LOCUS       sequence-441738-        8773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ser-2(prom3) fluorescent neural reporter driving nuclear mNeptune2.5
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY118
FEATURES             Location/Qualifiers
     source          1..8773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4448..4468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4493..4513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4535..4555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6364..6386)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6486..6505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6583..6601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6669..6773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6774..6842,6843..7634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6992..7011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7805..8393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8294..8313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8681..8702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8717..8722,8723..8740,8741..8747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8755..8771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8760..8773,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc atgcttgttc
      181 tagtgatcaa aattgtcact tataaagtaa aagtttagta aattaactgc taaagaatat
      241 aaagctaaca cagcaaaaca tgtgaatgat ccaacgataa tcaccaccca caacatagct
      301 ttgagttgat tatttgagtt tttaaatcaa aatcgtcttt ttaaaacagg ataaacccga
      361 cgaaattcaa aaaattccca gaaagtgaaa aaaaatcaag caagtatgtg tctttgattt
      421 ttgtagtttt tttttacaaa tttcaaaaga tctagtatcc taaattatac cgtatattcc
      481 ctattagtat cgcccccctc actcgactta tcgtttatat cttggttgtc tttgatcata
      541 ttttaaaaat tcaaacgaca aaatcttttt caaatctatc gttatgctct ggtttttgaa
      601 gatatcaaaa atcttctgaa accgctgaaa tattagccga caaagtcgat taatgggggg
      661 ccataataat agggaatagt gtttttccca tattccctaa ttgttttcca aatatcaatt
      721 ttcacagttt taatttaatt gcgcttacta catagtttta actaagtttt aatattcgaa
      781 actagctgtt acaaaaaggt tatgaaatta cattttcaga cgatttctga atttttaaac
      841 attcaaaagg tcttgctata ttgtttgatc caaataaaca aaaagcctgt acaagaaaca
      901 tatcttattt catcataaat aatgttgttc tattttgaaa taattacata attaagttga
      961 aaaattgtca gaagccggaa aaaaacacga atcgactttc ccagcaagaa tatcagaaaa
     1021 cctgttgagc aaaaaaaaca atctcgttgt tgaaaaaacc agatagctgg aagaaattga
     1081 aaataaaaag acaacacaca tatatactaa aaacatgagg caaaagaact gtctattgaa
     1141 acacgaaacg aggagaaaaa aaaaaaataa aaacggcctt gtgattgttc agttgagttt
     1201 tgtttcacac ggtttttacg atcaatgcaa agaaaaaaaa aggattcaat aatggtggaa
     1261 gaaatattcg tggaagtaca actatgtact acaacttatg aatttgaatt tcattcccga
     1321 aaatttgtcg gaaaattgtg taaaaggggt catatagata ataaccgatg tggggggaga
     1381 aaggatgaag aagtggtggg aggcaaaaca aagatgaatc cgaaaaggat gcagcaattc
     1441 agaacagatg catcccggaa aatgaaaacg aaggtcatcg ctatttctta ccttttcacc
     1501 aaaatctttc ttcttcactg atccagggtc agaaatttga aattttgaag gacaacagtg
     1561 ttgccatagc aggaactggg ggctggggct ggggggagaa aatttatttc tcacgtcgtt
     1621 tttctcaacc ttcttcagtc tcctagatat ctcctagaaa cctttgagtt atacgagtga
     1681 agagacggaa aagccacaga ataagggaaa aatgtaagta agagtggtgg agagccaagg
     1741 aaatgtgaca aattaataac acgacggcct acaactcact ttgttataat aattttaatt
     1801 tgaaatcttc tttcttgcaa aaagagtact agtgaaggac ggatgaagaa cgatggaata
     1861 cttgaaaatc tgctcagatc ttcttctttc ctcctcgaaa ttagagttaa tccaaaagat
     1921 aaacagttgt aagtggtgtg tgaatcttca agagagccgg agatgacagt ctcagaggtg
     1981 agtcactgtt tttttttgtt ggattgtgct gggcttagct gctagccgga tgtaagaata
     2041 ttgaaaaaat gaatataaaa agtttaatga tataaaccgg tagcgagatg atatataagt
     2101 tcagatgtgt tgataaatag ggtttttcgt ttaaatgtac tgataattat tgaaaatttt
     2161 aaattttcta gaaaatcagt tcaaaaaatt ttgattttgt aataacaaaa aagttcagtc
     2221 aaacgttcag gcagttgcca tgagtttagt aaagctagac tttttgagaa cattttttgt
     2281 tgaaataatt gttttaattt taaaatgttt agttcaaata actagaacat acataagtag
     2341 aacataaaat ttgaataaaa ttgtcgtctt ccaaaattgt aaaattatat tgcaaaaact
     2401 gtgtaactca aactgctgat caaaatattt attgaagttt aacagagttt aaaacacaaa
     2461 attatagtga agaaatgcac agaaaatgca tccaaactca attatttcct agaatgaaga
     2521 attttaaaaa tgatgttata acaaaattct tttcaattca aaaactagat tgtatttaca
     2581 acgtcggaat gattaaaaca catttagaat ataaaattct taaaataaat taaaattttg
     2641 tggactggac tttttggaga cttggcgaat tatggttatt ccgaaacgct gtcgacttca
     2701 actgtaggcg aaatacacgc gcgcggaact cgtaagatgt cggcggcctt atattgggtg
     2761 gggtcccgat ttttgccttt ttcccgtgag tggacgagca aaattcgggg cttctttggg
     2821 cgtacacgcc aagcagtttt attattttga gtgtatagct cttaaaaaac tgtaaaaatg
     2881 tgtttgaaaa agatatgcta tgaattttga tcattttatc cactgtactg aaactcgaac
     2941 ataaaaacca aaattatacg aaaaaatcgt ttggagacgg cgattctaac ttttggtgat
     3001 gaaaaactta tcgaaatgtt acatttcgaa tctttgatat cagaaaaaaa gtcactgatc
     3061 atcttctgaa ataaattaac aagaaagtat tcctctagta tcaaaatgtc tctagtggct
     3121 ttctcaaaga cattaatccc catatctcat agttcagacg aactttccac agtaaacaat
     3181 actagacgtt cagaatatga aatttaatca tgttttatat gaaaaaagtt gctccattca
     3241 attttaatac tcgacaaaaa aggaaccaag ttccttagtg caaaaattcc aaaaatctaa
     3301 aacaggttcc actgagcttt ggcaaaatga ttagaagagc atttcaatgc tcttctaata
     3361 tgtttctagg tgttattttt tttctcaatt tttttttgta actttcttgt tttagcaaca
     3421 acaaaaatta tttcatttga aattacagaa acagaaattc tcaaaatgct gtttccaaaa
     3481 atcaggtata atttgttcac acaattaata atgtttctag aaaatagttt tattttgacg
     3541 caactaagaa acataaaaac atcttttaat cagtcagttt taatcagggg cagatagctc
     3601 agccggtagc gctggccgct agcaatctgg aggtcacgag ttcaagtccc gcctcacccc
     3661 ctatgctcac ccatcatcta ttaggaagtg gagcaatccc caactagatt atcggccaca
     3721 gtccccggct aggacgtggc ttaaattaca gctcagtggg agcaccacca ggcagtgtac
     3781 ctgaatccca gatccgcagt acatagcact tgaagaacgg atcgtccttt aatccttttt
     3841 ttttaaatct cgtttcaaaa ttattttaaa cccatttttt tcaaattgtt acttgaattc
     3901 agtgtttctg aatttattac agccatatgt gttttgtgat ttctgatcca tctcgattcg
     3961 gtttccactt ctcatatcca tttctcaacc cgtttttttt gtattctacc tttttcctac
     4021 caactcatgt caatttcgca caacataaca aatcacaacg tctaaaagcg aagcaaatga
     4081 atgtaaaaga aggttatcaa tcagagtctc ctcgggctcc ttccttttga ggtttgatga
     4141 cgcgaaaaat cggaagggcc ttgcgttgtc ctccggccag tagcaattca tttcgtagac
     4201 gtttttttcc cctgctacat tctgacgaca ataatattat ttttgttggc taaatttact
     4261 atagctaatt attcattttg gcatattttt gtgacatcac aacacataac ccgggattgg
     4321 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4381 gacccttgga gggtaccggt tttcccagtc acgacgcgtg gtaccgagct cagaaaaaat
     4441 gaccgctcca aagaagaaac gcaaagtacc gagctcagaa aaaatgacgg cacctaaaaa
     4501 gaaacgcaaa gtaccggtcg ccgagaagac agccccaaaa aagaagcgta aagttccacg
     4561 cgtcatggga gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     4621 gcgcgctgtc tccaagggag aggagctcat caaggagaac atgcacacca agctctacat
     4681 ggagggaacc gtcaacaacc accacttcaa gtgcacccac gaaggggagg gcaagcccta
     4741 cgagggcacc cagaccaaca ggattaaggt ggtggaggga ggccccctgc cgttcgcatt
     4801 cgacatcctg gccacctgct ttatgtacgg gagcaagacc ttcatcaacc acacccaagg
     4861 aatcccagac ttcttcaagc aatccttcgt aagtttaaac atgattttac taactaacta
     4921 atctgattta aattttcagc ctgagggctt cacatgggag agagtcacca catacgaaga
     4981 cgggggcgtg cttaccgtta cccaggacac cagcctccag gacggctgct tgatctacaa
     5041 cgtcaagctc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     5101 cggctgggag gccagtaccg agaccctgta ccccgctgac ggcggcctgg aaggcagatg
     5161 cgacatggcc ctgaagctcg tgggcggggg ccacctgcac tgcaacctga agaccacata
     5221 cagatccaag aaacccgcta agaacctcaa gatgccagga gtaagtttaa acatatatat
     5281 actaactaac cctgattatt taaattttca ggtctacttt gtggaccgca gactggaaag
     5341 aatcaaggag gccgacaatg agacctacgt cgagcagcac gaggtggctg tggccagata
     5401 ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaagta
     5461 agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca
     5521 tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc
     5581 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
     5641 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     5701 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     5761 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     5821 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     5881 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     5941 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     6001 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     6061 ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac
     6121 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     6181 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     6241 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     6301 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6361 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6421 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6481 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6541 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     6601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgatgaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8761 gcggataaca att
//
