LOCUS       sequence-441796-        5454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srg-32 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY124
FEATURES             Location/Qualifiers
     source          1..5454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             819..839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             861..881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3045..3067)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3167..3186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3264..3282)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3350..3454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3455..3523,3524..4315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3673..3692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4486..5074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4975..4994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5228..5245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5362..5383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5398..5403,5404..5421,5422..5428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5436..5452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5441..5454,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gatattccac
      181 actacgcaat caactctggt caaaacttcg gagtagacaa ctacgacatt ggaactttaa
      241 acctggcgga tcaattcagt ggagaaacta ctgattgtat tcattagcaa tttatcgcac
      301 tgatctctga agtttaataa atatgcactt catttgctaa agtttaaatg atgaactaga
      361 catagacatt taagggaatt gcgtcaacat gaatattata ttttagaatt gaaaataagt
      421 ttaactttgc aatattatga aaacaaaaaa aggaaaaaag tttccaggaa ctgttttgat
      481 tccagattat gataatattt gtattatccg gaagttatgt tctccagtca aaaacataac
      541 tgttcacatg acacttcctc catctaacac cttcagaata aaatacgtca gagtatatgc
      601 tgaatagaac tcttctgtgc taattggctc atcgaccttt gggattgtct ggtcagaacg
      661 ctgacttcaa gttctccttt ctggttcatt tattcagttt gaaacccggg attggccaaa
      721 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
      781 ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag cgcaaggttc
      841 cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt cccgcggatg tcagagctta
      901 ttaaggagaa tatgcatatg aaactgtaca tggaaggaac cgtggacaac catcacttta
      961 agtgcacttc ggagggggaa ggcaaaccgt acgagggcac ccagaccatg cgaatcaagg
     1021 tggtcgaggg aggcccactg cccttcgcct tcgacattct ggtaagttta aacatatata
     1081 tactaactaa ccctgattat ttaaattttc aggctactag cttcctgtac ggcagcaaga
     1141 cttttattaa tcacacccag ggcatcccgg acttcttcaa acagtcgttc cctgagggct
     1201 tcacatggga gcgtgtgacc acatacgaag acggtggcgt tctcaccgct acccaggaca
     1261 ccagcctcca ggacggttgc ctcatatata atgtggtaag tttaaacagt tcggtactaa
     1321 ctaaccatac atatttaaat tttcagaaga tccggggagt aaacttcaca tccaatggtc
     1381 cggttatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg tatccggctg
     1441 acggaggcct ggaaggtcga aacgacatgg ccctgaagct cgtcggcggt agccatctca
     1501 tcgcaaacgc taagaccaca tatagatcca agaaaccggt aagtttaaac atgattttac
     1561 taactaacta atctgattta aattttcagg ctaagaacct taaaatgccg ggagtctact
     1621 atgttgacta cagactggaa cggatcaagg aggccaacaa cgagacctac gtcgagcagc
     1681 acgaggttgc agttgccaga tactgcgacc tcccgagcaa actgggtcac aagcttaatc
     1741 ctaggatacc ggtgggtgaa gacccaccaa agccatctgc caagggagcc aagaaggccg
     1801 ccaagaccgt tacgaagcca aaggacggaa agaagagacg tcatgcccgt aaggaatcat
     1861 actccgtcta catctaccgt gtcctcaagc aagttcatcc agacactgga gtttcctcca
     1921 aagccatgtc tatcatgaac tcttttgtca acgatgtctt cgagcgtatt gctgctgaag
     1981 catcccgtct tgctcactac aacaagcgtt ccacaatctc atcccgcgaa attcagaccg
     2041 ctgtccgtct gatccttcca ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg
     2101 ccgttaccaa gtacacttcc agcaagtagg aattccaact gagcgccggt cgctaccatt
     2161 accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg
     2221 agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac
     2281 ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     2341 ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag
     2401 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     2461 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     2521 ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa
     2581 aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat
     2641 ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg
     2701 agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc
     2761 tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa
     2821 tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc
     2881 agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc
     2941 cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg
     3001 tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3061 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3121 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3181 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     3241 taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3301 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3961 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4081 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4141 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4201 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4261 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5221 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     5281 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     5341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     5401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
