LOCUS       sequence-441684-        8022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sax-3 fluorescent neural reporter driving nuclear CyOFP1 expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY116
FEATURES             Location/Qualifiers
     source          1..8022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3381..3401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3423..3443
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5613..5635)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5735..5754
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5832..5850)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5918..6022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6023..6091,6092..6883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6241..6260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7054..7642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7543..7562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7796..7813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7930..7951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7966..7971,7972..7989,7990..7996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8004..8020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8009..8022,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gcgtactaac
      181 aatgggcccc cacccttccg cagctccgta acaaatttgt tctgaagccg tagtacatca
      241 cgctgtcatc tgcttttgtg cccccccccc ccccgtccat gtttcctctt ccgtcgtcag
      301 tagccgttac tcataattct atttgaaggt aaaccaattt gggaggaaga gaagaaggat
      361 gcccgatgga tgctgcttct tcctcttatt cttcttgtgc tccacaaaac tctcttcccc
      421 cccccccccc gttccattct tctattcctc ttctctgttc gaaaacctct tcttcatcaa
      481 ccgcttcaat ttgagtttct ccttttcttt catctatact tcttttcttc catttcgtat
      541 tcatttattg agagagctca cccgcaccct tctcctctcc tgcatccttg tcgggtttcc
      601 gctttcatca tcttcgccgc cttattctgg aaaacagatt cattatgcat atcatctttc
      661 ggagtcagtc agcttttcat ctgctttcac ggatggctgt cgtgaagtcg caaaaataat
      721 aaaatgtgca attgccctga aaaacacgaa aagtcgagaa aaaaaagcca tcggaaaaca
      781 tgtgtgtttc tgttttctgg ttcattttga tacgcaagat gtagtgtatt agtgaataaa
      841 atgttctgtc ttgtttttta aagcattttt cttagtatct tctagaaagg tataaaatcg
      901 tttaaaagtg gcgcatgact aaatatcata cacagttgaa ctttttaaaa cttttttttg
      961 ttgaattttt tcatttttac aaacgtgtgt attcagtttt tgttactttt ttggacgttg
     1021 tcaatcaaaa aaaaaattca aattttaata ttaaaactct gtggggattt gaaaacgcga
     1081 cagtgctaat ttttcatttt aaaaaatgca aaaactcagt atttgccact ttttgacagt
     1141 cagcaaaaac ctttgaaaat ttttgaaata ttttacaaag atatttagtt attttagcac
     1201 cataggagta gttttcacca atctccctac cgcgctactt taacatttgt gccaaaggtg
     1261 cgcggcaaat gcaggctggc aattctacaa aatgagccag tctggcatgt tccccgaagt
     1321 agtttttttt taaataaaaa tttagttaca aaaaatggaa aattaatttt ttatctgaaa
     1381 ataacttgtt ctctatttga aacaattgaa aaagtgctca aaaattgtct gaaagtttga
     1441 aattgccgaa aaatatacgt gcatgtatta aaaacttcca tatcataatt tctcacgcac
     1501 ctcatgtttc tttggattta aaaagagaga ttcaatatca ccgagagagc aggaatattt
     1561 gcataaccca cttttgctca ccgtcgtctg ccactgacca cttgctccgc cggcgaccca
     1621 cttcttggcg gggcaaagga gcgcggaatt gagaaaagat gggaggggga aaaaatcatt
     1681 tggcgcacac tttcgtgccc ctctttgacg tcccccattc caatccacgt ttttcacgcc
     1741 atcgacccgt cgtccggtcg agcaggtgtc cagtggaccc catgacgaat tgcggcggac
     1801 cacaattcta ttctccctgg tagactttcc catcccatca cacttttctt tgcatttttt
     1861 gcttcttttt tttttctttt tggttattgt gccatctttc ttctgaactt tttgcttttc
     1921 ggaaaaagag gtgatcttct catttcaacg aacaaacgca ctttgtaaag tgccataaag
     1981 tttattgtcc agcttctcgc cccgccgaca cctttgcgaa attatggtca attcctgtca
     2041 ctggttctca cttttttact ttccacattt ccccaccgtc tgcttccccg caaaaatcac
     2101 aggctcttcg aatctcccca cctattcttg cgaattttat cggttccact acaagtgtaa
     2161 ctattgctca ttgacaacgt atagacacaa cacgacagat gttgtatgac agggccgggt
     2221 tttccttctt cttcgtcgtc ctcccgtccc ccaatcgttt gcctcgttgg tccatgggca
     2281 taggttcggt ctacaatgga cctaatgaac gggtagtcga atgtgagagg agggcggcca
     2341 cgaattggaa taattactta accagggaag ggggcgacgt atagccccgg ctatcccctc
     2401 atattccatg cggtttcgtt ccgtatcgag ccgcttgcat gacatggaca tccgtcatcg
     2461 ccgtgctctt gacgcgcatt atttaggtta tttatctgac aattatgagc tttctgacga
     2521 ccttattgag ttgagagctt tgatatgttc tcgagtactt tcttgtctag aataaagcgt
     2581 attgattttt cttattcgcc cgcctgttgc cagatagttt atctaatcag tattattcaa
     2641 tcgctggcac aacataaatc gatacttgtc tcactcctct ttgcgcgttt atctccccgc
     2701 gcattgcaca cacaccttga agaggaaatc tgatgtctaa cattctgcgt ctacgccttc
     2761 tagcaattcg tctcaacggt cctcatttta aatgttctgc aaatgcattt gtactaaatt
     2821 gtgatttgtt tctttttggt tgttagagaa acaatgaaag ctttttgtat gtataaaaat
     2881 tttgaaccat ttggtgatgt tgaaaatccg tttttctttt aatttcaagg tattttccta
     2941 attttggcca ttttgctgtg tttgaagttt ttaatttcca tgttcaaatt gtagctatag
     3001 ttattttatt gatactctgg caaaaatcaa atttcccaag taaagtaaaa cgcctatata
     3061 gacttgatga agttctactt agattgaaac ttcaacggat tcttactaca atcttgcaca
     3121 actcctgcgt aaaataaaat tttgcatagc ttttcgggag tcttgtgcag tttgaaacaa
     3181 ctcctgcaca cttcccaatc tgccacgaac ttagtactaa agatttttta tattttttgt
     3241 cagagtaggt ttcctttttc aagttgcccg ggattggcca aaggacccaa aggtatgttt
     3301 cgaatgatac taacataaca tagaacattt tcaggaggac ccttgcttgg agggtaccga
     3361 gctcagaaaa aatgaccgct ccaaagaaga agcgcaaggt tccagtcgag aagatgactg
     3421 ccccaaaaaa gaaacgtaag gtcccgcggg taagtttaaa cagttcggta ctaactaacc
     3481 atacatattt aaattttcag cgcgctgtgt caaaaggaga agagcttatt aaggagaata
     3541 tgcgttccaa gctctacctc gagggatccg tcaacggaca ccaattcaag tgcacccacg
     3601 agggagaggg aaagccatac gagggaaagc aaaccaaccg tatcaaggtc gtcgagggag
     3661 gaccactccc attcgccttc gacatcctcg ccacccactt catgtacgga tccaaggtaa
     3721 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggtc ttcatcaagt
     3781 acccagccga cctcccagac tacttcaagc aatccttccc agagggattc acctgggagc
     3841 gtgtcatggt cttcgaggac ggaggagtcc tcaccgccac ccaagacacc tccctccaag
     3901 acggagagct catctacaac gtcaaggtcc gtggagtcaa cttcccagcc aacggaccag
     3961 tcatgcaaaa gaagaccctc ggatgggagc catccaccga gaccatgtac ccagccgacg
     4021 gaggactcga gggacgttgc gacaaggtaa gtttaaacat gattttacta actaactaat
     4081 ctgatttaaa ttttcaggcc ctcaagctcg tcggaggagg acacctccac gtcaacttca
     4141 agaccaccta caagtccaag aagccagtca agatgccagg agtccactac gtcgaccgtc
     4201 gtctcgagcg tatcaaggag gccgacaacg agacctacgt cgagcaatac gagcacgccg
     4261 tcgcccgtta ctccaacctc ggaggaggaa tggacgagct ctacaagcct aggataccgg
     4321 tgggtgaaga cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta
     4381 cgaagccaaa ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca
     4441 tctaccgtgt cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta
     4501 tcatgaactc ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg
     4561 ctcactacaa caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga
     4621 tccttccagg agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt
     4681 acacttccag caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct
     4741 ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac
     4801 taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa
     4861 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     4921 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     4981 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     5041 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     5101 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     5161 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     5221 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     5281 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     5341 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     5401 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     5461 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     5521 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     5581 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5641 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5701 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5761 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5821 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5881 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5941 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6001 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6061 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6121 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6181 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6241 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6301 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6361 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6421 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6481 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6541 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6601 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6661 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6721 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6781 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6841 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6901 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6961 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7021 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7081 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7141 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7201 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7261 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7321 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7381 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7441 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7501 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7561 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7621 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7681 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7741 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7801 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7921 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7981 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tt
//
