LOCUS       sequence-441785-       10018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mgl-3 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY105
FEATURES             Location/Qualifiers
     source          1..10018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             1997..2008
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5383..5403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5425..5445
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7609..7631)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7731..7750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7828..7846)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7914..8018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8019..8087,8088..8879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8237..8256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9050..9638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9539..9558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9792..9809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9926..9947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9962..9967,9968..9985,9986..9992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10000..10016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10005..10018,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga atagtattcc
      181 cgagaacgga gattcccgta atttttctgg ttcactgaat tagttctgtt atgatgtttt
      241 ccacaatgac acaaaaaata gtaagtgaaa tattcatatt tctataaaat ccaaactttc
      301 cgttgaactt tattgaaatt ttttggtttt gttttaaagg aagtgtttga tacttttact
      361 gttttattga gttactgttt tattgagagc aaattctgaa tattcgtgga aaaaagaatt
      421 ttaataaact aacaggcata attttaatat ttttggtgaa atagtaaaaa atcagcgtct
      481 gaaagatgca gaacttactt gaatattgaa aattttcaat tataaaattg taatctgctt
      541 cataatgttt ttaaaaatta gggaattcct ttggatatgt tacaaggact gctagttttg
      601 ataagattta atgtgaaata tttcttcatg gaaattaaaa tttgaacaat aatgagattc
      661 gctaggaaaa tttccaataa attttttgac aatatgttaa aaatttattg cacaataatt
      721 ttgataattt tttgcaaata accgaaaaaa aaaatagaat acgtagattt tgccacaatt
      781 atcaaaacaa tatttgctcg aatgaatatt catttttgat aatccagaga cagacaaaat
      841 ataactatac tttttctgga aacatgaaaa attattagaa atgcccaggg attaatttga
      901 tttttgaaaa attgtaactt gtttcataat gctttcataa gtttggattt gcgtttggct
      961 cattacttgt aatgacattt caagattata tgaaaattac cttcacagca gaaaacaaca
     1021 ttggattcag ataagaaatg caaaaatcgt gataaagttc caaaatattc tttaaacaat
     1081 ttttcaatca tactggcaat cattagagat accaaaattt ttctggaaat cttaaaacaa
     1141 aatcatgaaa aaataatcat ttaaaaataa tatgattttt caaaatcctt acctgtttct
     1201 tgatgttttc atcaactctc tgaacgcttt tatttattag ccagccgtga cggcaccttc
     1261 ggcgcggccc ccgacttctg gggctgaaaa ttaatttttt atgaaactac cgtaacccta
     1321 cagtattcct accgtaccac tattgtacca ctacagtacc ccgactatat cccttcacta
     1381 accccaactc actatccctc cagaagccaa aacttcacag actacaaaga ctacatagac
     1441 tacaaactat ggacacacag aataagcgct ttatatatag taaattgaat tttcgattga
     1501 atcgattgaa ttttttacct tcaccttttt ctctctaatt ttcttccacg tcttcttgaa
     1561 agcttaaata aataaaatta aatagacaaa ttatctactt tttaaaccag gtgggataat
     1621 ttttcaataa tatgaattat caataaattg ttaactggtt gtaattttaa agtaagtcca
     1681 tattttcagc tctaatgaaa agtaaaggct ttccaaaaat acaaaaatta agatacagtt
     1741 tcatgagatg tacaaaaaat ttcaagcata gccataacca attctaggac atttttgcag
     1801 aattaatact tttgcaggaa atcttaataa aatcatgtat tcatataaaa agtcactgcc
     1861 aaatctatcc gacccaatat cccattacca cgtactaatc ttcctcgaat cctctcatca
     1921 ttttccgatt tgattttttt ctttttttct tcaacagaaa tagcagattc aggaggaaaa
     1981 gtcatttttc ttagtttttc tcctctttaa atactaatct tccattccga aaataaagag
     2041 agttaagcga tgagagagcg tgaatgagtg agacacagac aaacacgtat ttgatatttt
     2101 ttggcctaat ctccctccca tctctttgct cctgctccgc caacttgtcg attcagatta
     2161 gcaatttgtc aagccctgtt tgtgtggctc aattgattcg tttttttttc tttttttttg
     2221 gcatttctgc tgaatcttct aatttcctat atgatcaaat aacgtagaca ctataattgt
     2281 aagccacctg tttttttttc gtatttgtta tcaatttgaa atgggcaaaa tatcttaatc
     2341 acaagttttt taatgccaaa aaaacttttt ttgtcataga aaatatgagt cactgtttcg
     2401 agtatgttat aaagatacgg tatataattg ttaaataatg cactgggata tttatatgta
     2461 ttactgtcat acaattagaa aagtttccta acaaactcga tgcataaaca agcagtagtc
     2521 aatttttgga tggggcctta atgtttttat tcattgaatc cattggttcg tttaaatcaa
     2581 agcttcgcaa aaattaggta tttctatttc taggcgttcg caaaaaattt attcgtactt
     2641 ttgtactctt tgttgtagtg actgataatg tttctgacaa aaatatatca ttaacagcca
     2701 aaacatagag tacggtcagt tcagccattc aatgaaatga aatataatta tagttttaaa
     2761 aatccaacga gaaacgaaac tgtaagagat ttctttttta attacttttg gaagtttttg
     2821 ctctagaaat catagtaata tgggttaagg ctttactgaa atttcaagtt tcgattttat
     2881 tttttttgaa aatttatagt aatacagacc gtaccatagt tagtacggac agaagggaat
     2941 ccctcttgaa tgtaataacc caattgagaa acgcatgtgg tgagcttgat tgattcttac
     3001 ggacctggtt tgatgtcggc tgctgatttt aaaagttcct gtatctaggg aactattgtt
     3061 taaaacattg ggtagaaatt aattttccca aacttccatt tttcgatatc aacattgcaa
     3121 gatgaacaga aatacttttt cggcttactc ttcatttcct agttttcata ttattagaat
     3181 caagattaag atcaattttt cactaatccg cgtacacttc atcacaatat tattttatag
     3241 tgaagactcc cgtatcagta attttttatt gggggtattc tgttttcttt gggaacaatt
     3301 taaaatccga ctgactgagg aacagatcag ataaaatagt gaattgacgt atatctgaaa
     3361 tttacgaaaa agaggatcag ataaagtaga tcatctgggt cagtgaatca tttgaatttt
     3421 agttttttga tttcggattt gagaaaaaat aaaataaact gtttcaatat ctctataaaa
     3481 ttaggcaaaa tattgggatt ctacttcact tttttaaaaa cattttcatc aactttgcat
     3541 ctatttttca aactaaactt gcttcatttg tattacgaaa ctttattatg tacaaaaatg
     3601 cctgcatgat gcctattcgc tcgcctacta tcaaaattct taaaaaccaa aattcgtatt
     3661 atgtggttag ttgaaccttt tcaaaaaaca attgaaattt ttaagcttat agtttcgtaa
     3721 atctacattg catggagata cctagtttcc ataatgccga aaataggcaa caggtaggca
     3781 cgtgggtagg cagacaaagg catgcctggc aacaaaaaat accgattatc cgttacctac
     3841 ttttaatcgc tttttaaaat cgtttccata taattttccc accccttttg ttcaggtttt
     3901 ctcttttttt tcttcatata tacatttttt ctaataattg ttttgttttg gcgtttcccc
     3961 aaaaaagaaa atattctctg aaaagtcaat gaagccccct tttttccctt ctaatctctt
     4021 tagttaccac cttctctcat gaatatttag aatgagatct atgttgtcat caaccaccca
     4081 aaaatcttcg acaccgttta cacccccata tatatacata tttatctagt tacatacata
     4141 cgtactcaca cacacacaca aggcaaaaga aaaagacctt ctaaataatt cgtcttaaat
     4201 aatttatgtt aatctcaatt ccgattcgca gctcactagc cgccatgttt actttccttt
     4261 cagactcttt ctcacttttt tccagttcag aaatcttctt cgtctactga tattattttt
     4321 gctaattaat ctagtattaa tttgaaaact attcaataat tcctgacaat ttaaaatatg
     4381 cattcgttga aacggttttt ttttgaaaag ttactataat atattatcaa gaaaatggca
     4441 tgcataaggt tctgttaaaa caaatcaaac tatttttgtt tataagaaaa acatctgaaa
     4501 gtcaaaaaat gtgttttacc agtttcaaca aaaaagaaaa agctttccta taccaaattt
     4561 taaatatgta ctctttccat caattactga gaaaaacaat tctaacatat ctattaagtt
     4621 gcagatttag taggaattat tatttattta aaacaactac tttttaaacg aaaacataac
     4681 ttccaactgt ttctgatagt taataaaagc ttcaattctt ctttaaacgt ggtgtagacg
     4741 tattttttta gtgctttatt aggtccaaaa ttgtctgaaa aaaagagtta tggcggctca
     4801 aaaaatgatt ttacccaatt ttaccaaatc ttgacgtcca tcgaccttaa atgtaccgtg
     4861 taagttttca aagcttttga aacagtaaaa tgttaaacat tccaactatt tctgatagtt
     4921 cttgaaaatt cccattttat ttgttgattt agtggtgcac cgtcttgctg aaaacactca
     4981 ataaattttt aattttaata gtatttcggg tttgctgaaa ttatatcttc cttctaaaaa
     5041 cttttcactg tttcacgttg taattttaag atgaataacc aaataggcag ggattgttct
     5101 ttattactgg gcgtcatacc ttggcatttt atttcttata catacgtgaa tcttatgcta
     5161 aacttgaaaa ttattctgaa aaaaaaaatc aaaattccag ataaataatc ccaaaaaccg
     5221 tttcaaaacg gagttccatc aagaaatcgc gtcatcgctt gtccggatcc cgggattggc
     5281 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     5341 acccttgctt ggagggtacc gagctcagaa aaaatgaccg ctccaaagaa gaagcgcaag
     5401 gttccagtcg agaagatgac tgccccaaaa aagaaacgta aggtcccgcg gatgtcagag
     5461 cttattaagg agaatatgca tatgaaactg tacatggaag gaaccgtgga caaccatcac
     5521 tttaagtgca cttcggaggg ggaaggcaaa ccgtacgagg gcacccagac catgcgaatc
     5581 aaggtggtcg agggaggccc actgcccttc gccttcgaca ttctggtaag tttaaacata
     5641 tatatactaa ctaaccctga ttatttaaat tttcaggcta ctagcttcct gtacggcagc
     5701 aagactttta ttaatcacac ccagggcatc ccggacttct tcaaacagtc gttccctgag
     5761 ggcttcacat gggagcgtgt gaccacatac gaagacggtg gcgttctcac cgctacccag
     5821 gacaccagcc tccaggacgg ttgcctcata tataatgtgg taagtttaaa cagttcggta
     5881 ctaactaacc atacatattt aaattttcag aagatccggg gagtaaactt cacatccaat
     5941 ggtccggtta tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtatccg
     6001 gctgacggag gcctggaagg tcgaaacgac atggccctga agctcgtcgg cggtagccat
     6061 ctcatcgcaa acgctaagac cacatataga tccaagaaac cggtaagttt aaacatgatt
     6121 ttactaacta actaatctga tttaaatttt caggctaaga accttaaaat gccgggagtc
     6181 tactatgttg actacagact ggaacggatc aaggaggcca acaacgagac ctacgtcgag
     6241 cagcacgagg ttgcagttgc cagatactgc gacctcccga gcaaactggg tcacaagctt
     6301 aatcctagga taccggtggg tgaagaccca ccaaagccat ctgccaaggg agccaagaag
     6361 gccgccaaga ccgttacgaa gccaaaggac ggaaagaaga gacgtcatgc ccgtaaggaa
     6421 tcatactccg tctacatcta ccgtgtcctc aagcaagttc atccagacac tggagtttcc
     6481 tccaaagcca tgtctatcat gaactctttt gtcaacgatg tcttcgagcg tattgctgct
     6541 gaagcatccc gtcttgctca ctacaacaag cgttccacaa tctcatcccg cgaaattcag
     6601 accgctgtcc gtctgatcct tccaggagag cttgccaagc acgccgtgtc tgagggaacc
     6661 aaggccgtta ccaagtacac ttccagcaag taggaattcc aactgagcgc cggtcgctac
     6721 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     6781 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     6841 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     6901 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     6961 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     7021 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     7081 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     7141 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     7201 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     7261 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     7321 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     7381 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     7441 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     7501 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
     7561 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7621 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7681 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     7741 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7801 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     7861 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
