LOCUS       sequence-441789-        6289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lin11(intron 7) fluorescent neural reporter driving nuclear mTagBFP2
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY103
FEATURES             Location/Qualifiers
     source          1..6289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1654..1674
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1696..1716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3880..3902)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4002..4021
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4099..4117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4185..4289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4290..4358,4359..5150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4508..4527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5321..5909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5810..5829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6063..6080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6197..6218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6233..6238,6239..6256,6257..6263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6271..6287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6276..6289,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga tccgtgaaca
      181 acttgccgcc gagacaggac tcaacatgag agtcattcag gtttctattc ttcttctttt
      241 tttctttttt tgcacatgtc taagtatcct cgaaatgcac tatctctcac aaattctcat
      301 ttcccgcttt aattcacctg ccccgccctt ctctctcttg ggggaggaca catccttcca
      361 cacgcacaca cacaaattca attaccgtag tccttgaaag gagagagagg gaaaaacgga
      421 aagaagaaaa agagaaaaaa gtgagtggat actgtagttt ctaactaata tcatgttatt
      481 tactgtaaga aagatgtaca catcacatga tttttatgta acaatagaaa taataatgat
      541 cataatcata ataactgata acaggaaact taacggaaag ccataggaaa ttggttgtgt
      601 ttagagagct atacgaaaat gatgatgaaa gagaaaatgg gaaacaattt caatttggaa
      661 gataaaaatc tgagtggtca agggagagaa ttgcaatgag aaatttgagt tgaactcaga
      721 aaattcgttt ttttgttcaa atttttgaac cctgaatatt cggaattcca aaattaattg
      781 tagtcttaat tttttgtgtg aaatattttt agcctacttc caccgatcaa ttaaatttta
      841 attctgttta tctgcacagt tgccccataa tagtaaactt aaaaaactgc taacacataa
      901 cttttagtgt tttgattcga aaatttggaa ataactgaac taaaaaaggg ttcgctctct
      961 atattgattt ttttcaaaaa tttaatcttc gtgtacagtg ttttagttca cttttaaact
     1021 tctaaatgaa aaagtagtca actacagagg accattcgga actgaaaaaa ctcgatctga
     1081 atagaacttt ttttcataaa aacatatgat tgaaaaaaaa attcgactat tttttctttt
     1141 ttttcttgga tttctggact tgggaaaata atccgggcgt tttcgaaaat ttttcaatcg
     1201 ttcaaaacaa tctgaaattt taaaaattga atttgggtga taaaagattc agtaattttt
     1261 ttcaaaaaat tcaaagtttc caaaatcttt aagaatcttc cataaatcta cttttggtaa
     1321 aattccgaga atgaaaatga acaatttcag atcggctttt tcaagttcaa aatgctcttc
     1381 tacagataac ataatttttc tgtggggctc actcaaaata tatttttcca aatgacacag
     1441 ggtttacctt taatagataa ttttctccat taatttgttt tccaaataaa tttccatttt
     1501 acaggtgtgg tttcaaaacc gccgaagcaa ggaacgaagc ccgggattgg ccaaaggacc
     1561 caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgct
     1621 tggagggtac cgagctcaga aaaaatgacc gctccaaaga agaagcgcaa ggttccagtc
     1681 gagaagatga ctgccccaaa aaagaaacgt aaggtcccgc ggatgtcaga gcttattaag
     1741 gagaatatgc atatgaaact gtacatggaa ggaaccgtgg acaaccatca ctttaagtgc
     1801 acttcggagg gggaaggcaa accgtacgag ggcacccaga ccatgcgaat caaggtggtc
     1861 gagggaggcc cactgccctt cgccttcgac attctggtaa gtttaaacat atatatacta
     1921 actaaccctg attatttaaa ttttcaggct actagcttcc tgtacggcag caagactttt
     1981 attaatcaca cccagggcat cccggacttc ttcaaacagt cgttccctga gggcttcaca
     2041 tgggagcgtg tgaccacata cgaagacggt ggcgttctca ccgctaccca ggacaccagc
     2101 ctccaggacg gttgcctcat atataatgtg gtaagtttaa acagttcggt actaactaac
     2161 catacatatt taaattttca gaagatccgg ggagtaaact tcacatccaa tggtccggtt
     2221 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtatcc ggctgacgga
     2281 ggcctggaag gtcgaaacga catggccctg aagctcgtcg gcggtagcca tctcatcgca
     2341 aacgctaaga ccacatatag atccaagaaa ccggtaagtt taaacatgat tttactaact
     2401 aactaatctg atttaaattt tcaggctaag aaccttaaaa tgccgggagt ctactatgtt
     2461 gactacagac tggaacggat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     2521 gttgcagttg ccagatactg cgacctcccg agcaaactgg gtcacaagct taatcctagg
     2581 ataccggtgg gtgaagaccc accaaagcca tctgccaagg gagccaagaa ggccgccaag
     2641 accgttacga agccaaagga cggaaagaag agacgtcatg cccgtaagga atcatactcc
     2701 gtctacatct accgtgtcct caagcaagtt catccagaca ctggagtttc ctccaaagcc
     2761 atgtctatca tgaactcttt tgtcaacgat gtcttcgagc gtattgctgc tgaagcatcc
     2821 cgtcttgctc actacaacaa gcgttccaca atctcatccc gcgaaattca gaccgctgtc
     2881 cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt ctgagggaac caaggccgtt
     2941 accaagtaca cttccagcaa gtaggaattc caactgagcg ccggtcgcta ccattaccaa
     3001 cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc
     3061 tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta
     3121 agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     3181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     3241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     3301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     3361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     3421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt
     3481 cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat
     3541 ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt
     3601 ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca
     3661 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     3721 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     3781 atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg
     3841 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     3901 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     3961 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4021 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4081 catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4141 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4261 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4321 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4381 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4441 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4501 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4561 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4621 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4681 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4741 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4801 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4861 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4921 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4981 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5041 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5101 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5941 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6001 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6061 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6181 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6241 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
