LOCUS       sequence-441791-        7559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  odr-2 (promB) fluorescent neural reporter driving nuclear CyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY111
FEATURES             Location/Qualifiers
     source          1..7559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2918..2938
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2960..2980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5150..5172)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5272..5291
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5369..5387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5455..5559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5560..5628,5629..6420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5778..5797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6591..7179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7080..7099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7333..7350
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7467..7488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7503..7508,7509..7526,7527..7533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7541..7557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7546..7559,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tagtcagcat
      181 ttcaccctga tttcttgctc atttttaccc aaaaaatata attccaaata aaaaaaaata
      241 ttaaaaatta tctcacatac tacacaaaga aattgtctga aatgtttgtc ttgattatct
      301 cacatactac atttttatat ctaattctca tccaaatctt taaaaatgtg ttatggatgg
      361 ttccagtttt gcctttaacg tctcgacatg aaaacaactt ttggataatt ttattacaga
      421 taatcagaaa aatgaaatca attgcaacca aaaaactgca aaaaatctat cgatttaaat
      481 gagaatttca agttaacggt tctagttatt tctacagccg tgcattccat agaaggcgcc
      541 cttgagcttg ttttctaatt tatttatttc catgtgtgtt cctattcgtt tgtgacaatt
      601 ggtttagcaa ccaacaccac gtatttctgc cattagagag cacctttttg ccacctttcg
      661 actcccttac tctctttgta ttaaatggcc tttacctctt gtctagttgt tgctcctttt
      721 atttgtccat tgctccagcc gcttttcatt cgaggtcatg catttctgtg gtgctccatt
      781 ttctattttt gcttcttcgt tttcgtcttc tttccttttg tggcttcaac ctcaattatt
      841 ggaacctaag gtgtggtaaa gaatgatgga aaactcgaaa aagtttgtaa gttttagtag
      901 tcgccgtgac ttcacaccct agatgctccc ttctttttct tccatttaat tcaaacaaga
      961 gtcatgaaat aaatttaatt aaatgattga aaaactgaga taagcaagtg ccatttctct
     1021 tgttgaccct cttttattca ctactattgt tttgagttgt tgctatttac agcttttgct
     1081 aagccttttg tttgcaacct ttttacctcc cgtttttagg tagatttgaa attgttttca
     1141 cgagtaggaa gaattacgca gaaatgttat atacgagtgt tgaaaattaa cgaacattag
     1201 aagtctaatg gcagaataag ctcaaaaata ttacaatgga atgatcgcaa aaaatattaa
     1261 tccaaaatga tgtttcaatt ttatcgtgct catgaccaca ttagtgaaga aacaaaagtt
     1321 ctgcaagttc cagaataaat ttttcgaaca tcattgcttt tggtggcaac tttttacaca
     1381 agcttaatcc aagcttaatc caagtttaac gtaacttttg gcaaatttct taccaaagtc
     1441 tagttcgact tttgcccaag ctttccaaac ttttgaaata catgtttttg ggtccaactt
     1501 gggagccaaa tgcagagatt tcaaattagt taatgttttg atacatttac aaaatagttt
     1561 tctctacaac tgaaacgcct ggagatggat ttggaggttc tagtcatttt tgaaaaattc
     1621 aatacaattt ctacacaaat caaaatttca cgatcgccga aacgttagta acaaatattg
     1681 ccggcttcac aaactgctga tttggttggt taggcaattt ctgtaatttc ggtgtaattg
     1741 ctaacaaaaa tattctcact ttagtcaatt tccacccggc aatctatatt catcccttaa
     1801 aaagttactt ttgctccaat atgttgaata tttcaatctc cggatgcagt ctcaccttgt
     1861 ctttagaaga attcggaaat tggtttaacc ttttttatcc cgtcacgaga tactatttcc
     1921 tgtcactcgg aaccagaccg accctattcg tcaacttttt tctttgtact aattgtcacc
     1981 tttttcattt tgtactgttc caattatgtg tttgctcaaa aagaatactt ctccgggata
     2041 aatgggggat gcgccttgtc gtcgtttact tacccgtgat tctttcttcg ttttccgacg
     2101 cctcccgcaa ttactatcac atattctatc cgacgatgtg cccctaatcg tgttactcac
     2161 actctttcac tccttctttt agttgtcttt acttgacttt agattttcta aagaattatt
     2221 ttgagccatt tttagatatt tttattcgat gatatttttg taagtatgta tcacttgaga
     2281 tatacttttt acaacaaatt tcaaaataac aagtatttta ttacactaca agcaactttt
     2341 cagaagtcat caaaatatgc tccagaacta aattgaaatt taagattgca tcatcttttt
     2401 cagaaaattt tcagactcca gccgttcaaa taatccttgt aaaatctaaa gtttttccaa
     2461 gtttttgggt gtactatctg ctcaattcaa acattgcgga gcatgcatca ataatttgcg
     2521 tttttcatgt ttcagaccat taccattaaa ctattgtttt agctttaata tttctcaaac
     2581 gttttgcttt aatatctgtt gtaccgtact caatgaattt ctatttataa agctttatta
     2641 tttactttca gattcatccc ttggtatttg aaaatttgca aagacattca gcaatattct
     2701 caaaatctct tctgtctcgc tattttcacg tgtcccaaaa ttccccaatt aagttggtca
     2761 acttccaaaa tttacattcg gaacatttat atttcagaca gaacccggga ttggccaaag
     2821 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
     2881 tgcttggagg gtaccgagct cagaaaaaat gaccgctcca aagaagaagc gcaaggttcc
     2941 agtcgagaag atgactgccc caaaaaagaa acgtaaggtc ccgcgggtaa gtttaaacag
     3001 ttcggtacta actaaccata catatttaaa ttttcagcgc gctgtgtcaa aaggagaaga
     3061 gcttattaag gagaatatgc gttccaagct ctacctcgag ggatccgtca acggacacca
     3121 attcaagtgc acccacgagg gagagggaaa gccatacgag ggaaagcaaa ccaaccgtat
     3181 caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca cccacttcat
     3241 gtacggatcc aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     3301 tcaggtcttc atcaagtacc cagccgacct cccagactac ttcaagcaat ccttcccaga
     3361 gggattcacc tgggagcgtg tcatggtctt cgaggacgga ggagtcctca ccgccaccca
     3421 agacacctcc ctccaagacg gagagctcat ctacaacgtc aaggtccgtg gagtcaactt
     3481 cccagccaac ggaccagtca tgcaaaagaa gaccctcgga tgggagccat ccaccgagac
     3541 catgtaccca gccgacggag gactcgaggg acgttgcgac aaggtaagtt taaacatgat
     3601 tttactaact aactaatctg atttaaattt tcaggccctc aagctcgtcg gaggaggaca
     3661 cctccacgtc aacttcaaga ccacctacaa gtccaagaag ccagtcaaga tgccaggagt
     3721 ccactacgtc gaccgtcgtc tcgagcgtat caaggaggcc gacaacgaga cctacgtcga
     3781 gcaatacgag cacgccgtcg cccgttactc caacctcgga ggaggaatgg acgagctcta
     3841 caagcctagg ataccggtgg gtgaagaccc accaaagcca tctgccaagg gagccaagaa
     3901 ggccgccaag accgttacga agccaaagga cggaaagaag agacgtcatg cccgtaagga
     3961 atcatactcc gtctacatct accgtgtcct caagcaagtt catccagaca ctggagtttc
     4021 ctccaaagcc atgtctatca tgaactcttt tgtcaacgat gtcttcgagc gtattgctgc
     4081 tgaagcatcc cgtcttgctc actacaacaa gcgttccaca atctcatccc gcgaaattca
     4141 gaccgctgtc cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt ctgagggaac
     4201 caaggccgtt accaagtaca cttccagcaa gtaggaattc caactgagcg ccggtcgcta
     4261 ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta
     4321 aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct
     4381 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     4441 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     4501 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     4561 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     4621 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     4681 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     4741 tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt
     4801 tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct
     4861 tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc
     4921 taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga
     4981 ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt
     5041 tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc
     5101 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5161 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5221 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5281 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     5341 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     5401 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5461 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5521 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5581 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5641 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5701 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5761 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5821 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5881 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5941 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6001 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6061 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6121 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6181 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6241 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6301 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6361 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6421 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6481 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7261 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7321 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7381 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7441 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7501 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
