LOCUS       sequence-441795-        5710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srv-32 fluorescent neural reporter driving nuclear CyOFP1 expression
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY129
FEATURES             Location/Qualifiers
     source          1..5710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1069..1089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1111..1131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3301..3323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3423..3442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3520..3538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3606..3710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3711..3779,3780..4571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3929..3948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4742..5330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5231..5250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5484..5501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5618..5639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5654..5659,5660..5677,5678..5684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5692..5708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5697..5710,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aaatgtgaca
      181 acgtcgacaa aacgtgcaag taagaaagga attgatctat tgagaagatc ttcggaagaa
      241 ccaactccac aagttctaat tctataaaaa taaatttttt taaaattata aacgctttat
      301 caataaggtt tttgtagaat agcacaaaaa aataaaacga tgaaaaagtt ttgttttgaa
      361 aaatattaag caatccaata atgtttccta tcttttattt caaagtcctg tgctaaaaat
      421 agttattttc aaatcgtaag ctcaagtatg aaattaaaat ccatatttta attaattgag
      481 aaaaaatatt taacagattt aattgaaaat tgatagaaaa taaatgttgg tctctggact
      541 accacacaaa cacccctaaa agtgatttta taattaatct atgttatttt tttttactga
      601 tcaaacttta caacttgtta attttgggtt ggaagaatct tgattccaat ctaggtttca
      661 ttgaaccttt taataacatt tctgcctatg ttaaaatggt tcttgctctc cacttaagtt
      721 ctcacaccca aaattttctc acgtagttat ccttaaaaga ctcacttgtg gttcgaaaaa
      781 tgcttttcga cactttttgc ttcccagaaa tgactaacaa tatcttagtc agactcctag
      841 ttttttcctg tttcatattt tgtactaaac ttcgaaaaat tttttgcacg tatttattca
      901 tattctagat gattcggtct cagtttaaaa tcaacttttc taaaatattt ctagcccggg
      961 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     1021 aggaggaccc ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag
     1081 cgcaaggttc cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt cccgcgggta
     1141 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagcg cgctgtgtca
     1201 aaaggagaag agcttattaa ggagaatatg cgttccaagc tctacctcga gggatccgtc
     1261 aacggacacc aattcaagtg cacccacgag ggagagggaa agccatacga gggaaagcaa
     1321 accaaccgta tcaaggtcgt cgagggagga ccactcccat tcgccttcga catcctcgcc
     1381 acccacttca tgtacggatc caaggtaagt ttaaacatat atatactaac taaccctgat
     1441 tatttaaatt ttcaggtctt catcaagtac ccagccgacc tcccagacta cttcaagcaa
     1501 tccttcccag agggattcac ctgggagcgt gtcatggtct tcgaggacgg aggagtcctc
     1561 accgccaccc aagacacctc cctccaagac ggagagctca tctacaacgt caaggtccgt
     1621 ggagtcaact tcccagccaa cggaccagtc atgcaaaaga agaccctcgg atgggagcca
     1681 tccaccgaga ccatgtaccc agccgacgga ggactcgagg gacgttgcga caaggtaagt
     1741 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggccct caagctcgtc
     1801 ggaggaggac acctccacgt caacttcaag accacctaca agtccaagaa gccagtcaag
     1861 atgccaggag tccactacgt cgaccgtcgt ctcgagcgta tcaaggaggc cgacaacgag
     1921 acctacgtcg agcaatacga gcacgccgtc gcccgttact ccaacctcgg aggaggaatg
     1981 gacgagctct acaagcctag gataccggtg ggtgaagacc caccaaagcc atctgccaag
     2041 ggagccaaga aggccgccaa gaccgttacg aagccaaagg acggaaagaa gagacgtcat
     2101 gcccgtaagg aatcatactc cgtctacatc taccgtgtcc tcaagcaagt tcatccagac
     2161 actggagttt cctccaaagc catgtctatc atgaactctt ttgtcaacga tgtcttcgag
     2221 cgtattgctg ctgaagcatc ccgtcttgct cactacaaca agcgttccac aatctcatcc
     2281 cgcgaaattc agaccgctgt ccgtctgatc cttccaggag agcttgccaa gcacgccgtg
     2341 tctgagggaa ccaaggccgt taccaagtac acttccagca agtaggaatt ccaactgagc
     2401 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     2461 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     2521 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     2581 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     2641 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     2701 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     2761 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     2821 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     2881 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     2941 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     3001 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     3061 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     3121 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     3181 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     3241 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3421 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3481 atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt
     3541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4861 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5581 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5641 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5701 gataacaatt
//
