LOCUS       sequence-441683-        7872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  trp-4 fluorescent neural reporter driving nuclear mCyOFP1 expression
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY140
FEATURES             Location/Qualifiers
     source          1..7872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3231..3251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3273..3293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5463..5485)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5585..5604
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5682..5700)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5768..5872
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5873..5941,5942..6733)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6091..6110)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6904..7492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7393..7412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7646..7663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7780..7801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7816..7821,7822..7839,7840..7846)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7859..7872,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt taagtcccat
      181 gcgttgcttt ttggtggcgc tacattcata caaatgacca aaaatgcttg caaaaatgac
      241 tttttaaact ttgaaggacg ctaacttccg caacgtgcac gataccaaaa agtaataaat
      301 tacaacatta atgagcacat caattactac attttattag tttaccattt tttcctatct
      361 ttactcgctg ctaagttaca gtaagttgaa acaaactaag tgcaagtttc aaaaaacacc
      421 cttttttcca gatttttgcc tgtatctccg aaaccagttg tttaacaaaa attttgtcaa
      481 caaacgaaac atttaccttt taattttacg cattttcgta gttgaaaatt ttgtcatcat
      541 ataagtgttg gcaaagatat gagacaatta agcgagctcg tccaagtttg tgaaaatttt
      601 cgaaaatcat cgtgaaaaat ttcgttaaaa aaattttcag agaaaaaggc ctaaacattt
      661 ttgaatatga ataagtaact tttagggtca aaaatcgtta ttagaaattc ctgccaaaat
      721 tcctgccaga acttcttgcc ctttttctat aggccctaaa aactgtcatt tcgcacctaa
      781 aactccctcg tctaatgacc gggttctcca acacattacc ttctcacttt cgccgtaaaa
      841 atgctaaatg cgaactcttt tacttattcc tcatttctca tttctcacat tcttcttact
      901 ttttgttctt tttttgtgac ttccagatgt tcaacttctc cctacctcca gttctataac
      961 attttcatag aaaaagaaaa agtatatgta ttttttgcta ttcccccctc gccttgctcc
     1021 agagagcttc tgctgaccgg aaatggtcat tttagggtgt gcacctggtc tgtctctctc
     1081 ttctacacaa cccctcctca cctctattac ttttgagagg gtccgtgctc tgccgacatc
     1141 acattcacca ccaccactaa agttggaaaa aatcactttt ttttggagat ttattattct
     1201 ttttatagaa gcctttttgt gggcggagcc tacttagggg cggagactaa aattcaaatt
     1261 ttcctgataa aactgtttgg cgggaattca attttctgag aatttttttt tttcaaaaaa
     1321 ctttagtgtt tcatactttt ttgtgggcgg agcctaacaa gggaagtcga aaaactgaac
     1381 cccggacttt gacatgccat agtaattttt cgataaaaga gtgaaaataa tgatctctcc
     1441 aaaaaattta gctgccccgg tccaggttca gcaaagttat gacgttttga aagtggctag
     1501 acaacctttt taaaaaaatt tttaaaattt caaaaaaaaa actttttttc tgaaaagtgg
     1561 aaaaaatatt tgcaaataat tgaactcagt gaaaaacata tgccatgaaa gttttagctc
     1621 ataactctca agaaaaccca taaattaacc tacaagaaaa agtaattttt ggaattttgc
     1681 tcaagatatg tagatcaagc aatgtggttt tttgtccgtg ggaagaaaaa atgtgcatga
     1741 tatatgtttt ttacattttt ggataagtta caatagtttt tatcaatttt tggaaaaaaa
     1801 attttttttg aaaatttgag ttttttttgc tagggttttg ctagggtcaa ggaatttttt
     1861 tcggcacttt caaaacgtca taactttgct aaacctggac cggggcagct aaattttttg
     1921 gagggatcat tattttcact cttttatcaa aaaaatacta tgtcatgcca aagtccgggg
     1981 ttcagttttt cgacttccct tgtaaaattc aaatttttca gtaaaacatt tttgcggaaa
     2041 ttcaaatttt ctgagaaatt gtttaaaaga taactttagt gttttatgcc tttttgtggg
     2101 cggatcccaa ttaggtgcgg agcctaacat ccaaattttt cagtaaaata tttttgcgaa
     2161 aattcgaatt ttctgaaaaa agttttctaa atgataactt tagtttttta tgtttttttt
     2221 ttgtgggcgg agcctaaata ggagcggagc caaaaattct aatttttcag taaaataatt
     2281 ttggcgggaa attcgaattt tctgagaaca tttttttaaa aagattactt tagtgtttta
     2341 tgcctttttg tgggcggagc ctaataagtc gccggaaatt aaaattttct caaaaaacta
     2401 gttaaaatat ataattttaa gaatattctt gctttaagtt tttttttgcc gacctagaaa
     2461 aatgtgtctg tttgtagttt gtagtctttg acgtcacaag ttgtgtgacg tcacacaccc
     2521 cacgcccctt ttgtggggcg gagtctgaat agttgatacc ccgggaaatt aaaatgttct
     2581 cttataattt cttggcggga attcaaaatt ttgtttctaa aaatttttgc cgggaaactg
     2641 gaattttatg agaaaaattt tttggcggga agttcaaatt gtctcagaaa cattttggcg
     2701 gatattcaaa tttaattttt ttttaaattt tgcctggaaa tgcaacagca attatttttc
     2761 aaattttggg gagtttgaat tttcgtatat tttgcaaaat tttctcacag taattaagaa
     2821 ttctttgttt taaaattaca tccatctcct agactacagt aatcctacag tatcctcaca
     2881 gtagatcctt tttaaattta tacctatttc ctaaaaaaaa tcaatgtttc catagaaacc
     2941 attaaaaact tttttactat agtatttcgc aattcttttt ttttcatttt tttaaatctt
     3001 ttcccccagc ctcttcaaaa tctccaattt tcagcaaaat tcctcggctc ctcaaaataa
     3061 atcatcatat cttaataata ctactactat taatagcatt atacattacc ccggttcccg
     3121 ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt
     3181 tcaggaggac ccttgcttgg agggtaccga gctcagaaaa aatgaccgct ccaaagaaga
     3241 agcgcaaggt tccagtcgag aagatgactg ccccaaaaaa gaaacgtaag gtcccgcggg
     3301 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag cgcgctgtgt
     3361 caaaaggaga agagcttatt aaggagaata tgcgttccaa gctctacctc gagggatccg
     3421 tcaacggaca ccaattcaag tgcacccacg agggagaggg aaagccatac gagggaaagc
     3481 aaaccaaccg tatcaaggtc gtcgagggag gaccactccc attcgccttc gacatcctcg
     3541 ccacccactt catgtacgga tccaaggtaa gtttaaacat atatatacta actaaccctg
     3601 attatttaaa ttttcaggtc ttcatcaagt acccagccga cctcccagac tacttcaagc
     3661 aatccttccc agagggattc acctgggagc gtgtcatggt cttcgaggac ggaggagtcc
     3721 tcaccgccac ccaagacacc tccctccaag acggagagct catctacaac gtcaaggtcc
     3781 gtggagtcaa cttcccagcc aacggaccag tcatgcaaaa gaagaccctc ggatgggagc
     3841 catccaccga gaccatgtac ccagccgacg gaggactcga gggacgttgc gacaaggtaa
     3901 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcaggcc ctcaagctcg
     3961 tcggaggagg acacctccac gtcaacttca agaccaccta caagtccaag aagccagtca
     4021 agatgccagg agtccactac gtcgaccgtc gtctcgagcg tatcaaggag gccgacaacg
     4081 agacctacgt cgagcaatac gagcacgccg tcgcccgtta ctccaacctc ggaggaggaa
     4141 tggacgagct ctacaagcct aggataccgg tgggtgaaga cccaccaaag ccatctgcca
     4201 agggagccaa gaaggccgcc aagaccgtta cgaagccaaa ggacggaaag aagagacgtc
     4261 atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt cctcaagcaa gttcatccag
     4321 acactggagt ttcctccaaa gccatgtcta tcatgaactc ttttgtcaac gatgtcttcg
     4381 agcgtattgc tgctgaagca tcccgtcttg ctcactacaa caagcgttcc acaatctcat
     4441 cccgcgaaat tcagaccgct gtccgtctga tccttccagg agagcttgcc aagcacgccg
     4501 tgtctgaggg aaccaaggcc gttaccaagt acacttccag caagtaggaa ttccaactga
     4561 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     4621 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     4681 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     4741 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     4801 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     4861 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     4921 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     4981 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     5041 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     5101 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     5161 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     5221 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     5281 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     5341 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     5401 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5461 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5521 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     5581 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5641 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     5701 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5761 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5821 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5881 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5941 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6001 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6061 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7861 cggataacaa tt
//
