LOCUS       sequence-441784-        6816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mgl-1 fluorescent neural reporter driving nuclear CyOFP1 expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY104
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2175..2195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2217..2237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4407..4429)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4529..4548
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4626..4644)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4712..4816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4817..4885,4886..5677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5035..5054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5848..6436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6337..6356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6590..6607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6724..6745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6760..6765,6766..6783,6784..6790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6798..6814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6803..6816,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ttgcagaact
      181 tggagacctt caatattgat atctatctgt tatcagaaat tcttatggag cagtattcac
      241 ttctttaaca aaaatcacat tcatgaggca aacatttcgg gtaaggtatg tttttatttt
      301 ccaacacatg caaatcttgc ttctagaaag aataaaagtg cgcccttatt gagaattatg
      361 gaaatttttg tcgcgtgcga ccggcttctg atgtagcgtt tgaggagagg agcagatgaa
      421 ggcaggtcct tccggcgccg cccggatttg gattccttct ttgctgctta ctcctactgc
      481 tccgaaaatt ggctagaggt cgacgaaatg ggtggagacc ggcggtcccc ttcagaaaac
      541 gacagacatg caaacgacac atatgaatgt atatagggaa caagagtttc catactcata
      601 gtgctcatta gaatagcacg gatcgtgttt cgcctctcgc cttgttaacc gaatctgccc
      661 ccgtgtgccc cggctgcttg tgttgtgtca cacaagacta acgccctctt atcctttcca
      721 ttctcttaaa atccatttct agagttgaaa actttatttt tattcaatac tcaaatcatg
      781 gtaccggtaa gcgaaagttt attttaataa ataaatgggg aaagcccatc acaattgggc
      841 tggtaatggc gacaattatt aactgaaaaa tatagacgtc gtcccagact catgacataa
      901 agactattat tagttaactc actcactttc attgaaactt ataatgagcc ctcgaaaacc
      961 gttccattct ctcatctcac aaatattgcc ctaaatttca aactttcaaa aatgttagac
     1021 tgttgacctc gagtaaattt caaacttata ctaacaacat atataattgg aagttactgt
     1081 taggtaaagt gtcactacta aatgaaaaat tgaaacgtaa tgtaaaatga aacacattga
     1141 aaaaagaaat gatgaaaacc tcagaattct aatcgaaaat agtaaatctg cgctgagcaa
     1201 aaaaaaaaat atgactttta aaaatacaaa taattgtccg tggagtgcat tttcaaaatt
     1261 gttgccaatt aaaccaatat caaaactgtt acaaaaacta ttatttttga ggaagaaaaa
     1321 tgaagtaaat acatacactt ttaaaaagtt ttttgattcc tttagggatt gtacaattct
     1381 tcactttcaa ataatatgtt cgttttttct tactttacac aattacaacc gttacgtgat
     1441 aacgccatgt gaataaaaca ctttccaaaa ttatgaactg ccaaatttga aaaatgtata
     1501 cacaaaactg ggtgtttttg aacgttttgg tttctttttt tttgtgataa ttaaattcgg
     1561 agatcaatta ttcttgattc atgctctgct cccaactcca aagtattttt ttttgttgaa
     1621 taagtacaag aagccaagta ggtgtcagca acttgtatag ggagattatt tttttatgat
     1681 cccatccttt tttgagataa cttacctacc gattagaaca ttctttacaa taactttgta
     1741 aaaagtcatt tattgccttt tattttgtgc acaaatccaa atcctttaca aatacaccag
     1801 acaacttttt cctcccacct aattttataa gacagttttt gttatgtaat gtttcttttt
     1861 tttcccattt tctcccatta ttggtttcct cccgttaaga atctcgaatt tctacgcaca
     1921 cgtcgccgcc gcaaaaatac gtaatagggg tggcggtggc atagaatctt cctttcattt
     1981 ttcgtttctt caactttttt cctacacatc ggcggctcat tgttaaatcc attcttttga
     2041 gagggagaag atgcttgaag cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     2101 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag
     2161 aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa
     2221 aaaagaaacg taaggtcccg cgggtaagtt taaacagttc ggtactaact aaccatacat
     2281 atttaaattt tcagcgcgct gtgtcaaaag gagaagagct tattaaggag aatatgcgtt
     2341 ccaagctcta cctcgaggga tccgtcaacg gacaccaatt caagtgcacc cacgagggag
     2401 agggaaagcc atacgaggga aagcaaacca accgtatcaa ggtcgtcgag ggaggaccac
     2461 tcccattcgc cttcgacatc ctcgccaccc acttcatgta cggatccaag gtaagtttaa
     2521 acatatatat actaactaac cctgattatt taaattttca ggtcttcatc aagtacccag
     2581 ccgacctccc agactacttc aagcaatcct tcccagaggg attcacctgg gagcgtgtca
     2641 tggtcttcga ggacggagga gtcctcaccg ccacccaaga cacctccctc caagacggag
     2701 agctcatcta caacgtcaag gtccgtggag tcaacttccc agccaacgga ccagtcatgc
     2761 aaaagaagac cctcggatgg gagccatcca ccgagaccat gtacccagcc gacggaggac
     2821 tcgagggacg ttgcgacaag gtaagtttaa acatgatttt actaactaac taatctgatt
     2881 taaattttca ggccctcaag ctcgtcggag gaggacacct ccacgtcaac ttcaagacca
     2941 cctacaagtc caagaagcca gtcaagatgc caggagtcca ctacgtcgac cgtcgtctcg
     3001 agcgtatcaa ggaggccgac aacgagacct acgtcgagca atacgagcac gccgtcgccc
     3061 gttactccaa cctcggagga ggaatggacg agctctacaa gcctaggata ccggtgggtg
     3121 aagacccacc aaagccatct gccaagggag ccaagaaggc cgccaagacc gttacgaagc
     3181 caaaggacgg aaagaagaga cgtcatgccc gtaaggaatc atactccgtc tacatctacc
     3241 gtgtcctcaa gcaagttcat ccagacactg gagtttcctc caaagccatg tctatcatga
     3301 actcttttgt caacgatgtc ttcgagcgta ttgctgctga agcatcccgt cttgctcact
     3361 acaacaagcg ttccacaatc tcatcccgcg aaattcagac cgctgtccgt ctgatccttc
     3421 caggagagct tgccaagcac gccgtgtctg agggaaccaa ggccgttacc aagtacactt
     3481 ccagcaagta ggaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc
     3541 aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga
     3601 gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
     3661 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
     3721 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     3781 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     3841 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     3901 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     3961 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     4021 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     4081 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     4141 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     4201 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     4261 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     4321 ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc
     4381 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4441 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4501 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4561 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     4621 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4681 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6601 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6661 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6721 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6781 tcgtatgttg tgtggaattg tgagcggata acaatt
//
