LOCUS       sequence-441681-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pdfr-1 (3-2K) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY115
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1489..1509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1534..1554
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1576..1596
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3405..3427)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3527..3546
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3624..3642)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3710..3814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3815..3883,3884..4675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4033..4052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4846..5434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5335..5354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5722..5743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5758..5763,5764..5781,5782..5788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5796..5812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5801..5814,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cactactttc
      181 agcgaccttt acttgctcaa tttgaacttt ttaaaaatct agcttgaaac atgattgccc
      241 taaaccgatc aagtgcctga catcaaaaga aatagaattt tcagtagttt tttgttgttt
      301 ctttttaaat tttattttat ggactgtcat tgtaacacca ctaatataaa aaatgttctc
      361 gtggactgtc gaagcaattt ttactaatta tcattgacga caaccacaat ttatttttga
      421 tcagcccatc tcatattacc caaaaatata ttttctcaaa tgaaaacttg aatttctata
      481 aaatggcaca aaaaatatgt tttcccccac tttccatgac tggcttctac ttgatatgtt
      541 catgtcaaac tcagtggaca ccaccatcac aaaaaagatt ttcagagaaa aaaaacgaat
      601 aagagaaagt cacatcttct caaaatttta tctctggaaa ttttgttctc ttcttttaca
      661 cttttttccc ctactttctc gaccctcgtc attcttagaa ggacgggctc attcgcgcct
      721 cattgcattt taaccttgat ttctccccct cctaaacctt tttctctgat ctttttcttt
      781 gcttttccat aattcattaa aacgccacaa agttttatgt tccaagttcg ttttaaatga
      841 tgcttggttt tgccgtcggg tatttcccaa aaaactaatt acttcggacg ggtctagacg
      901 tttttaagaa aaatagggtt aaaaacaggc caagttttgg ccatttgaaa aaagaaatcg
      961 ctcgaggcaa aattttaaaa attcaaacat tatctaccaa caatcaagtt acgtaggtta
     1021 cagttgaaat ttatggaaat atagtaaact agctctaaga tacattttcc ataggataca
     1081 tttttagaat aggctttcca aaaaatcaag tcgtctaact aaaagtttaa gaatttttaa
     1141 catctataaa atattaacag cctcatatga gtatttttta taatatttcc acgattgctt
     1201 tttgttgtat aactaagtgt ttttttttct tatatcttgt cttttttctc tttcaccaaa
     1261 tctctatgct tctgcttagg gcacgccttc ccattttctt ttctttattt tcatttgtct
     1321 cctcccaccc tccaaatgtc ttgaaactga cccgggattg gccaaaggac ccaaaggtat
     1381 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg
     1441 ttttcccagt cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaaa
     1501 cgcaaagtac cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa agtaccggtc
     1561 gccgagaaga cagccccaaa aaagaagcgt aaagttccac gcgtcatggg agtaagttta
     1621 aacagttcgg tactaactaa ccatacatat ttaaattttc agcgcgctgt ctccaaggga
     1681 gaggagctca tcaaggagaa catgcacacc aagctctaca tggagggaac cgtcaacaac
     1741 caccacttca agtgcaccca cgaaggggag ggcaagccct acgagggcac ccagaccaac
     1801 aggattaagg tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc
     1861 tttatgtacg ggagcaagac cttcatcaac cacacccaag gaatcccaga cttcttcaag
     1921 caatccttcg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     1981 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gcttaccgtt
     2041 acccaggaca ccagcctcca ggacggctgc ttgatctaca acgtcaagct cagaggggtg
     2101 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc
     2161 gagaccctgt accccgctga cggcggcctg gaaggcagat gcgacatggc cctgaagctc
     2221 gtgggcgggg gccacctgca ctgcaacctg aagaccacat acagatccaa gaaacccgct
     2281 aagaacctca agatgccagg agtaagttta aacatatata tactaactaa ccctgattat
     2341 ttaaattttc aggtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat
     2401 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     2461 ctggggcaca aacttaatgg catggacgag ctgtacaagt aagcgccggt cgctaccatt
     2521 accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg
     2581 agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac
     2641 ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     2701 ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag
     2761 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     2821 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     2881 ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa
     2941 aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat
     3001 ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg
     3061 agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc
     3121 tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa
     3181 tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc
     3241 agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc
     3301 cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg
     3361 tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3421 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3481 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3541 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     3601 taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3661 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3781 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3841 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3901 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3961 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4021 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4081 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4141 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4201 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4261 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4321 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4501 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4561 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4621 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4681 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5581 cgagcgcagc gagtcagtga gcgatgaagc ggaagagcgc ccaatacgca aaccgcctct
     5641 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     5701 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     5761 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
