LOCUS       sequence-441685-        7253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ser-2(prom1B) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY117
FEATURES             Location/Qualifiers
     source          1..7253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2928..2948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2973..2993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3015..3035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4844..4866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4966..4985
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5063..5081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5149..5253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5254..5322,5323..6114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5472..5491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6285..6873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6774..6793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7161..7182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7197..7202,7203..7220,7221..7227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7235..7251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7240..7253,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc ttcaaactgg
      181 gcgacaacat ttcatagatt ttccaaaaac tttaatatat gaagctcata atatcacaac
      241 ttttaataaa cagtaatata gggttccttt attggaattt attaaaattt cttgtttaaa
      301 attcatatac agggtggtcc ataattatga accaccatgt gcttaaaaaa gttataccat
      361 caggtttcaa acttagacaa ccttagaaac gttcgttaga ttttcgaaaa aaatttgaga
      421 aactaatagt aagaataaac caatgatcat cagagttagt ttcccatgtg ctttcgtgag
      481 aagttgtgaa atgtctaaat aaattcgaaa atcgcccaaa ttggatcaaa ttttcatatt
      541 ggcttgaaag gtggcttaaa aattttttga aatatttacg ctccacctat atcttgtgtt
      601 tttgcgtttt tccaaaaaga actttcatat aaatcaaact aatattagaa cttttacatt
      661 tacattcccg tttcaagaat tgtgctcgaa ttagaaatcc attggaaatc taaaatccgc
      721 agttaatcga atcgtccgtc acctaatatt atttccgatg aacgcttttt tttctgatga
      781 gcaactagac acattcattc ccaccacaat tttaaattaa aactttataa tcgaatatca
      841 acatatcagt ggtaagagga aattggaaat atttgctact tgccttcatt ttatttttat
      901 tccctcccca ctccttctcc ccaccatttt ttgctcctgt tcatcatcaa cactcactgc
      961 gaatcctgaa tccattcgac cgtgctcgaa tcattttttt ttatttcggc gaagaagtcc
     1021 acttttcgga catacactat taatcaattg cctcccccta aactatgtat tattcgaata
     1081 ttgtcaccgc gtcaagtagt cttagtttaa ttgacacatc aaacaccaag ttcaaggtgg
     1141 gctcaggggc actgaccttt tctaaatttg gaaataagta agcggggtga tgtcagcagt
     1201 caaatctggt gaataaaata tggaggtgaa acaaaaaata aacctcgagt tttttttttc
     1261 aaaattttat aaaatactct tctcaattac atcattattc aaaaacaaaa aaaaattgca
     1321 ctcaatctaa tggaatacat attttccaat ttccggtctg taatgtatag actagtacac
     1381 acgactagaa caaaagaatt taaccttttc ttttgcaacc gtgtgttgtt tttttttttt
     1441 gagtattttc tcgtaaatgg ttcaaagtta ttctacgtca ccggtttcgc aaaacatcga
     1501 aaaacagaga cttctgcaga ataaagcggg aattagttgt gtttcgttgt attccgtggg
     1561 aaatatctca tctttgaact agaaattctc tcataaagaa taattttgcc aataaacgaa
     1621 actctcggaa agaatgaggg aattcgtatg taaattgcca tctttcttgc tttatttcac
     1681 tttttatgaa agtaaaaagc aacttgtttt aatttgaatt tagcatattg ggggtcctca
     1741 cgtgatgtca gagtgtctca catcggcttg atctacctaa atccacaaaa aatgcggatg
     1801 aagagaagca gagtttttaa ctgtattcgc atgatgctga agtcactttt tttgggaaaa
     1861 aaaatccccg cattttctgt agatcaaacc gtaatgggac agcctggctc tacgtgggtc
     1921 ctgataacga attacttggt tacgtgaaaa aaaatgacat cgactcaaaa aattttgaat
     1981 tcaacgaaat ttttgaagtt tgatcagaaa aaaacaattt tgaaaatttt gaaaaatcca
     2041 agaattaaga aaaattgttt tttttttggc tcatattgaa cacaaaattc gtaaaggcgc
     2101 tattacatta taactatgtt tgtttttaaa agcgcttttt taaaagcaac tgtgcaaaat
     2161 aaacatacaa tttcccgtat taaaaaaaaa ttttgccaca atttctcaaa attttaaagg
     2221 tggattttgg agattctctc gccttggcac tataattttg aaataaattt tcctccccct
     2281 aacatacaat tcatcaaaac aaaatcccaa atgttacctt atctgaccta atttttggcc
     2341 aaaaccagga aggaaatagt tgagacaact cggcacgggt caagccgtca aattagtgcg
     2401 caacattcgc aaacgactag tagaaacact tggccaacag acacagagag aagacaaaaa
     2461 acgacgatga aattaaagat ggggtttgac ttttcttctc tgttctgttt cttttttggt
     2521 tggttttttt gttgccacta gttttggctc taaagactcc gaaagctcca actccaatca
     2581 agaaactgat gaatcgcctt ccattctatt gtttgaaata ttatgcttgc tctttgcaga
     2641 gagcatcttt ttgagcagta atgatacaaa aaaagatgat gaatgaagaa catttgtcaa
     2701 gtcttttgct ttaaatctat gaaaaaaaga gaaagcccac tacttccact cattcgtttt
     2761 ctactcaact tgtctgattc aggaaatggc ccgggattgg ccaaaggacc caaaggtatg
     2821 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgga gggtaccggt
     2881 tttcccagtc acgacgcgtg gtaccgagct cagaaaaaat gaccgctcca aagaagaaac
     2941 gcaaagtacc gagctcagaa aaaatgacgg cacctaaaaa gaaacgcaaa gtaccggtcg
     3001 ccgagaagac agccccaaaa aagaagcgta aagttccacg cgtcatggga gtaagtttaa
     3061 acagttcggt actaactaac catacatatt taaattttca gcgcgctgtc tccaagggag
     3121 aggagctcat caaggagaac atgcacacca agctctacat ggagggaacc gtcaacaacc
     3181 accacttcaa gtgcacccac gaaggggagg gcaagcccta cgagggcacc cagaccaaca
     3241 ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacctgct
     3301 ttatgtacgg gagcaagacc ttcatcaacc acacccaagg aatcccagac ttcttcaagc
     3361 aatccttcgt aagtttaaac atgattttac taactaacta atctgattta aattttcagc
     3421 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg cttaccgtta
     3481 cccaggacac cagcctccag gacggctgct tgatctacaa cgtcaagctc agaggggtga
     3541 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccagtaccg
     3601 agaccctgta ccccgctgac ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg
     3661 tgggcggggg ccacctgcac tgcaacctga agaccacata cagatccaag aaacccgcta
     3721 agaacctcaa gatgccagga gtaagtttaa acatatatat actaactaac cctgattatt
     3781 taaattttca ggtctacttt gtggaccgca gactggaaag aatcaaggag gccgacaatg
     3841 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     3901 tggggcacaa acttaatggc atggacgagc tgtacaagta agcgccggtc gctaccatta
     3961 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
     4021 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
     4081 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     4141 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
     4201 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     4261 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     4321 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     4381 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     4441 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     4501 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     4561 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     4621 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     4681 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     4741 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     4801 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4861 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4921 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4981 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5041 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7021 gagcgcagcg agtcagtgag cgatgaagcg gaagagcgcc caatacgcaa accgcctctc
     7081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7141 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7201 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
