LOCUS       sequence-441731-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAC3-tagged VPS35L in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Laulumaa S, Kumpula EP, Huiskonen JT, Varjosalo M
  TITLE     Structure and interactions of the endogenous human Commander 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 
            10.1038/s41594-024-01246-1. Epub 2024 Mar 8.
  PUBMED    38459129
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01246-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-06"; volume: "31"; issue: "6"; pages: "925-938"
COMMENT     Sequence Label: MAC3_C_VPS35L
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3728..3752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3799..3822
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3859..3882
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3913..3939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3913..3939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4496..4513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4502..4726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4859..4878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5010..5057
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5065..6087
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5065..6087
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6217..6338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6254..6273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6308..6327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6387..6403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6387..6403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6400..6422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6411..6427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6435..6441,6442..6459,6460..6465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6618..6635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6789..7377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6869..6888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7548..8339,8340..8408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8171..8190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8409..8513)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8588..8607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catggccgtc
      841 tttccttggc actccaggaa taggaactac aaagctgaat ttgcatcatg ccgactggag
      901 gctgtaccat tggagtttgg ggactatcac cctctgaaac ccataactgt cacagagtca
      961 aagacaaaga aagtgaaccg gaaaggaagc acttcttcca cgtcctcctc ctcctccagc
     1021 tccgtggtgg acccgctgag cagcgtcctc gatgggactg accccctctc catgtttgca
     1081 gccactgctg accccgcagc cttggcagct gccatggaca gctccagaag gaaacgtgat
     1141 agagatgata actccgttgt aggatcggat tttgagcctt ggaccaacaa acggggagaa
     1201 atccttgccc ggtacaccac taccgaaaag ctgtctatta atctgtttat gggatctgaa
     1261 aaaggcaaag ctgggactgc cacattggca atgtcagaga aggtgcggac ccggctggag
     1321 gagctggatg actttgagga gggttcccaa aaggagctgt tgaacttgac tcagcaggat
     1381 tacgtgaacc gcatagagga gctcaaccaa tcgctgaagg atgcctgggc ctcagaccag
     1441 aaagtgaagg ctctaaaaat agtcatccag tgttcaaagc ttctttcaga caccagtgtt
     1501 attcagttct acccaagcaa atttgtcctt atcaccgaca tacttgatac atttggaaag
     1561 ctcgtgtacg agcgcatctt ttccatgtgt gtggatagcc gcagcgtctt accagatcac
     1621 ttttctccag agaatgcaaa tgacacggcc aaggaaacat gcctaaattg gtttttcaag
     1681 attgcctcca tcagggaact cattccaaga ttttacgtgg aggcatccat cctgaaatgt
     1741 aacaaattcc tctccaaaac gggaatttca gagtgcctgc cccggttgac atgcatgatc
     1801 agagggatcg gagacccact agtgtcggtg tatgcccgtg cctacctgtg ccgggtggga
     1861 atggaagtgg ccccacatct caaagaaacc ctaaataaga acttttttga cttcctcctt
     1921 acgttcaaac agattcatgg ggatacggtc cagaaccagc tggtggtcca aggagtggag
     1981 ctcccatctt acctcccctt gtacccgcct gccatggact ggatcttcca gtgcatctcc
     2041 taccatgccc ccgaggctct gctgaccgag atgatggaaa ggtgtaagaa actaggaaac
     2101 aatgccttgc tgttgaattc tgtgatgtct gccttccggg ctgagttcat cgccacaagg
     2161 tctatggatt tcattggcat gattaaagag tgtgatgaat ctggtttccc caagcatctt
     2221 ctttttcgat cactgggatt aaacttggcc ttggctgatc ctcctgagag tgaccgactt
     2281 cagattctca acgaagcttg gaaagtcatc actaagctga agaacccaca ggactacatt
     2341 aattgtgccg aagtgtgggt ggaatacacc tgcaagcatt tcacgaaacg agaggtgaat
     2401 accgttttgg cagatgtcat caagcacatg actccagatc gtgcatttga agattcctac
     2461 ccccagcttc agttaataat taagaaagtt attgcccact tccatgactt ctcagttctt
     2521 ttctcagtgg aaaaatttct gccgtttctg gacatgttcc aaaaagagag tgtgcgggtg
     2581 gaggtttgca aatgcatcat ggacgccttt atcaagcatc aacaagagcc caccaaggac
     2641 ccggtcatct tgaatgccct tttgcatgtt tgcaagacca tgcatgactc tgtgaatgca
     2701 ctcactcttg aggatgagaa aagaatgctg tcatatttga ttaatggatt tataaaaatg
     2761 gtttcctttg gccgtgattt tgaacaacag ctgagttttt atgttgagtc caggtcgatg
     2821 ttttgcaatc tggagcctgt tcttgtgcag ttgattcata gtgtgaaccg gttggcaatg
     2881 gagacaagaa aagtaatgaa aggaaatcat tccagaaaga cagctgcatt tgtccgggcc
     2941 tgtgttgcct actgcttcat caccatcccc tccctggcgg gcatcttcac acgtctcaat
     3001 ctctacctgc attctggtca ggtggccttg gccaaccagt gcctctccca agctgatgct
     3061 tttttcaaag ccgctataag ccttgttccg gaagttccaa agatgattaa tattgacggg
     3121 aagatgcggc catcggaatc gttccttctg gaattcctct gcaatttctt ttctacttta
     3181 ttaatagttc cggatcatcc tgaacatggg gtcctgtttc ttgttcgaga gcttctcaac
     3241 gtgatccagg actacacctg ggaggacaac agcgatgaga aaatccgcat ctacacctgc
     3301 gtcctgcatc tcctctccgc catgagccag gagacgtacc tttaccacat agacaaagtg
     3361 gactccaacg acagcctcta cgggggagac tccaagttcc tggcagaaaa caacaagctg
     3421 tgtgagacgg tgatggctca gatcctagag catctgaaaa ccctggccaa ggacgagacc
     3481 ctgaagcgcc agagctcgtt gggcctttcc ttctttaaca gcatcttggc ccatggggac
     3541 ctacgcaaca acaagctcaa ccagctctcc gtcaacctgt ggcacctggc acagaggcac
     3601 ggctgtgcag acaccaggac catggtgaaa acgctagaat acatcaagaa gcaaagcaaa
     3661 caaccagaca tgactcatct gacggagctg gccctcagac tccctctgca aacaaggacc
     3721 tggatccacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     3781 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     3841 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     3901 accatggcca gctaccccta cgacgtgccc gactacgccg acttcaagaa cctgatctgg
     3961 ctgaaggagg tggacagcac ccaggagaga ctgaaggagt ggaacgtgtc ctacggcacc
     4021 gccctggtgg ccgacagaca gaccaagggc agaggcggcc cgggcagaaa gtggctgagc
     4081 caggagggcg gcctgtactt cagcttcctg ctgaacccca aggagttcga gaacctgctg
     4141 cagctgcccc tggtgctggg cctgagcgtg agcgaggccc tggaggagat caccgagatc
     4201 cccttcagcc tgaagtggcc caacgacgtg tacttccagg agaagaaggt gagcggcgtg
     4261 ctgtgcgagc tgagcaagga caagctgatc gtgggcatcg gcatcaacgt gaaccagaga
     4321 gagatccccg aggagatcaa ggacagagcc accaccctgt acgagatcac cggcaaggac
     4381 tgggacagaa aggaggtgct gctgaaggtg ctgaagagaa tcagcgagaa cctgaagaag
     4441 ttcaaggaga agtgatgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     4501 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4561 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4621 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4681 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4741 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta
     5041 gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     5101 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     5161 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     5221 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     5281 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     5341 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     5401 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     5461 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     5521 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     5581 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     5641 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     5701 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     5761 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     5821 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     5881 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     5941 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     6001 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     6061 agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc
     6121 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6181 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6241 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6361 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7561 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7621 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7681 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7741 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7801 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7861 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7921 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7981 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8041 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8101 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8161 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8221 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8281 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8341 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8401 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8461 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8521 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8581 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8641 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8701 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     8761 gggccagata tacgcgtt
//
