LOCUS       sequence-441682-        6986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-86 fluorescent neural reporter driving nuclear mCyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY147
FEATURES             Location/Qualifiers
     source          1..6986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2345..2365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2387..2407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4577..4599)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4699..4718
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4796..4814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4882..4986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4987..5055,5056..5847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5205..5224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6018..6606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6507..6526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6760..6777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6894..6915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6930..6935,6936..6953,6954..6960)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6968..6984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6973..6986,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tgtcatgtcg
      181 tgtgcaatgt cagttgctca ccaactgaaa tacctcagcc ggaatgtacc catctggtgt
      241 tgtgcgcgtg gctcctgaca ctatgagctc attcactcat tttcccgcac agtattgtag
      301 tgttcatctg aagcgtgaga gattatagag ttgaatagaa gtggaataga gagtgagaca
      361 cgtatgaaaa ggtgcagctt gcagtacttg agtgagaaca atagaaaggg aaagaggaag
      421 aaagatacta attgactaaa gtgtcaaaag agcacacatg gatttaatat tgaaatgaat
      481 gaacaatatg agcaaaaaaa acatatctgc taaaacttat gattaaattt aaaatttgaa
      541 tttgaaaaca agttcattca aaatttctga actttaacga gtcagtatct ataattttta
      601 gaagtagaat caccttgcaa tcagttatca agattttaac tgttatgggg ttattcaagt
      661 atataaaaac atgtatttaa atacatgttt ttatatactt gaatacagtt gtgaaataat
      721 tcttctacac tttttaattt tccgacacta cttgaataac cccattacgg cagttaaaat
      781 ctgatttttt tctaaataga aaagctaccc cgaataaata caaaataaac taccccatta
      841 ttacacaaga aataagaaaa cacgggaccc atctcattct caactttttt gaccaatcat
      901 gaataaaata cattctcaat cccttctatt tttcattatt tttcttaaaa atccgacaat
      961 ttctgtctcc gatcgtagtc tcttttcttt cttcttcttc ccacacctta ttattaatgt
     1021 atgactcttc tgctcgggca tcggcggggc gacaacgacg aagctcatgt gtctcttcac
     1081 atccatcctt ttcctttttc gtttaacctc cttcgtttgt ttgatacggt agtatgggaa
     1141 gaaaaaagaa aaagaaaaag aaaaagatgg aataaaaggg aaaaagaaca aaaacctcga
     1201 tgtgggttac tggaaaaaaa aaactgaata gaataaaatg tcgattggaa ggattactgt
     1261 aggtaaaact tgtgacgtca ttacaatcta acgtctaacg tcattacatg aacgatgttt
     1321 tttgatgata aaaaaagcga acaatcgtag cctgcctcgt gcctgcttgc ctacttgtct
     1381 tcctacttat gtttcagatg aagctatgcg acacggtgga gctggccgct ccagatagaa
     1441 gctcataaac gattttaaca acaggggagt ctcaagcttt ctgatttcaa accgtattga
     1501 aaacagctcg aaaaataact gccaagaata ggggaaaatt cagcgaattt ttgtaaagtc
     1561 aatttcaaat tttacaggaa cttcaaggtt gaggatccaa gttatcaaca tgatgtttta
     1621 agttttcaat gttttcttaa gaataacagt acgatttttt aaagaatcac tgtagtttga
     1681 catttacgaa actgaaaatt tacgaaaaag aaaactttaa attttaagtt gccagcttga
     1741 aaagtaatcg aagttggtta tttccaaaat ggaccacttc agttttgatg gtaaatatag
     1801 tagcagaatt cagaattcca gctgtggaaa atgcattaaa attatttttt ttttttctaa
     1861 aacgaaaagt tgaaaacgaa tgaaaaagtg aaattgaatg aatggagaag gcacatcgat
     1921 tccgtttgcc tttttgctcc ttctttcccg tccctctcct tgtgtcggtt ttaatatttc
     1981 atcactccgc tccattttta attcaactct tttttccttc ccccctcttc aacccccttc
     2041 tccgcccttc caaaatcagt agaggttctg aaaatggggc gtgcactagc cacagtaccc
     2101 tccaacggac cagaaaaatc atccaatggg agctgcctaa aaggggtgga gaccaagaca
     2161 taggggaccc ccgacccttc cgtatacatc tgtctcctcc cagcttcaag gtccccctct
     2221 tttccacgga cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     2281 aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag aaaaaatgac
     2341 cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa aaaagaaacg
     2401 taaggtcccg cgggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     2461 tcagcgcgct gtgtcaaaag gagaagagct tattaaggag aatatgcgtt ccaagctcta
     2521 cctcgaggga tccgtcaacg gacaccaatt caagtgcacc cacgagggag agggaaagcc
     2581 atacgaggga aagcaaacca accgtatcaa ggtcgtcgag ggaggaccac tcccattcgc
     2641 cttcgacatc ctcgccaccc acttcatgta cggatccaag gtaagtttaa acatatatat
     2701 actaactaac cctgattatt taaattttca ggtcttcatc aagtacccag ccgacctccc
     2761 agactacttc aagcaatcct tcccagaggg attcacctgg gagcgtgtca tggtcttcga
     2821 ggacggagga gtcctcaccg ccacccaaga cacctccctc caagacggag agctcatcta
     2881 caacgtcaag gtccgtggag tcaacttccc agccaacgga ccagtcatgc aaaagaagac
     2941 cctcggatgg gagccatcca ccgagaccat gtacccagcc gacggaggac tcgagggacg
     3001 ttgcgacaag gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     3061 ggccctcaag ctcgtcggag gaggacacct ccacgtcaac ttcaagacca cctacaagtc
     3121 caagaagcca gtcaagatgc caggagtcca ctacgtcgac cgtcgtctcg agcgtatcaa
     3181 ggaggccgac aacgagacct acgtcgagca atacgagcac gccgtcgccc gttactccaa
     3241 cctcggagga ggaatggacg agctctacaa gcctaggata ccggtgggtg aagacccacc
     3301 aaagccatct gccaagggag ccaagaaggc cgccaagacc gttacgaagc caaaggacgg
     3361 aaagaagaga cgtcatgccc gtaaggaatc atactccgtc tacatctacc gtgtcctcaa
     3421 gcaagttcat ccagacactg gagtttcctc caaagccatg tctatcatga actcttttgt
     3481 caacgatgtc ttcgagcgta ttgctgctga agcatcccgt cttgctcact acaacaagcg
     3541 ttccacaatc tcatcccgcg aaattcagac cgctgtccgt ctgatccttc caggagagct
     3601 tgccaagcac gccgtgtctg agggaaccaa ggccgttacc aagtacactt ccagcaagta
     3661 ggaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     3721 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     3781 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     3841 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     3901 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     3961 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     4021 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     4081 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     4141 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     4201 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     4261 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     4321 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     4381 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     4441 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     4501 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     4561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4681 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4741 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     4801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5221 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5521 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6781 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6841 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6901 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6961 tgtggaattg tgagcggata acaatt
//
