LOCUS       sequence-441744-        5666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nsy-5 (prom3) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY107
FEATURES             Location/Qualifiers
     source          1..5666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1341..1361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1386..1406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1428..1448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3257..3279)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3379..3398
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3476..3494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3562..3666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3667..3735,3736..4527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3885..3904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4698..5286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5187..5206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5574..5595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5610..5615,5616..5633,5634..5640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5648..5664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5653..5666,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aagaaagaac
      181 attttaaaca tggggcatcg tggccaaaat aacaaagtgt gctcctaatt cgccatactt
      241 tacgcgcaaa gttaaaaaat tttattggaa aagaacatta atatgcttga tttaatttta
      301 cgctaaaatt ttgaattttt gaaaaaaaat ttcaaatttt aagttttcaa aaaaattttt
      361 tttttcaatt tcaaattttc aaaaaaaaat ttataccaaa atacctaccc aaaaaaagtt
      421 actttaaaaa tcaatatagc gcaataactt ctggaaattt gacgaatttt ttttcaaagc
      481 agtaatcaag aaacaaattc aaaaaaatca aatactaggc tcattcaaga ataaaaacaa
      541 atttaaattt ccaattttca gtagaaattt tttccaactt ttgtcttttt gcaaacgatg
      601 ccccacagtg agcttccacg tcaccactta tcccatttcc ttaccgccac tttgcgcctt
      661 ctaatccgtc catttcaaaa ctcgagtact tttctttctc ccggcctttc tccatgtcat
      721 cccacacctc ttcccgtgct aattgccacg gaaatagtgt agaagcctac gagacgcagc
      781 acacaacgtt ttgcttatta gtacgttcat tgaaatgagc agcagaagag atgaagtcgt
      841 ccttttgaca cataccctac tttagattat tattattatt actactacta ctactataga
      901 attcctatta tacatctggt aactccaata attcgacgaa cgtataataa gaaaattatt
      961 aagaaaagtg agtcgttccg atgacagatg ggaccttttt ttcaatatta ggaaaattat
     1021 ttttgaatca cctgagaatg tcgcatgccc acacagatac acaaacattt ttcaaaattt
     1081 ctgattttct aggttcgtgg gctagaaaac taaagaaaaa actcggccac caattttttc
     1141 cggtggccga gttttctgag tttctcggcc actaagtggg ggctaggctg tcccaccacg
     1201 ttcccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     1261 acattttcag gaggaccctt ggagggtacc ggttttccca gtcacgacgc gtggtaccga
     1321 gctcagaaaa aatgaccgct ccaaagaaga aacgcaaagt accgagctca gaaaaaatga
     1381 cggcacctaa aaagaaacgc aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc
     1441 gtaaagttcc acgcgtcatg ggagtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagcgcgct gtctccaagg gagaggagct catcaaggag aacatgcaca
     1561 ccaagctcta catggaggga accgtcaaca accaccactt caagtgcacc cacgaagggg
     1621 agggcaagcc ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc
     1681 tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca
     1741 accacaccca aggaatccca gacttcttca agcaatcctt cgtaagttta aacatgattt
     1801 tactaactaa ctaatctgat ttaaattttc agcctgaggg cttcacatgg gagagagtca
     1861 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
     1921 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
     1981 agaagaaaac actcggctgg gaggccagta ccgagaccct gtaccccgct gacggcggcc
     2041 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
     2101 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgcca ggagtaagtt
     2161 taaacatata tatactaact aaccctgatt atttaaattt tcaggtctac tttgtggacc
     2221 gcagactgga aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg
     2281 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg
     2341 agctgtacaa gtaagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     2401 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     2461 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     2521 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     2581 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     2641 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     2701 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     2761 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     2821 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     2881 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     2941 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     3001 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     3061 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     3121 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     3181 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     3241 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     3301 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     3361 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3421 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     3481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3901 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4201 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgatgaa
     5461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5521 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5581 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5641 tgtggaattg tgagcggata acaatt
//
