LOCUS       sequence-441740-        7429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nsf-1 fluorescent neural reporter driving nuclear TagRFP-T 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY106
FEATURES             Location/Qualifiers
     source          1..7429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3033..3053
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3078..3098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3120..3140
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5020..5042)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5142..5161
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5239..5257)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5325..5429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5430..5498,5499..6290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5648..5667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6461..7049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6950..6969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7203..7220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7337..7358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7373..7378,7379..7396,7397..7403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7411..7427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7416..7429,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cgtgacatta
      181 cccggcacaa aaaaacattt cgaaccggac aatgaaatta cacgagaaat aagagggaag
      241 cagtaagatc acagagtatg ccgcattggg aataatgaca aactccacgt tcttaagtag
      301 acacaaaata cattacaata tagaggaaat atgccgagag taggaaataa acatactatt
      361 cagttgttct tttaagcaat agaagcaact aaatagctag ttgaaagact tcgtgtagca
      421 ttgagaacaa aaaacacatt cagaagagaa aagcaaataa ttttaggccc tctaattcta
      481 aaaatcctag actaaaaagg aatgaaaatt ataaaacgtc aagaacaaaa tggcttatga
      541 ctgagatata atgctcctct caatatgcta taattattga ttaactgaat ttcccataaa
      601 ctgacattgc tttcaatgtt ttgaaacaaa aagggctgtt gcattgtgag aataacacta
      661 tttttctatt gaaattgaca gaaactaagc gaattgtaca tttcagtgat acatttacaa
      721 ttgcggaacg aaataataag cgagaacgca gcaaaaaatg agccgaatcg ttgagcgcgg
      781 aacgcgtcgc attcgaaaag tcgatagttc catggagcgc atttgctcta ctgacacgtc
      841 acggaatctg tgaataaagt ttgaaagaaa tgcgttcaat gccgaaaacg aaaacttcgc
      901 tcacatttta agtgagaaag aatagcagct aaattgttat aaatattcta aaacatagtg
      961 ttaaaggatt ttaactactt ttcataattc tgtttcgccc gactcacaca attcaaaaat
     1021 tgaagtggtc tcataataca cgtttcccgg attttcatga gtttttaaga ttttatttgc
     1081 aatttaatat ttctaatctg ctgtttttat cacaaatgcg aaaaatataa agtttctgat
     1141 gcttaaatat tgatttcgtc aaaatatttt ttgtaaaacc acatcagctg ctaaataata
     1201 aattcttttt attttttttt taaagttcat attatttgtc cagttacaat ttattagttg
     1261 cgaaattcat atgcaacgtg tccttggacg agttttcagt cagacatttt ctcggtttcg
     1321 ttttgtttta ttgggaactg tgctttcaaa cttttaacta acgtttacta ataagtaata
     1381 tggatgaact tgaactggaa ttttcttaaa atcagggttt tatttgaaca tttaacattt
     1441 ttaaattcgt taaaatttta ttcatgacgg tttgacatga cttaattttt ttccagttct
     1501 agccactcca tattatttta atgtttactt gcatgaatat ttgagaaata ccaaaagttg
     1561 ctcatcattt cttagtttgc ttctttctcc atcatcaaaa agtccaccct tttcacttcc
     1621 tcccctccca ttttccggct cttttttcac gtatctcccc catccgtctg tcctcttctc
     1681 caactcttcg tgtcttttca tgcccaaagt cctacatcga tttgcgcagg aaaaagcctc
     1741 cgagtgccga aacaacgtct tcttcttctt cgtatttttc ttgttttcaa cgcactgctc
     1801 ctctcgttct gcagtttttg ttagcatctt ccccatctct tcgtattttt ttgcttctca
     1861 tttccgtttt ctctccattt acttctttcg gcctcttttc ggtcctccgc tttttgtttt
     1921 ctgttttgat gccattattc ccacgttatt ctggagaaca tttccataaa ccaaggaaag
     1981 tcatgaatta ctgaaataat gacaggataa gctgtttctt cagttttatc cgaaacaatt
     2041 caaaccttaa tactttgaaa atttaattat taaattcagt gtaatcaaac ccctgaaatc
     2101 tgactctttc tcactttcgg tatcctaaaa atgaaaattg tgtctgcccg atttttcctt
     2161 cgttttgtct gttatcagat tatatgtccc agcgcgcatg cgttttctaa tagagttaca
     2221 ttattttttg tctagagctt catgagaaaa aaactaaaaa ttctaaaata aatttccttt
     2281 catgtttcat gcccattaat ctaaaaatta cgtttcggtc ctttccttta gaaaaatggc
     2341 tcaaaaacct ccaaatgcat gcttttcgcc aagaaatcgg agccgccaca aacggcgcga
     2401 cttccactca atttttctcc gttttccgaa taattttgtg tgcatttgtg tgtgtctcgt
     2461 tgagggtgga cgtgtccatg tattcgaatt caattttcct cctaccatgt ttgtgctttc
     2521 caatcagctc cgtccacttt atggtgtagc gttaaattat aggatagaat ggacactgag
     2581 acttccttcc gcagttcctg ggtgaacttt ttggaatctc ttgagacaat taacggttaa
     2641 acttatctaa gaacgagttc gaacattatc tggtcttatt catctatggg ttcttttcag
     2701 gccttttcta aaaaacttaa tttttttgtt tttctatatt tcggaaaacc aaaaaagtcg
     2761 aaagctaatg cgatgcctca attctccaaa aaaaaaagac atttctgcgg cttgtattat
     2821 ggaaaaatcg aaattttttt tggcgacgcc cctggttcat atcatttttt tcgggtacga
     2881 aaaattaatc gaggcccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
     2941 acataacata gaacattttc aggaggaccc ttggagggta ccggttttcc cagtcacgac
     3001 gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa gaaacgcaaa gtaccgagct
     3061 cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc ggtcgccgag aagacagccc
     3121 caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag tttaaacagt tcggtactaa
     3181 ctaaccatac atatttaaat tttcagcgcg cttccaaggg agaggagctc atcaaggaga
     3241 acatgcacat gaagctctac atggagggaa ccgtcaacaa ccaccacttc aagtgcacct
     3301 ccgagggaga gggaaagcca tacgagggaa cccaaaccat gcgtatcaag gtaagtttaa
     3361 acatatatat actaactaac cctgattatt taaattttca ggtcgtcgag ggaggaccac
     3421 tcccattcgc cttcgacatc ctcgccacct ccttcatgta cggatcccgt accttcatca
     3481 accacaccca aggaatccca gacttcttca agcaatcctt cccagaggga ttcacctggg
     3541 agcgtgtcac cacctacgag gacggaggag tcctcaccgc cacccaagac acctccctcc
     3601 aagacggatg cctcatctac aacgtcaagg taagtttaaa cagttcggta ctaactaacc
     3661 atacatattt aaattttcag atccgtggag tcaacttccc atccaacgga ccagtcatgc
     3721 aaaagaagac cctcggatgg gaggccaaca ccgagatgct ctacccagcc gacggaggac
     3781 tcgagggacg taccgacatg gccctcaagc tcgtcggagg aggacacctc atctgcaact
     3841 tcaagaccac ctaccgttcc aaggtaagtt taaacatgat tttactaact aactaatctg
     3901 atttaaattt tcagaagcca gccaagaacc tcaagatgcc aggagtctac tacgtcgacc
     3961 accgtctcga gcgtatcaag gaggccgaca aggagaccta cgtcgagcaa cacgaggtcg
     4021 ccgtcgcccg ttactgcgac ctcccatcca agctcggaca caagctcaac ggaatggacg
     4081 agctctacaa ggcctaggat accggaattc caactgagcg ccggtcgcta ccattaccaa
     4141 cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc
     4201 tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta
     4261 agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     4321 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     4381 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     4441 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     4501 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     4561 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt
     4621 cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat
     4681 ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt
     4741 ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca
     4801 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     4861 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     4921 atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg
     4981 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5041 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5101 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     5161 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5221 catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5281 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
