LOCUS       sequence-441765-       11516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on lentiviral vector for controlled expression of GDNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Gutekunst CA, Tung JK, McDougal ME, Gross RE
  TITLE     C3 transferase gene therapy for continuous conditional RhoA 
            inhibition.
  JOURNAL   Neuroscience. 2016 Dec 17;339:308-318. doi: 
            10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13.
  PUBMED    27746349
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2016-12-17"; volume: "339:308-318. doi"; 
            pages: " 10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13"
COMMENT     Sequence Label: pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
FEATURES             Location/Qualifiers
     source          1..11516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2029..2146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2185..2684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2220..2243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2617..2636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3382..3398
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3382..3398
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3416..3984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3435..3986
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3579..3596)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3806..3825
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3989..4609
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4062..4081)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4610..4630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4733..4927
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5066..5082)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5067..5083)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5097..5685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5150..5170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5568..5579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5823..5841,5842..5864,5865..5883,5884..5906,5907..5925,
                     5926..5948,5949..5967,5968..5990,5991..6009,6010..6032,
                     6033..6051,6052..6074,6075..6093)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6132..6312
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6337..6355)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6338..6355)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6645..6664)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6878..6897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6997..7019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7057..7075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7143..7247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7248..7316,7317..8108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7466..7485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8279..8867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8768..8787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9021..9038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9108..9128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9113..9442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9293..9428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10576..10641
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10771..10791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11216..11350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11253..11272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11307..11326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     2041 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2101 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac
     2161 tagcctcgac gatggtcgag taccgggtag gggaggcgct tttcccaagg cagtctggag
     2221 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2281 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2341 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2401 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     2461 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2521 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     2581 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     2641 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctct agcgggatcc
     2701 cgtcgagaat tagcttcgcc accatgaagt tatgggatgt cgtggctgtc tgcctggtgc
     2761 tgctccacac cgcgtccgcc ttcccgctgc ccgccggtaa gaggcctccc gaggcgcccg
     2821 ccgaagaccg ctccctcggc cgccgccgcg cgcccttcgc gctgagcagt gactcaaata
     2881 tgccagagga ttatcctgat cagttcgatg atgtcatgga ttttattcaa gccaccatta
     2941 aaagactgaa aaggtcacca gataaacaaa tggcagtgct tcctagaaga gagcggaatc
     3001 ggcaggctgc agctgccaac ccagagaatt ccagaggaaa aggtcggaga ggccagaggg
     3061 gcaaaaaccg gggttgtgtc ttaactgcaa tacatttaaa tgtcactgac ttgggtctgg
     3121 gctatgaaac caaggaggaa ctgattttta ggtactgcag cggctcttgc gatgcagctg
     3181 agacaacgta cgacaaaata ttgaaaaact tatccagaaa tagaaggctg gtgagtgaca
     3241 aagtagggca ggcatgttgc agacccatcg cctttgatga tgacctgtcg tttttagatg
     3301 ataacctggt ttaccatatt ctaagaaagc attccgctaa aaggtgtgga tgtatctgag
     3361 gatcaattca cgcgtggtac ctctagaact agtggatccc ccgggctgca ggaattccgc
     3421 cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3481 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3541 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3601 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3661 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3721 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3781 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3841 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     3901 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     3961 ttcctttgaa aaacacgatg ataataccat ggctagatta gataaaagta aagtgattaa
     4021 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     4081 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     4141 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     4201 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     4261 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     4321 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     4381 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     4441 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     4501 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     4561 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtcgc caaaaaagaa
     4621 gagaaaggtc gacggcggtg gtgctttgtc tcctcagcac tctgctgtca ctcaaggaag
     4681 tatcatcaag aacaaggagg gcatggatgc taagtcacta actgcctggt cccggacact
     4741 ggtgaccttc aaggatgtat ttgtggactt caccagggag gagtggaagc tgctggacac
     4801 tgctcagcag atcgtgtaca gaaatgtgat gctggagaac tataagaacc tggtttcctt
     4861 gggttatcag cttactaagc cagatgtgat cctccggttg gagaagggag aagagccctg
     4921 gctggtggag agagaaattc accaagagac ccatcctgat tcagagactg catttgaaat
     4981 caaatcatca gtttaaggat ctcgactagc tagtagctag ctagctagtc gagctcaagc
     5041 ttcgaattcg atatcaagct tatcgcgata ccgtcgacct cgactagtca tatgataatc
     5101 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5161 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5221 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5281 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5341 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5401 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5461 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5521 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5581 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5641 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg tacgtatggc
     5701 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5761 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct cgagtttacc
     5821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5881 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     5941 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     6001 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     6061 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcggatc gatctgcttt
     6121 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6241 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6301 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
     6361 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
     6421 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
     6481 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
     6541 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
     6601 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     6661 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     6721 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     6781 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     6841 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     6901 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7021 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7081 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7621 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8941 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9001 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9061 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     9121 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9181 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9241 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9421 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     9481 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     9541 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     9601 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     9661 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     9721 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     9781 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     9841 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     9901 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     9961 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    10021 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    10081 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    10141 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    10201 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    10261 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    10321 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    10381 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    10441 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    10501 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    10561 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    10621 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    10681 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    10741 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    10801 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    10861 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    10921 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    10981 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    11041 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    11101 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    11161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    11221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11341 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    11401 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    11461 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtag
//
