat tatcgccacc cggcttcaga gtccgtccaa
     3001 actcgattat agctttcgct ctccaccgat cactgaattc gttgccgtta agtgtagaag
     3061 ctcagttgaa gaataagaag acggaaagaa gaagctgtca aataatctaa tttacttgac
     3121 tctctcaaac caatccaaat caaaaagaag gcgtggctac ctagctcttc tggcggaaag
     3181 atgggtacgg tggttggggc cggtggtgaa ggaggaggag cagccggagg cggacggcga
     3241 ccagtcagct gcccctctct tcagaagcaa ccctgtccgc ctccacgacg acgtcgacaa
     3301 cgacttcggt cacccctcac tttccacatt tctccgcttt cactcacaca cccgggattg
     3361 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     3421 ggacccttgc ttggagggta ccgagctcag aaaaaatgac cgctccaaag aagaagcgca
     3481 aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg cgggtaagtt
     3541 taaacagttc ggtactaact aaccatacat atttaaattt tcagcgcgct gtgtcaaaag
     3601 gagaagagct tattaaggag aatatgcgtt ccaagctcta cctcgaggga tccgtcaacg
     3661 gacaccaatt caagtgcacc cacgagggag agggaaagcc atacgaggga aagcaaacca
     3721 accgtatcaa ggtcgtcgag ggaggaccac tcccattcgc cttcgacatc ctcgccaccc
     3781 acttcatgta cggatccaag gtaagtttaa acatatatat actaactaac cctgattatt
     3841 taaattttca ggtcttcatc aagtacccag ccgacctccc agactacttc aagcaatcct
     3901 tcccagaggg attcacctgg gagcgtgtca tggtcttcga ggacggagga gtcctcaccg
     3961 ccacccaaga cacctccctc caagacggag agctcatcta caacgtcaag gtccgtggag
     4021 tcaacttccc agccaacgga ccagtcatgc aaaagaagac cctcggatgg gagccatcca
     4081 ccgagaccat gtacccagcc gacggaggac tcgagggacg ttgcgacaag gtaagtttaa
     4141 acatgatttt actaactaac taatctgatt taaattttca ggccctcaag ctcgtcggag
     4201 gaggacacct ccacgtcaac ttcaagacca cctacaagtc caagaagcca gtcaagatgc
     4261 caggagtcca ctacgtcgac cgtcgtctcg agcgtatcaa ggaggccgac aacgagacct
     4321 acgtcgagca atacgagcac gccgtcgccc gttactccaa cctcggagga ggaatggacg
     4381 agctctacaa gcctaggata ccggtgggtg aagacccacc aaagccatct gccaagggag
     4441 ccaagaaggc cgccaagacc gttacgaagc caaaggacgg aaagaagaga cgtcatgccc
     4501 gtaaggaatc atactccgtc tacatctacc gtgtcctcaa gcaagttcat ccagacactg
     4561 gagtttcctc caaagccatg tctatcatga actcttttgt caacgatgtc ttcgagcgta
     4621 ttgctgctga agcatcccgt cttgctcact acaacaagcg ttccacaatc tcatcccgcg
     4681 aaattcagac cgctgtccgt ctgatccttc caggagagct tgccaagcac gccgtgtctg
     4741 agggaaccaa ggccgttacc aagtacactt ccagcaagta ggaattccaa ctgagcgccg
     4801 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     4861 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     4921 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     4981 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     5041 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     5101 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     5161 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     5221 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     5281 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     5341 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     5401 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     5461 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     5521 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     5581 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     5641 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5701 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5761 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5821 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5881 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     5941 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8101 acaatt
//
