LOCUS       sequence-441761-        2625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal expression IRES-containing bicistronic vector for 
            generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S,
            El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr
  TITLE     Development of covalent chemogenetic K(2P) channel activators.
  JOURNAL   Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 
            10.1016/j.chembiol.2024.06.006.
  PUBMED    39029456
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2024.06"; journalName: "Cell Chem Biol"; date: 
            "2024-07-18- 18"; volume: "31"; issue: "7"; pages: "1305-1323.e9"
COMMENT     Sequence Label: pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..2625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1264..1316
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(1318..1893,1894..1896,1897..1905)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1486..1503)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1713..1732
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1906..1908,1909..1911,1912..2625)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1906..1908,1909..1911,1912..2622)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1951..1972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2212..2231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2559..2580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtacccat acgacgtgcc agactacgct gcggcccctg acttgctgga tcccaagtct
       61 gctgctcaga actccaaacc gaggctctca ttctcctcaa aacccaccgt gcttgcttcc
      121 cgggtggaga gtgactcggc cattaatgtt atgaaatgga agacggtctc cacgattttc
      181 ctggtggtcg tcctctacct gatcatcgga gccacggtgt tcaaggcatt ggagcagcct
      241 caggagattt cccagaggac caccattgtg atccagaagc agaccttcat agcccagcat
      301 gcctgcgtca actccaccga gctggacgaa ctcatccagc aaatagtggc agcaataaac
      361 gcaggaatta tccccttagg aaacagctcc aatcaagtta gtcactggga cctcggatgc
      421 tctttcttct ttgctggtac tgttatcaca accataggat ttggaaacat ctccccacga
      481 actgaaggtg gaaaaatatt ctgcatcatc tatgccttgc tgggaattcc cctctttggc
      541 tttctactgg ctggggttgg tgatcagcta ggaactatat ttggaaaagg aattgccaaa
      601 gtggaagaca catttattaa gtggaatgtt agtcagacga agattcgtat catctccacc
      661 atcatcttca tcctgtttgg ctgtgtcctc tttgtggctc tccctgcggt catattcaag
      721 cacatagaag gctggagcgc cctggacgct atctattttg tggttatcac tctgacgacc
      781 attggatttg gagactacgt ggcaggtgga tcagacattg aatatctgga cttctacaag
      841 cctgtggtgt ggttctggat cctcgttggg ctggcctact ttttcgctgt tctgagcatg
      901 attggggact ggctacgggt gatctctaag aagacgaagg aagaggtggg agagttcaga
      961 gcgcatgccg ctgagtggac agccaatgtc acggccgagt tcaaggaaac gaggaggcgg
     1021 ctgagcgtgg agatctacga caagttccag cgtgccacat ccgtgaagcg gaagctctcc
     1081 gcagagctgg cgggcaacca caaccaggaa ctgactccgt gtcgtcgcac cctgtctgtg
     1141 aaccacctga ccagcgagag ggaagtcctg cctcccttgc tgaaggctga gagcatctat
     1201 ctgaacggtc tgacaccaca ctgtgctggt gaggacatag ctgtcattga gaacatgaag
     1261 tgactcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccgccc
     1321 ctctccctcc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     1381 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     1441 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     1501 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     1561 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     1621 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     1681 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     1741 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
     1801 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     1861 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     1921 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1981 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2041 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2101 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2161 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2221 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2281 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2341 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2401 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2581 accgccgccg ggatcactct cggcatggac gagctgtaca agtaa
//
