LOCUS       sequence-441792-        6838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ocr-2 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY109
FEATURES             Location/Qualifiers
     source          1..6838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2513..2533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2558..2578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2600..2620
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4429..4451)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4551..4570
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4648..4666)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4734..4838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4839..4907,4908..5699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5057..5076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5870..6458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6359..6378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6746..6767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6782..6787,6788..6805,6806..6812)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6820..6836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6825..6838,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcggatcga ccattcattt
      181 ctgcagaact tatattgtgc gtgactatgt gtttacaacg acatctcgct aggcatacgg
      241 aggcaaggaa gcgcagagat aaaagacgaa caacagaagg tcaagaatga gttagacatg
      301 aagagataca gatagttgga aaaacaagaa aaaagatgca aaatacctga taattgctgt
      361 tttcgaaaca attaaaactt tttgatgttg tttgatgtgc cacgtgacaa atgaatgaag
      421 ttacagagca ttgataaact aacatttata agttgatgaa gtgatcatgg aacaaaatta
      481 ctacgttttt tttcagttta tcctgaatcg ggagacattt tgcaaccaat agttccttcg
      541 aaaaattggt ttttatcata attcagattt acacggaaat gttccatgaa aagatagcat
      601 ttttaaaaga ttttcagatt aaaaatggag tagagctagt ggagacttta tataactgca
      661 cttataatac ttgaacatga tcaaatataa taaatcaaca ctcggaaagt ttaaaaaaga
      721 atttttgttt caattcagta aaatgtttgg aaaattggca aactatcaaa acaatttggt
      781 ctttttgagt aattccgatc attgttgcaa gtttaaacct cgacgatgtt caaacacaag
      841 tagttagaat tttttaaaca aatttaaaaa aaggtttcga aattaattac tttttttttg
      901 aatttgaggt tccaagattt cacttacata agctcaaact ttgtgtaggc acttttaaaa
      961 attgtcaatt agaaacttgt atcaagataa tattcaaatt ctataatttt caaaaaaatc
     1021 ttcaaaacat taattttctg tggctctagt aacaaagata aaagggtgca acactaattg
     1081 aaactgaaat actttaaggt tcaactttgt attttaatta cgtttgaatc agttgtattt
     1141 gttttctaga tatttaaatc tgcagcaatt cttgaaagtt ctcattttaa taaaattctg
     1201 ggaatttacc aaaactttgg ctgaaatttt taaattcgaa ataaaattga aacattttgt
     1261 atcatctttg tttatttatg ctcttttaaa atcgaaaaaa aatcggagct tttatttatg
     1321 atctcaagat gtgttggact attagtaatg tatttagaaa gtattaattt tgatagttcc
     1381 gaattgttct tgtcataaat atacaaagat ggtacaaaat gtttcaatat cgattgtttg
     1441 aaaatctaaa actatcttca catgtttaat tcattctttc tccccataaa atcactcttt
     1501 taagatctat caagttttca gaagtttctt gtacaaactc ttttcacatt ttgcctcctg
     1561 atatccaatc tcccacggca acaaaccgac cacgacgacc gacacgccaa aattcaatca
     1621 aaaagcaggc gcctgttcaa ccaattttct ttcagtgtca tattctacct tctctctatt
     1681 tctttcaatg ctttctcttt tccttttccc cttcatggag attcaggagt gagcttgagt
     1741 ataacagcca cgcccatttt ttcgccttct ccatccatct tatagctccg gctcaagagt
     1801 gagccacgaa attcagtttt gttattctga ttttggtttt ttttgccatt cacaaaaata
     1861 caatttcaaa atccatcacg ttattagaca aatgaacttg gggtcgccaa aaatgtcagc
     1921 tgctttaaaa gatagatgac aggaacttgg gatcaaaacc agatttcata taactggcaa
     1981 tattttaaga acctttcatt aatttagtat atcaattcat aaagatattg gtttaagttt
     2041 tggttcaaaa ttgaaccaaa attgatcata catacatttc cctgccccac attatcacat
     2101 gtagatgcct acctcttacc tgcctaccta tatacagacc tgctctcagt tttctgcatt
     2161 ccttgaatgc aagtcagact taaaggcatt cattgaaagc attgcctaga aagttgagat
     2221 ttttgggttg gcctggaagt cttaaaattc aatgatactt atatcacaaa atcattaatt
     2281 ttctaacttt ggcttttttt caattctatc atttaattta attttcagac atatcttaat
     2341 catcttatca gtagagtaca tcacatcatt aagacccggg attggccaaa ggacccaaag
     2401 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggagggta
     2461 ccggttttcc cagtcacgac gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa
     2521 gaaacgcaaa gtaccgagct cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc
     2581 ggtcgccgag aagacagccc caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag
     2641 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtctccaa
     2701 gggagaggag ctcatcaagg agaacatgca caccaagctc tacatggagg gaaccgtcaa
     2761 caaccaccac ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcacccagac
     2821 caacaggatt aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac
     2881 ctgctttatg tacgggagca agaccttcat caaccacacc caaggaatcc cagacttctt
     2941 caagcaatcc ttcgtaagtt taaacatgat tttactaact aactaatctg atttaaattt
     3001 tcagcctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac
     3061 cgttacccag gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg
     3121 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag
     3181 taccgagacc ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa
     3241 gctcgtgggc gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc
     3301 cgctaagaac ctcaagatgc caggagtaag tttaaacata tatatactaa ctaaccctga
     3361 ttatttaaat tttcaggtct actttgtgga ccgcagactg gaaagaatca aggaggccga
     3421 caatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
     3481 caaactgggg cacaaactta atggcatgga cgagctgtac aagtaagcgc cggtcgctac
     3541 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     3601 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     3661 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     3721 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     3781 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     3841 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     3901 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     3961 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     4021 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     4081 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     4141 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     4201 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     4261 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     4321 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
     4381 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4441 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4501 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     4561 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     4621 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     4681 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6601 cgaccgagcg cagcgagtca gtgagcgatg aagcggaaga gcgcccaata cgcaaaccgc
     6661 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6721 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6781 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
