LOCUS       sequence-441794-        6452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nsy-5 (prom4) fluorescent neural reporter driving nuclear CyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY108
FEATURES             Location/Qualifiers
     source          1..6452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1811..1831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1853..1873
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4043..4065)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4165..4184
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4262..4280)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4348..4452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4453..4521,4522..5313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4671..4690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5484..6072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5973..5992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6226..6243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6360..6381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6396..6401,6402..6419,6420..6426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6434..6450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6439..6452,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aatttaagct
      181 gaaattccga aaaattacgt aattttccat tttttccccc aaattttctc ctttttttta
      241 ctgaaaaagt ctacttttca aacaaaaatc ctcaaatttg caaaaaaaaa caaataaaaa
      301 aaattttcat gaaaaaaatc ggtaaaattg tgattttttg aagcaaaatt ttcagataaa
      361 aaattcagaa aattaagtaa attcccagtt ttccccacat tttccccttt ttcgctgaaa
      421 ttttttcccc caaaaaattc ttccctctct tcaaggttgt gtggttcatt taatactgaa
      481 ttgtattgaa ttcaatcaac gtctcgtttc atgtaagctc tccttcctac cttttcgaga
      541 taaacttttt atgatttgta ttatgtaaag cagtcataaa tcccacggat tgggtggcag
      601 cgggggatat ttaattaaga aaagtgcaga aggtaaacgg agacgaggct ttctgttgtt
      661 attcaacact ttcggattta cgatgtcctt cggcttcttc cgttgagctc cgttgagctc
      721 ccgagtaaac gtagtagcga aattcgattt acggattagg ttgcaccttt tatgaaatga
      781 aattgtttgg agtcatccga tgatgatacc tttttccatt tttatttctt tttttttaga
      841 aaaaaaacgg agaaaaacca cgtgtttctg gattttttca atgctgaaat aatcataaaa
      901 ttgaattttt tacagttttc gctgaaaatc agttctaaat agagtatttg tcgttgaaaa
      961 ataagaattt aattactttt ttcgggggtt tccatcaatt tcgactcaat ggggtgccaa
     1021 aatctgggaa atattttaaa attacttcaa attttcccct gattcagaat atttgtgtga
     1081 aaaattgaaa aaaaaatccc gtaaaccaaa aatctattac gggaactata ttctgagact
     1141 gcgtactatt aatttaagaa gtcaaaaagc ggattagcgg aattattacg ggaacacaaa
     1201 attgtgagaa tgcgtactgc acaacataat tgacgcgcaa aatatcttgt agcgaaaact
     1261 acagtaattc tttaaatgac tactgtaggt cgatttacgg gcttgattct gaaattgaat
     1321 tgatattatt tatttatcca tcatcgatgt ccctatttaa aaagaaagtc acttcgaaaa
     1381 ttgagcccgt aaatcgacac aagcattaca gtagtcattt aaaggattac tgtagtcttc
     1441 gctacgagat attttgcgcg tcaaatatgt tgtttaatac gcattttcag aattttaggt
     1501 tcccgtaata aatatttatc gatttccgct tttctcaaga atttattttt tattggaaaa
     1561 aaaagcatta aaaattcaat ttttaaattt tcagaaaaaa tagtgttttt ttaaatttaa
     1621 aattacatca agtacaaata tttgattttt gcgaataact ttacataaaa tttgaatcga
     1681 ttagtacctc tcttagcccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     1741 taacataaca tagaacattt tcaggaggac ccttgcttgg agggtaccga gctcagaaaa
     1801 aatgaccgct ccaaagaaga agcgcaaggt tccagtcgag aagatgactg ccccaaaaaa
     1861 gaaacgtaag gtcccgcggg taagtttaaa cagttcggta ctaactaacc atacatattt
     1921 aaattttcag cgcgctgtgt caaaaggaga agagcttatt aaggagaata tgcgttccaa
     1981 gctctacctc gagggatccg tcaacggaca ccaattcaag tgcacccacg agggagaggg
     2041 aaagccatac gagggaaagc aaaccaaccg tatcaaggtc gtcgagggag gaccactccc
     2101 attcgccttc gacatcctcg ccacccactt catgtacgga tccaaggtaa gtttaaacat
     2161 atatatacta actaaccctg attatttaaa ttttcaggtc ttcatcaagt acccagccga
     2221 cctcccagac tacttcaagc aatccttccc agagggattc acctgggagc gtgtcatggt
     2281 cttcgaggac ggaggagtcc tcaccgccac ccaagacacc tccctccaag acggagagct
     2341 catctacaac gtcaaggtcc gtggagtcaa cttcccagcc aacggaccag tcatgcaaaa
     2401 gaagaccctc ggatgggagc catccaccga gaccatgtac ccagccgacg gaggactcga
     2461 gggacgttgc gacaaggtaa gtttaaacat gattttacta actaactaat ctgatttaaa
     2521 ttttcaggcc ctcaagctcg tcggaggagg acacctccac gtcaacttca agaccaccta
     2581 caagtccaag aagccagtca agatgccagg agtccactac gtcgaccgtc gtctcgagcg
     2641 tatcaaggag gccgacaacg agacctacgt cgagcaatac gagcacgccg tcgcccgtta
     2701 ctccaacctc ggaggaggaa tggacgagct ctacaagcct aggataccgg tgggtgaaga
     2761 cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta cgaagccaaa
     2821 ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt
     2881 cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta tcatgaactc
     2941 ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg ctcactacaa
     3001 caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga tccttccagg
     3061 agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt acacttccag
     3121 caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa
     3181 ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa
     3241 tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     3301 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     3361 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     3421 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     3481 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     3541 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     3601 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     3661 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     3721 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     3781 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     3841 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     3901 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     3961 caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt cgtctcgcgc
     4021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4141 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4201 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     4261 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4321 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4381 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4441 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4621 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4681 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4861 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5281 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5341 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5401 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5581 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5641 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5701 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5761 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5821 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5881 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5941 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6001 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6061 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6121 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6181 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6241 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6301 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6361 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6421 atgttgtgtg gaattgtgag cggataacaa tt
//
