LOCUS       sequence-441790-        5982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  odr-10 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY110
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1347..1367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1389..1409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3573..3595)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3695..3714
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3792..3810)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3878..3982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3983..4051,4052..4843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4201..4220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5014..5602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5503..5522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5756..5773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5890..5911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5926..5931,5932..5949,5950..5956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5964..5980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5969..5982,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt caaagtgctc
      181 ctctgccgct acattttttg gaggggccta cttaaagaag gagtatcgtc aatgggaaaa
      241 ttgttttaaa atgtagtatt tgtactcaaa ctttcaatta tcgcaaaaga aaaacggaaa
      301 aaatccgtta acattcagca tttagccgtt gaaatcgacg aaagtggctg aaagtggctg
      361 aatttgaatt tcgcgcgcaa atgaatccta gtccaccacg caacttttag gccatattcg
      421 taaacaaaat attgatttaa atgtaatttt tcgaacttta attcgctttt ctttctggaa
      481 aagttcaaga aaactgggaa gaagaaattg tattgagtat tttttcaaag gacaatacca
      541 aaacaaataa aaattcgaaa gatcgtcgtg gcctaggatt tctgtagtta atttttaaag
      601 attttttcga cttaaaatgc tgaatctaag cggattctcc cgtttttgtt ttgtaatatt
      661 cttaagtttg agcacgaata ctacatttta aaacaatttt cccattgacg atactccttc
      721 tttaaagggt cttcttcaaa atggcatagg tttcattcag aagtccggga ttatgttgaa
      781 atgcttccct tgtgagtagg cagtaaatac atccaatttc acataatcga agagagatca
      841 ttcatttagg atacattgtg ttgtgattca tgacatttac tacaacacaa ccagctgatg
      901 tgtgtgattc aggttttaat tgtagcatct tgtgattgga ataataaatt caatatattt
      961 tcccaattct gaatttgtgg atacaaacgt attattttta actgaatacc tcgtgaaatc
     1021 agatttcaga ttttgaaaac aatatatctg actataagtt gcgtatgtag acaaattcca
     1081 ggaaaataat ggctcaaacg acacgcccct ggcttcaagt tactataaaa taggttagga
     1141 acaaagcaca tataaccatg tattcagttt aattaatttt ttgttggcgg tggcatgaaa
     1201 ttctcataat ttcattaaga taccttacag ctcccgggat tggccaaagg acccaaaggt
     1261 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt gcttggaggg
     1321 taccgagctc agaaaaaatg accgctccaa agaagaagcg caaggttcca gtcgagaaga
     1381 tgactgcccc aaaaaagaaa cgtaaggtcc cgcggatgtc agagcttatt aaggagaata
     1441 tgcatatgaa actgtacatg gaaggaaccg tggacaacca tcactttaag tgcacttcgg
     1501 agggggaagg caaaccgtac gagggcaccc agaccatgcg aatcaaggtg gtcgagggag
     1561 gcccactgcc cttcgccttc gacattctgg taagtttaaa catatatata ctaactaacc
     1621 ctgattattt aaattttcag gctactagct tcctgtacgg cagcaagact tttattaatc
     1681 acacccaggg catcccggac ttcttcaaac agtcgttccc tgagggcttc acatgggagc
     1741 gtgtgaccac atacgaagac ggtggcgttc tcaccgctac ccaggacacc agcctccagg
     1801 acggttgcct catatataat gtggtaagtt taaacagttc ggtactaact aaccatacat
     1861 atttaaattt tcagaagatc cggggagtaa acttcacatc caatggtccg gttatgcaga
     1921 agaaaacact cggctgggag gccttcaccg agacgctgta tccggctgac ggaggcctgg
     1981 aaggtcgaaa cgacatggcc ctgaagctcg tcggcggtag ccatctcatc gcaaacgcta
     2041 agaccacata tagatccaag aaaccggtaa gtttaaacat gattttacta actaactaat
     2101 ctgatttaaa ttttcaggct aagaacctta aaatgccggg agtctactat gttgactaca
     2161 gactggaacg gatcaaggag gccaacaacg agacctacgt cgagcagcac gaggttgcag
     2221 ttgccagata ctgcgacctc ccgagcaaac tgggtcacaa gcttaatcct aggataccgg
     2281 tgggtgaaga cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta
     2341 cgaagccaaa ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca
     2401 tctaccgtgt cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta
     2461 tcatgaactc ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg
     2521 ctcactacaa caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga
     2581 tccttccagg agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt
     2641 acacttccag caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct
     2701 ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac
     2761 taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa
     2821 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     2881 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     2941 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     3001 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     3061 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     3121 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     3181 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     3241 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     3301 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     3361 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     3421 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     3481 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     3541 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3601 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3661 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     3721 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3781 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3841 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3901 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3961 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4021 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4081 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4141 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4201 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4261 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4321 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4381 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4441 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4501 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4561 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4621 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4681 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4741 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4801 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5641 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5701 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5761 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5821 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     5881 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     5941 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tt
//
